Q1CI48 (NAGK_YERPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyl-D-glucosamine kinase
EC=2.7.1.59

Alternative name(s):
GlcNAc kinase

Gene names

Name:	nagK
Ordered Locus Names:	YPN_2003
ORF Names:	YP516_2229

Organism

Yersinia pestis bv. Antiqua (strain Nepal516) [Complete proteome] [HAMAP]

Taxonomic identifier

377628 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pestis

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P By similarity. HAMAP-Rule MF_01271
Catalytic activity	ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate. HAMAP-Rule MF_01271
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_01271
Miscellaneous	Might be inactive, since the sequence is shorter than in other family members, due to a probable natural frameshift in the C-terminus. HAMAP-Rule MF_01271
Sequence similarities	Belongs to the ROK (NagC/XylR) family. NagK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidoglycan turnover Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW N-acetylglucosamine kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	N-acetyl-D-glucosamine kinase HAMAP-Rule MF_01271		PRO_0000270126
Regions
	Nucleotide binding	4 – 11	8	ATP Potential
	Nucleotide binding	133 – 140	8	ATP Potential
Sites
	Metal binding	157	1	Zinc By similarity
	Metal binding	177	1	Zinc By similarity
	Metal binding	179	1	Zinc By similarity
	Metal binding	184	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1CI48 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 0BCE117500ABD548	FASTA	256	27,879
	10 20 30 40 50 60 MYYGFDMGGT KIELGVFDEN LQRIWHKRVP TPREDYPQLL QILRDLTEEA DTYCGVQGSV 70 80 90 100 110 120 GIGIPGLPNA DDGTVFTANV PSAMGQPLQA DLSRLIQREV RIDNDANCFA LSEAWDPEFR 130 140 150 160 170 180 TYPTVLGLIL GTGVGGGLIV NGSIVSGRNH ITGEFGHFRL PVDALDILGA DIPRVPCGCG 190 200 210 220 230 240 HRGCIENYIS GRGFEWMYSH FYQHTLPATD IIAHYAAGEP KAVAHVERFM DVLAVCLGNL 250 LTMLGSPFGR GGWGVV

References

[1]	"Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen." Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L. J. Bacteriol. 188:4453-4463(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nepal516.
[2]	"Yersinia pestis Nepal516A whole genome shotgun sequencing project." Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L., Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R., Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S. Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000305 Genomic DNA. Translation: ABG18332.1. ACNQ01000011 Genomic DNA. Translation: EEO76629.1.
RefSeq	YP_647932.1. NC_008149.1.
3D structure databases
ProteinModelPortal	Q1CI48.
SMR	Q1CI48. Positions 1-244.
ModBase	Search...
Protein-protein interaction databases
STRING	377628.YPN_2003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABG18332; ABG18332; YPN_2003. EEO76629; EEO76629; YP516_2229.
GeneID	4123080.
KEGG	ypn:YPN_2003.
PATRIC	18603496. VBIYerPes46733_2408.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1940.
HOGENOM	HOG000150087.
KO	K00884.
OMA	FGHIRLP.
ProtClustDB	PRK13311.
Enzyme and pathway databases
UniPathway	UPA00544.
Family and domain databases
HAMAP	MF_01271. GlcNAc_kinase. Divergent sequence.
InterPro	IPR000600. ROK. [Graphical view]
Pfam	PF00480. ROK. 1 hit. [Graphical view]
PROSITE	PS01125. ROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGK_YERPN

Accession

Primary (citable) accession number: Q1CI48
Secondary accession number(s): C4GTW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	July 11, 2006
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program