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Q1CK03

- NAPA_YERPN

UniProt

Q1CK03 - NAPA_YERPN

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Yersinia pestis bv. Antiqua (strain Nepal516)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (11 Jul 2006)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi81 – 811Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciYPES377628:GIXK-1399-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:YPN_1347
    ORF Names:YP516_1484
    OrganismiYersinia pestis bv. Antiqua (strain Nepal516)
    Taxonomic identifieri377628 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000008936: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
    BLAST
    Chaini32 – 830799Periplasmic nitrate reductasePRO_0000256078Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi377628.YPN_1347.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1CK03.
    SMRiQ1CK03. Positions 38-827.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiHPKTRVA.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    PF10518. TAT_signal. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q1CK03-1 [UniParc]FASTAAdd to Basket

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    MKLSRRDFMK ANAAVAAAAA AGMTIPTVAK AVGETTNAIK WDKAPCRFCG    50
    TGCGVLVGTQ NGRIVASQGD PDSPVNRGLN CIKGYFLPKI MYGKDRLTQP 100
    LLRMKDGQYD KEGDFTPISW EKAFDIMELK FKNALKEKGP TAVGMFGSGQ 150
    WTVWEGYAAL KLLKGGFRSN NLDPNARHCM ASSVVGFMRT FGMDEPMGCY 200
    DDIEEADAFV LWGSNMAEMH PVLWSRMTSR RLTNAHVRIA VLSTYEHRSF 250
    ELADNPIVFT PQTDLVIMNY IANYIIQNNA VDKDFLAQHV NFRRGATDIG 300
    YGLRPTHPLE KAAKNPGSDA SEPMSFEDFK TFVAEYTLEK AAKMSGVPED 350
    QLESLAQLYA DPKVKLVSYW TMGFNQHTRG VWANNMCYNL HLLTGKISTP 400
    GSGPFSLTGQ PSACGTAREV GTFSHRLPAD MVVTNEKHRQ IAETTWQLPA 450
    GTIPEKVGLH AVAQDRALKD GTLNAYWVMC NNNMQAGPNI NEERMPGWRD 500
    PRNFIVVSDP YPTISALSAD LILPTSMWVE KEGAYGNAER RTQFWRQQVP 550
    SPGEAKSDLW QIVEFAKRFN VEEVWPAELV NQKPEYRGKN LYEVLFANDV 600
    VSKYPLSEIP DDQLNDEARD FGFYIQKGLF EEYASFGRGH AHDLAPFDVY 650
    HQVRGLRWPV VDGKETLWRY REGFDPFVPK GEEVRFYGKP DGKAVIFALP 700
    YEPAAESPDQ EYDLWLSTGR VLEHWHTGSM TRRVPELHRA FPEAVLFIHP 750
    LDAKARGLHR GDKVKVISRR GEVISLVETR GRNRPPRGLV YMPFFDAAQL 800
    VNNLTLDATD PLSKETDFKK CAVKLERVVA 830
    Length:830
    Mass (Da):93,226
    Last modified:July 11, 2006 - v1
    Checksum:iC83E1665F370EF6D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000305 Genomic DNA. Translation: ABG17677.1.
    ACNQ01000008 Genomic DNA. Translation: EEO77797.1.
    RefSeqiYP_647277.1. NC_008149.1.

    Genome annotation databases

    EnsemblBacteriaiABG17677; ABG17677; YPN_1347.
    EEO77797; EEO77797; YP516_1484.
    GeneIDi4125970.
    KEGGiypn:YPN_1347.
    PATRICi18602009. VBIYerPes46733_1671.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000305 Genomic DNA. Translation: ABG17677.1 .
    ACNQ01000008 Genomic DNA. Translation: EEO77797.1 .
    RefSeqi YP_647277.1. NC_008149.1.

    3D structure databases

    ProteinModelPortali Q1CK03.
    SMRi Q1CK03. Positions 38-827.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 377628.YPN_1347.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABG17677 ; ABG17677 ; YPN_1347 .
    EEO77797 ; EEO77797 ; YP516_1484 .
    GeneIDi 4125970.
    KEGGi ypn:YPN_1347.
    PATRICi 18602009. VBIYerPes46733_1671.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi HPKTRVA.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci YPES377628:GIXK-1399-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    PF10518. TAT_signal. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen."
      Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L.
      J. Bacteriol. 188:4453-4463(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Nepal516.
    2. "Yersinia pestis Nepal516A whole genome shotgun sequencing project."
      Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L., Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R., Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S.
      Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiNAPA_YERPN
    AccessioniPrimary (citable) accession number: Q1CK03
    Secondary accession number(s): C4GRV6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: July 11, 2006
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3