Reviewed, UniProtKB/Swiss-Prot Q1CGT4 (MAO1_YERPN)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	YPN_2468
ORF Names:	YP516_2781

Organism

Yersinia pestis bv. Antiqua (strain Nepal516) [Complete proteome] [HAMAP]

Taxonomic identifier

377628 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity. HAMAP MF_01619
Subunit structure	Homotetramer By similarity. HAMAP MF_01619
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	NAD-dependent malic enzyme HAMAP MF_01619		PRO_1000069555
Sites
	Active site	104	1	Proton donor By similarity
	Active site	175	1	Proton acceptor By similarity
	Metal binding	246	1	Divalent metal cation By similarity
	Metal binding	247	1	Divalent metal cation By similarity
	Metal binding	270	1	Divalent metal cation By similarity
	Binding site	157	1	NAD By similarity
	Binding site	270	1	NAD By similarity
	Binding site	418	1	NAD By similarity
	Site	270	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1CGT4-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: F57C1183EEC4AA3D	FASTA	565	62,828
	10 20 30 40 50 60 MELEYESKRP LYIPYAGPIL LEFPLLNKGS AFTNDERNHF NLHGLLPEAV ETIEEQAERA 70 80 90 100 110 120 YRQYQDFKND DDKHIYLRNI QDTNETLFYR LLEAHLSEMM PIIYTPTVGE ACEHFSDIYR 130 140 150 160 170 180 RARGLFISYP NREHIDDMLQ NATKQNVKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 190 200 210 220 230 240 ACGGISPAYT LPVVLDVGTN NPQRLNDPLY MGWRHPRISG DEYYAFVDEF IQAVKRRWPN 250 260 270 280 290 300 VLLQFEDFAQ KNATPLLNRY RDELCCFNDD IQGTAAVTLG SLIAASHAAG SQLRDQTVTF 310 320 330 340 350 360 LGAGSAGCGI AEQIIAQMMS EGLSEIQARA RIFMVDRFGL LTDKLPNLLD FQSKLVQKSD 370 380 390 400 410 420 DLHHWNLHND AISLLDVVRN AKPTVLIGVS GQPGLFTEEL IREMHSHCAR PIVMPLSNPT 430 440 450 460 470 480 SRVEARPEDI INWTDGAALV ATGSPFPPVS YKEKLYPIAQ CNNSYIFPGI GLGVLASGAS 490 500 510 520 530 540 RVTDGMLMAA SRALAESSPL ARHGEGALLP NIDDIQAVSK AIAMRVGQAA QLQGVAIVTS 550 560 EEALSKAIEH NYWQPQYRSY KRTSF

References

[1]	"Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen." Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L. J. Bacteriol. 188:4453-4463(2006) [PubMed: 16740952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Yersinia pestis Nepal516A whole genome shotgun sequencing project." Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L., Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R., Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S. Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000305 Genomic DNA. Translation: ABG18796.1. ACNQ01000014 Genomic DNA. Translation: EEO76034.1.
RefSeq	YP_648396.1.
3D structure databases
SMR	Q1CGT4. Positions 16-565.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1CGT4.
Genome annotation databases
GeneID	4122790.
GenomeReviews	Gene locus YPN_2468 in contig CP000305_GR.
KEGG	ypn:YPN_2468.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0281.
HOGENOM	HBG289821.
OMA	IQDNNET.
Enzyme and pathway databases
BioCyc	YPES377628:YPN_2468-MONOMER.
Family and domain databases
HAMAP	MF_01619. NAD_malic_enz. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_YERPN

Accession

Primary (citable) accession number: Q1CGT4
Secondary accession number(s): C4GVF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 11, 2006
Last modified:	January 19, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)