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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Yersinia pestis bv. Antiqua (strain Nepal516)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei405 – 4051NucleophileUniRule annotation
Active sitei458 – 4581Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciYPES377628:GIXK-3689-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:YPN_3590
ORF Names:YP516_4079
OrganismiYersinia pestis bv. Antiqua (strain Nepal516)
Taxonomic identifieri377628 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000008936 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7277271,4-alpha-glucan branching enzyme GlgBPRO_0000260721Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi377628.YPN_3590.

Structurei

3D structure databases

ProteinModelPortaliQ1CDL3.
SMRiQ1CDL3. Positions 117-726.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiEGHLYKY.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1CDL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVLPDRQVI NQLISGHYGD PFSILGMHET SQGLQICALL PDAREVWLVE
60 70 80 90 100
TENGRRIAQL TLEDPRGFFI AQLTRRKKSF RYQFAVTWQE NPQIIEDPYR
110 120 130 140 150
FGPLLQDIDS WLLAEGTHLR PYERLGAHLM SLDGVSGVSF AVWAPNAQRV
160 170 180 190 200
SVVGDFNFWD GRRHPMRLRR ENGIWELFLP GIEAGQLYKF EIIDCHGQVR
210 220 230 240 250
LKADPYAFEA QMRPETASLI SPLPDVVKSS AARQKANDLC SPVSIYEVHL
260 270 280 290 300
GSWRRHTDNN FWLSYRELAD QLVEYVKYMG FTHVELLPIN EHPFDGSWGY
310 320 330 340 350
QPLGLYAPTR RYGTPEDFKA FVAKFHQAGI NVILDWVPGH FPSDEHGLST
360 370 380 390 400
FDGTALYEYA DPREGYHQDW NTLIYNYGRN EVRNYLAGNA FYWMERFGID
410 420 430 440 450
ALRIDAVASM IYRDYSRAEG QWVPNYYGGR ENLEAIAFLR YTNKTIGVER
460 470 480 490 500
PGSVTMAEES TDFPGVTLPP DIGGLGFNYK WNMGWMHDTL NYMQCDPVHR
510 520 530 540 550
KYHHNLMTFG MLYAYTENFI LPLSHDEVVH GKRSILDRMP GDAWQKFANL
560 570 580 590 600
RAYYAFMWAH PGKKLLFMGC EFAQGREWNF ETSLDWHLLD DENGWHSGVQ
610 620 630 640 650
RLVRDLNHCY RQYAPLYEWD YQPAGFEWLV VDDHENSVFA FLRRDAEGHE
660 670 680 690 700
LIAISNFTPV PRYHYRVGIP QGGHYREVLN SDSAFYCGSN LGNQGGIDSH
710 720
HVRSHNHEHS LLLTLPPLAT IYLLREN
Length:727
Mass (Da):84,135
Last modified:July 10, 2006 - v1
Checksum:i40D16ED7083B33D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000305 Genomic DNA. Translation: ABG19917.1.
ACNQ01000019 Genomic DNA. Translation: EEO74482.1.
RefSeqiYP_649517.1. NC_008149.1.

Genome annotation databases

EnsemblBacteriaiABG19917; ABG19917; YPN_3590.
EEO74482; EEO74482; YP516_4079.
GeneIDi4124595.
KEGGiypn:YPN_3590.
PATRICi18607235. VBIYerPes46733_4247.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000305 Genomic DNA. Translation: ABG19917.1.
ACNQ01000019 Genomic DNA. Translation: EEO74482.1.
RefSeqiYP_649517.1. NC_008149.1.

3D structure databases

ProteinModelPortaliQ1CDL3.
SMRiQ1CDL3. Positions 117-726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi377628.YPN_3590.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG19917; ABG19917; YPN_3590.
EEO74482; EEO74482; YP516_4079.
GeneIDi4124595.
KEGGiypn:YPN_3590.
PATRICi18607235. VBIYerPes46733_4247.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiEGHLYKY.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciYPES377628:GIXK-3689-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen."
    Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L.
    J. Bacteriol. 188:4453-4463(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Nepal516.
  2. "Yersinia pestis Nepal516A whole genome shotgun sequencing project."
    Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L., Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R., Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiGLGB_YERPN
AccessioniPrimary (citable) accession number: Q1CDL3
Secondary accession number(s): D1Q1S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 27, 2006
Last sequence update: July 10, 2006
Last modified: January 6, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.