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Q1CCP6

- CYSG2_YERPN

UniProt

Q1CCP6 - CYSG2_YERPN

Protein

Siroheme synthase 2

Gene

cysG2

Organism
Yersinia pestis bv. Antiqua (strain Nepal516)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (11 Jul 2006)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
    S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
    Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
    Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei222 – 2221S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei245 – 2451Proton acceptorUniRule annotation
    Active sitei267 – 2671Proton donorUniRule annotation
    Binding sitei303 – 3031S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei379 – 3791S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
    Binding sitei408 – 4081S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi22 – 232NADUniRule annotation
    Nucleotide bindingi43 – 442NADUniRule annotation

    GO - Molecular functioni

    1. NAD binding Source: InterPro
    2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
    3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
    4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
    2. siroheme biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase, Methyltransferase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Cobalamin biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    NAD, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciYPES377628:GIXK-4008-MONOMER.
    UniPathwayiUPA00148; UER00211.
    UPA00148; UER00222.
    UPA00262; UER00211.
    UPA00262; UER00222.
    UPA00262; UER00376.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Siroheme synthase 2UniRule annotation
    Including the following 3 domains:
    Uroporphyrinogen-III C-methyltransferase 2UniRule annotation (EC:2.1.1.107UniRule annotation)
    Short name:
    Urogen III methylase 2UniRule annotation
    Alternative name(s):
    SUMT 2UniRule annotation
    Uroporphyrinogen III methylase 2UniRule annotation
    Short name:
    UROM 2UniRule annotation
    Precorrin-2 dehydrogenase 2UniRule annotation (EC:1.3.1.76UniRule annotation)
    Sirohydrochlorin ferrochelatase 2UniRule annotation (EC:4.99.1.4UniRule annotation)
    Gene namesi
    Name:cysG2UniRule annotation
    Ordered Locus Names:YPN_3907
    ORF Names:YP516_4437
    OrganismiYersinia pestis bv. Antiqua (strain Nepal516)
    Taxonomic identifieri377628 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000008936: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 470470Siroheme synthase 2PRO_0000330575Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei128 – 1281PhosphoserineUniRule annotation

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi377628.YPN_3907.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1CCP6.
    SMRiQ1CCP6. Positions 1-453.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 201201precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd
    BLAST
    Regioni213 – 470258Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd
    BLAST
    Regioni298 – 3003S-adenosyl-L-methionine bindingUniRule annotation
    Regioni328 – 3292S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation
    In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0007.
    HOGENOMiHOG000290518.
    KOiK02302.
    OMAiQASFIMP.
    OrthoDBiEOG6DRPFR.

    Family and domain databases

    Gene3Di1.10.8.210. 1 hit.
    3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPiMF_01646. Siroheme_synth.
    InterProiIPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR016040. NAD(P)-bd_dom.
    IPR012409. Sirohaem_synth.
    IPR019478. Sirohaem_synthase_dimer_dom.
    IPR006367. Sirohaem_synthase_N.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view]
    PfamiPF10414. CysG_dimeriser. 1 hit.
    PF13241. NAD_binding_7. 1 hit.
    PF00590. TP_methylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
    SUPFAMiSSF53790. SSF53790. 1 hit.
    TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
    TIGR01470. cysG_Nterm. 1 hit.
    PROSITEiPS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1CCP6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYFPIFCQL QHKACLLVGG GEIAERKARL LLDAGALVTV NACEFTPQFH    50
    HWADQGQLSL ISGEFVPELL ADKWLVIAAT DQLSVNALVY QSANQQRIFC 100
    NVVDDPKRTS FIMPSIIDRS PIMIAVSSGG KAPVLARLLR EKLEALLPQH 150
    LGQLAGNLRQ RVKQHFTVMT ERRRFWEKLL THDRLAQSLA NNDHVQADQH 200
    VEQLFSAPLT DRGEVVLVGA GPGDAGLLTL KGLQQIQQAD VVVYDRLVSD 250
    EVMNLVRRDA ERIFVGKQSG HHCVPQEQIN QILLQQAQSG KRVVRLKGGD 300
    PFIFGRGGEE LEELAGYGIP FSVVPGITAA SGCSAYSGIP LTHRDHAQSV 350
    RLVTGHAKKE GQLDWANLAA EKQTLVFYMG LSQAGEIQQQ LIQHGMPATT 400
    QVALVENGTS RHQRVVSGEL SQLALLSQQV SSPSLIIVGS VVSLREKLNW 450
    FSSRHHDDQP KVTECVAHVG 470
    Length:470
    Mass (Da):51,664
    Last modified:July 11, 2006 - v1
    Checksum:iD28E9E9C1182F316
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000305 Genomic DNA. Translation: ABG20234.1.
    ACNQ01000019 Genomic DNA. Translation: EEO74827.1.
    RefSeqiYP_649834.1. NC_008149.1.

    Genome annotation databases

    EnsemblBacteriaiABG20234; ABG20234; YPN_3907.
    EEO74827; EEO74827; YP516_4437.
    GeneIDi4125659.
    KEGGiypn:YPN_3907.
    PATRICi18607949. VBIYerPes46733_4602.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000305 Genomic DNA. Translation: ABG20234.1 .
    ACNQ01000019 Genomic DNA. Translation: EEO74827.1 .
    RefSeqi YP_649834.1. NC_008149.1.

    3D structure databases

    ProteinModelPortali Q1CCP6.
    SMRi Q1CCP6. Positions 1-453.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 377628.YPN_3907.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABG20234 ; ABG20234 ; YPN_3907 .
    EEO74827 ; EEO74827 ; YP516_4437 .
    GeneIDi 4125659.
    KEGGi ypn:YPN_3907.
    PATRICi 18607949. VBIYerPes46733_4602.

    Phylogenomic databases

    eggNOGi COG0007.
    HOGENOMi HOG000290518.
    KOi K02302.
    OMAi QASFIMP.
    OrthoDBi EOG6DRPFR.

    Enzyme and pathway databases

    UniPathwayi UPA00148 ; UER00211 .
    UPA00148 ; UER00222 .
    UPA00262 ; UER00211 .
    UPA00262 ; UER00222 .
    UPA00262 ; UER00376 .
    BioCyci YPES377628:GIXK-4008-MONOMER.

    Family and domain databases

    Gene3Di 1.10.8.210. 1 hit.
    3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPi MF_01646. Siroheme_synth.
    InterProi IPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR016040. NAD(P)-bd_dom.
    IPR012409. Sirohaem_synth.
    IPR019478. Sirohaem_synthase_dimer_dom.
    IPR006367. Sirohaem_synthase_N.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view ]
    Pfami PF10414. CysG_dimeriser. 1 hit.
    PF13241. NAD_binding_7. 1 hit.
    PF00590. TP_methylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
    SUPFAMi SSF53790. SSF53790. 1 hit.
    TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
    TIGR01470. cysG_Nterm. 1 hit.
    PROSITEi PS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen."
      Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L.
      J. Bacteriol. 188:4453-4463(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Nepal516.
    2. "Yersinia pestis Nepal516A whole genome shotgun sequencing project."
      Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L., Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R., Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S.
      Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiCYSG2_YERPN
    AccessioniPrimary (citable) accession number: Q1CCP6
    Secondary accession number(s): D1Q2S4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: July 11, 2006
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3