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Protein

tRNA pseudouridine synthase B

Gene

truB

Organism
Yersinia pestis bv. Antiqua (strain Nepal516)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431SubstrateUniRule annotation
Active sitei48 – 481NucleophileUniRule annotation
Binding sitei76 – 761SubstrateUniRule annotation
Binding sitei179 – 1791SubstrateUniRule annotation
Binding sitei200 – 2001SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciYPES377628:GIXK-3327-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)
Alternative name(s):
tRNA pseudouridine(55) synthaseUniRule annotation
Short name:
Psi55 synthaseUniRule annotation
tRNA pseudouridylate synthaseUniRule annotation
tRNA-uridine isomeraseUniRule annotation
Gene namesi
Name:truBUniRule annotation
Ordered Locus Names:YPN_3238
ORF Names:YP516_3678
OrganismiYersinia pestis bv. Antiqua (strain Nepal516)
Taxonomic identifieri377628 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
Proteomesi
  • UP000008936 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324tRNA pseudouridine synthase BPRO_1000084718Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ1CEL5.
SMRiQ1CEL5. Positions 10-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000231223.
KOiK03177.
OMAiYELQFIR.
OrthoDBiEOG6358D3.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR015240. tRNA_sdUridine_synth_fam1_C.
IPR032819. TruB_C.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF09157. TruB-C_2. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1CEL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRPRRRGRD INGVLLLDKP LGLSSNDVLQ KVKRLFSANR AGHTGALDPL
60 70 80 90 100
ATGMLPICLG EATKFSQFLL DSDKRYRVVA RLGQRTDTSD AEGALISERE
110 120 130 140 150
VNLTQAQIDT ALESFRGESQ QIPSMYSALK HQGKPLYEYA RQGIEVEREA
160 170 180 190 200
RSITVYELLF IRWEGNDLEL EIHCSKGTYI RTIIDDLGEL LGCGAHVSYL
210 220 230 240 250
RRLQVATYPS ERMVTLEQLT AMVEAAQAEG RSPNPELDSL LLPMDSAVLN
260 270 280 290 300
FPEVNLLPSV AAYVKQGQPV HVSGAPSEGM VRITEGKERN FIGIGTIAED
310 320
GRVAPKRLVV ESVEVENLPV ENKK
Length:324
Mass (Da):35,812
Last modified:July 11, 2006 - v1
Checksum:i4A4DB7A45A9FE724
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000305 Genomic DNA. Translation: ABG19565.1.
ACNQ01000017 Genomic DNA. Translation: EEO75745.1.
RefSeqiWP_002209256.1. NZ_ACNQ01000017.1.

Genome annotation databases

EnsemblBacteriaiABG19565; ABG19565; YPN_3238.
EEO75745; EEO75745; YP516_3678.
KEGGiypn:YPN_3238.
PATRICi18606417. VBIYerPes46733_3846.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000305 Genomic DNA. Translation: ABG19565.1.
ACNQ01000017 Genomic DNA. Translation: EEO75745.1.
RefSeqiWP_002209256.1. NZ_ACNQ01000017.1.

3D structure databases

ProteinModelPortaliQ1CEL5.
SMRiQ1CEL5. Positions 10-311.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG19565; ABG19565; YPN_3238.
EEO75745; EEO75745; YP516_3678.
KEGGiypn:YPN_3238.
PATRICi18606417. VBIYerPes46733_3846.

Phylogenomic databases

HOGENOMiHOG000231223.
KOiK03177.
OMAiYELQFIR.
OrthoDBiEOG6358D3.

Enzyme and pathway databases

BioCyciYPES377628:GIXK-3327-MONOMER.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR015240. tRNA_sdUridine_synth_fam1_C.
IPR032819. TruB_C.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF09157. TruB-C_2. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen."
    Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L.
    J. Bacteriol. 188:4453-4463(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nepal516.
  2. "Yersinia pestis Nepal516A whole genome shotgun sequencing project."
    Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L., Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R., Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S.
    Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nepal516.

Entry informationi

Entry nameiTRUB_YERPN
AccessioniPrimary (citable) accession number: Q1CEL5
Secondary accession number(s): C4GXU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 11, 2006
Last modified: January 20, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.