Q1CCX5 (FMT_YERPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	YPN_3828
ORF Names:	YP516_4350

Organism

Yersinia pestis bv. Antiqua (strain Nepal516) [Complete proteome] [HAMAP]

Taxonomic identifier

377628 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pestis

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the Fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_1000020208
Regions
	Region	113 – 116	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1CCX5 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 24F167229DC2E0C8	FASTA	315	34,140
	10 20 30 40 50 60 MSDSLRIIFA GTPDFAARHL GALLSSQHKI VGVFTQPDRP AGRGNKLTPS PVKILAEHHG 70 80 90 100 110 120 IPVFQPKSLR PEENQHLVAD LNADIMVVVA YGLILPAAVL AMPRLGCINV HGSLLPRWRG 130 140 150 160 170 180 AAPIQRSVWA GDEKTGITIM QMDIGLDTGA MLHKIECAIQ PEDTSATLYD KLAQLGPQGL 190 200 210 220 230 240 LITLQQLAAG TALAEVQNET QATYAEKLSK EEAKLDWTLS ATQLERCIRA FNPWPVSYFI 250 260 270 280 290 300 VDEQPIKVWQ AQVLPAGEDA EPGTIIHADK HGIQVATADG VLNITQLQPA GKKAMSAADL 310 LNSRREWFIP GSQLV

References

[1]	"Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen." Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L. J. Bacteriol. 188:4453-4463(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nepal516.
[2]	"Yersinia pestis Nepal516A whole genome shotgun sequencing project." Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L., Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R., Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S. Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000305 Genomic DNA. Translation: ABG20155.1. ACNQ01000019 Genomic DNA. Translation: EEO74742.1.
RefSeq	YP_649755.1. NC_008149.1.
3D structure databases
ProteinModelPortal	Q1CCX5.
SMR	Q1CCX5. Positions 2-315.
ModBase	Search...
Protein-protein interaction databases
STRING	377628.YPN_3828.
Proteomic databases
PRIDE	Q1CCX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABG20155; ABG20155; YPN_3828. EEO74742; EEO74742; YP516_4350.
GeneID	4123299.
KEGG	ypn:YPN_3828.
PATRIC	18607783. VBIYerPes46733_4519.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	GITLMQM.
ProtClustDB	PRK00005.
Enzyme and pathway databases
BioCyc	YPES377628:GIXK-3929-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR001555. GART_AS. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_YERPN

Accession

Primary (citable) accession number: Q1CCX5
Secondary accession number(s): D1Q2I9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 11, 2006
Last modified:	May 29, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program