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Q1CI76

- BGAL_YERPN

UniProt

Q1CI76 - BGAL_YERPN

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Yersinia pestis bv. Antiqua (strain Nepal516)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer.UniRule annotation
Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101SubstrateUniRule annotation
Metal bindingi209 – 2091SodiumUniRule annotation
Binding sitei209 – 2091SubstrateUniRule annotation
Sitei373 – 3731Transition state stabilizerUniRule annotation
Sitei407 – 4071Transition state stabilizerUniRule annotation
Metal bindingi432 – 4321Magnesium 1UniRule annotation
Metal bindingi434 – 4341Magnesium 1UniRule annotation
Active sitei477 – 4771Proton donorUniRule annotation
Metal bindingi477 – 4771Magnesium 1UniRule annotation
Binding sitei477 – 4771SubstrateUniRule annotation
Active sitei553 – 5531NucleophileUniRule annotation
Metal bindingi613 – 6131Magnesium 2UniRule annotation
Metal bindingi617 – 6171Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi620 – 6201SodiumUniRule annotation
Binding sitei620 – 6201SubstrateUniRule annotation
Binding sitei1035 – 10351SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciYPES377628:GIXK-2036-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:YPN_1975
ORF Names:YP516_2199
OrganismiYersinia pestis bv. Antiqua (strain Nepal516)
Taxonomic identifieri377628 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000008936: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10601060Beta-galactosidasePRO_0000367019Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi377628.YPN_1975.

Structurei

3D structure databases

ProteinModelPortaliQ1CI76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni553 – 5564Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiNPPFVPK.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1CI76-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSQEKVPLQ VQLSLPQILS RRDWENPQIT QYHRLEAHPP FHSWRDVESA
60 70 80 90 100
QKDRPSPQQQ TLNGLWSFSY FTQPEAVPEH WVRCDLAEAK PLPVPANWQL
110 120 130 140 150
HGYDAPIYTN IQYPIPVNPP RVPDLNPTGC YSRDFTLEPS WLASGKTRII
160 170 180 190 200
FDGVSSAFYL WCNGQWVGYS QDSRLPAEFD LTPYLQAGSN RIAVLVLRWS
210 220 230 240 250
DGSYLEDQDM WRMSGIFRDV KLLHKPEIHL RDIHIMTHLS PEFTSANLEV
260 270 280 290 300
MAAVNIPSLQ LNDPQVTGSY QLRVQLWLAD KLVASLQQPL GTQAIDERGP
310 320 330 340 350
YTDRTQLVLR IDQPLLWSAE QPTLYRAVVS LLNHQQELIE AEAYDVGFRQ
360 370 380 390 400
VAIHQGLLKI NGKAVLIRGV NRHEHHPQTG QAIDEESLLQ DILLMKQHNF
410 420 430 440 450
NAVRCSHYPN HPLWYRLCDR YGLYVVDEAN IETHGMQPMS RLSDDPSWFS
460 470 480 490 500
AFSERVTRMV QRDRNHPCII IWSLGNESGH GATHDALYRW IKTNDPTRPV
510 520 530 540 550
QYEGGGANTL ATDILCPMYA RVDEDQPFPA VPKWSIKKWI GLPNESRPLI
560 570 580 590 600
LCEYAHAMGN SFGGFARYWQ AFRQYPRLQG GFIWDWVDQS LTHHNDHGQP
610 620 630 640 650
YWAYGGDFGD TPNDRQFCMN GLVFPDRSPH PSLYEAQCAQ QFFQFSLLST
660 670 680 690 700
TPLVINITSE YLFRESDNEQ LYWRIMLEGE SVLEGSQPLN LSPESSQCYR
710 720 730 740 750
LAEKLPTLNK PGQLWLNVEI RQPKETPWSP AQHRSAWHQW RLPQPLFSPS
760 770 780 790 800
SDLTNATAHY APQLQHNLQL QHDLQLQQDE QHIKVTYQQQ CWQFSRQTGR
810 820 830 840 850
LAQWWVADKP MLLRPLQDQF VRAPLDNDIG ISEATHIDPN AWVERWKKAG
860 870 880 890 900
MYQLQQRCLS LHVDHLSHSV QISAEYGYEF EQEPLLHSHW VYRFDRHGRM
910 920 930 940 950
TIDVNVRIAT SLPAPARIGM CCQLADISPT VEWLGLGPHE NYPDRQLAAQ
960 970 980 990 1000
YGHWSLPLEQ MHTAYIFPSE NGLRCNTHTL NYGRWTLTGD FHFGISRYST
1010 1020 1030 1040 1050
QQLMVTSHQH LLEPEEGTWL NIDGFHMGVG GDDSWSPSVH IDDILTRETY
1060
QYQICWQYKV
Length:1,060
Mass (Da):122,625
Last modified:July 11, 2006 - v1
Checksum:i1142D7FC68E46697
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000305 Genomic DNA. Translation: ABG18304.1.
ACNQ01000011 Genomic DNA. Translation: EEO76599.1.
RefSeqiYP_647904.1. NC_008149.1.

Genome annotation databases

EnsemblBacteriaiABG18304; ABG18304; YPN_1975.
EEO76599; EEO76599; YP516_2199.
GeneIDi4122748.
KEGGiypn:YPN_1975.
PATRICi18603438. VBIYerPes46733_2379.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000305 Genomic DNA. Translation: ABG18304.1 .
ACNQ01000011 Genomic DNA. Translation: EEO76599.1 .
RefSeqi YP_647904.1. NC_008149.1.

3D structure databases

ProteinModelPortali Q1CI76.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 377628.YPN_1975.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG18304 ; ABG18304 ; YPN_1975 .
EEO76599 ; EEO76599 ; YP516_2199 .
GeneIDi 4122748.
KEGGi ypn:YPN_1975.
PATRICi 18603438. VBIYerPes46733_2379.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi NPPFVPK.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci YPES377628:GIXK-2036-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen."
    Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L.
    J. Bacteriol. 188:4453-4463(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nepal516.
  2. "Yersinia pestis Nepal516A whole genome shotgun sequencing project."
    Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L., Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R., Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S.
    Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiBGAL_YERPN
AccessioniPrimary (citable) accession number: Q1CI76
Secondary accession number(s): C4GTT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 11, 2006
Last modified: October 1, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3