Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dihydroorotase

Gene

pyrC

Organism
Yersinia pestis bv. Antiqua (strain Nepal516)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathway:iUMP biosynthesis via de novo pathway

This protein is involved in step 3 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi17 – 171Zinc 1; via tele nitrogenUniRule annotation
Metal bindingi19 – 191Zinc 1; via tele nitrogenUniRule annotation
Metal bindingi103 – 1031Zinc 1; via carbamate groupUniRule annotation
Metal bindingi103 – 1031Zinc 2; via carbamate groupUniRule annotation
Metal bindingi140 – 1401Zinc 2; via pros nitrogenUniRule annotation
Metal bindingi178 – 1781Zinc 2; via tele nitrogenUniRule annotation
Metal bindingi251 – 2511Zinc 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYPES377628:GIXK-2103-MONOMER.
UniPathwayiUPA00070; UER00117.

Names & Taxonomyi

Protein namesi
Recommended name:
DihydroorotaseUniRule annotation (EC:3.5.2.3UniRule annotation)
Short name:
DHOaseUniRule annotation
Gene namesi
Name:pyrCUniRule annotation
Ordered Locus Names:YPN_2042
ORF Names:YP516_2274
OrganismiYersinia pestis bv. Antiqua (strain Nepal516)
Taxonomic identifieri377628 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000008936 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348DihydroorotasePRO_1000024075Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei103 – 1031N6-carboxylysineUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ1CI09.
SMRiQ1CI09. Positions 6-347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DHOase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0418.
HOGENOMiHOG000256259.
OMAiYAEAFEQ.
OrthoDBiEOG6TFCMH.

Family and domain databases

HAMAPiMF_00219. PyrC_type1.
InterProiIPR006680. Amidohydro_1.
IPR004721. DHOdimr.
IPR002195. Dihydroorotase_CS.
[Graphical view]
PANTHERiPTHR11647:SF59. PTHR11647:SF59. 1 hit.
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001237. DHOdimr. 1 hit.
TIGRFAMsiTIGR00856. pyrC_dimer. 1 hit.
PROSITEiPS00483. DIHYDROOROTASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1CI09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAQPQTLKI RRPDDWHIHL RDDEMLSTVL PYTSEVFARA IVMPNLAQPI
60 70 80 90 100
TTVASAIAYR ERILAAVPAG HKFTPLMTCY LTNSLDAKEL TTGFEQGVFT
110 120 130 140 150
AAKLYPANAT TNSTHGVSDI PAIYPLFEQM QKIGMPLLIH GEVTDAAVDI
160 170 180 190 200
FDREARFIDQ ILEPIRQKFP ELKIVFEHIT TKDAADYVLA GNRFLGATVT
210 220 230 240 250
PQHLMFNRNH MLVGGIRPHL FCLPILKRST HQQALRAAVA SGSDRFFLGT
260 270 280 290 300
DSAPHAKHRK ESSCGCAGVF NAPAALPAYA SVFEELNALQ HLEAFCALNG
310 320 330 340
PRFYGLPVND DVVELVRTPF LQPEEIPLGN ESVIPFLAGQ TLNWSVKR
Length:348
Mass (Da):38,542
Last modified:July 11, 2006 - v1
Checksum:iB9215D6E02697221
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000305 Genomic DNA. Translation: ABG18371.1.
ACNQ01000012 Genomic DNA. Translation: EEO76520.1.
RefSeqiWP_002213109.1. NZ_ACNQ01000012.1.

Genome annotation databases

EnsemblBacteriaiABG18371; ABG18371; YPN_2042.
EEO76520; EEO76520; YP516_2274.
PATRICi18603582. VBIYerPes46733_2451.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000305 Genomic DNA. Translation: ABG18371.1.
ACNQ01000012 Genomic DNA. Translation: EEO76520.1.
RefSeqiWP_002213109.1. NZ_ACNQ01000012.1.

3D structure databases

ProteinModelPortaliQ1CI09.
SMRiQ1CI09. Positions 6-347.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG18371; ABG18371; YPN_2042.
EEO76520; EEO76520; YP516_2274.
PATRICi18603582. VBIYerPes46733_2451.

Phylogenomic databases

eggNOGiCOG0418.
HOGENOMiHOG000256259.
OMAiYAEAFEQ.
OrthoDBiEOG6TFCMH.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00117.
BioCyciYPES377628:GIXK-2103-MONOMER.

Family and domain databases

HAMAPiMF_00219. PyrC_type1.
InterProiIPR006680. Amidohydro_1.
IPR004721. DHOdimr.
IPR002195. Dihydroorotase_CS.
[Graphical view]
PANTHERiPTHR11647:SF59. PTHR11647:SF59. 1 hit.
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001237. DHOdimr. 1 hit.
TIGRFAMsiTIGR00856. pyrC_dimer. 1 hit.
PROSITEiPS00483. DIHYDROOROTASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen."
    Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L.
    J. Bacteriol. 188:4453-4463(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nepal516.
  2. "Yersinia pestis Nepal516A whole genome shotgun sequencing project."
    Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L., Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R., Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S.
    Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nepal516.

Entry informationi

Entry nameiPYRC_YERPN
AccessioniPrimary (citable) accession number: Q1CI09
Secondary accession number(s): C4GU08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 11, 2006
Last modified: July 22, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.