Q1C5S8 (NAPA_YERPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Periplasmic nitrate reductase
EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	YPA_2229

Organism

Yersinia pestis bv. Antiqua (strain Antiqua) [Complete proteome] [HAMAP]

Taxonomic identifier

360102 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pestis

Protein attributes

Sequence length	830 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with NapB By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Tat-type signal Potential
	Chain	32 – 830	799	Periplasmic nitrate reductase HAMAP-Rule MF_01630		PRO_0000256077
Sites
	Metal binding	46	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	49	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	53	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	81	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1C5S8 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: C83E1665F370EF6D	FASTA	830	93,226
	10 20 30 40 50 60 MKLSRRDFMK ANAAVAAAAA AGMTIPTVAK AVGETTNAIK WDKAPCRFCG TGCGVLVGTQ 70 80 90 100 110 120 NGRIVASQGD PDSPVNRGLN CIKGYFLPKI MYGKDRLTQP LLRMKDGQYD KEGDFTPISW 130 140 150 160 170 180 EKAFDIMELK FKNALKEKGP TAVGMFGSGQ WTVWEGYAAL KLLKGGFRSN NLDPNARHCM 190 200 210 220 230 240 ASSVVGFMRT FGMDEPMGCY DDIEEADAFV LWGSNMAEMH PVLWSRMTSR RLTNAHVRIA 250 260 270 280 290 300 VLSTYEHRSF ELADNPIVFT PQTDLVIMNY IANYIIQNNA VDKDFLAQHV NFRRGATDIG 310 320 330 340 350 360 YGLRPTHPLE KAAKNPGSDA SEPMSFEDFK TFVAEYTLEK AAKMSGVPED QLESLAQLYA 370 380 390 400 410 420 DPKVKLVSYW TMGFNQHTRG VWANNMCYNL HLLTGKISTP GSGPFSLTGQ PSACGTAREV 430 440 450 460 470 480 GTFSHRLPAD MVVTNEKHRQ IAETTWQLPA GTIPEKVGLH AVAQDRALKD GTLNAYWVMC 490 500 510 520 530 540 NNNMQAGPNI NEERMPGWRD PRNFIVVSDP YPTISALSAD LILPTSMWVE KEGAYGNAER 550 560 570 580 590 600 RTQFWRQQVP SPGEAKSDLW QIVEFAKRFN VEEVWPAELV NQKPEYRGKN LYEVLFANDV 610 620 630 640 650 660 VSKYPLSEIP DDQLNDEARD FGFYIQKGLF EEYASFGRGH AHDLAPFDVY HQVRGLRWPV 670 680 690 700 710 720 VDGKETLWRY REGFDPFVPK GEEVRFYGKP DGKAVIFALP YEPAAESPDQ EYDLWLSTGR 730 740 750 760 770 780 VLEHWHTGSM TRRVPELHRA FPEAVLFIHP LDAKARGLHR GDKVKVISRR GEVISLVETR 790 800 810 820 830 GRNRPPRGLV YMPFFDAAQL VNNLTLDATD PLSKETDFKK CAVKLERVVA

References

[1]

"Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen." Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L. J. Bacteriol. 188:4453-4463(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Antiqua.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000308 Genomic DNA. Translation: ABG14194.1.
RefSeq	YP_652139.1. NC_008150.1.
3D structure databases
ProteinModelPortal	Q1C5S8.
SMR	Q1C5S8. Positions 38-827.
ModBase	Search...
Protein-protein interaction databases
STRING	360102.YPA_2229.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABG14194; ABG14194; YPA_2229.
GeneID	4118196.
KEGG	ypa:YPA_2229.
PATRIC	18583978. VBIYerPes1796_2800.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HOG000031441.
KO	K02567.
OMA	HWIKAAR.
ProtClustDB	PRK13532.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
HAMAP	MF_01630. Nitrate_reduct.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. Periplasm_NO3_reductase_lsu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. PF10518. TAT_signal. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_YERPA

Accession

Primary (citable) accession number: Q1C5S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	July 11, 2006
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program