Reviewed, UniProtKB/Swiss-Prot Q1C5E7 (PUR4_YERPA)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	YPA_2360

Organism

Yersinia pestis bv. Antiqua (strain Antiqua) [Complete proteome] [HAMAP]

Taxonomic identifier

360102 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	1296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm. HAMAP MF_00419
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1296	1296	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000264602
Regions
	Domain	1043 – 1296	254	Glutamine amidotransferase type-1
	Nucleotide binding	306 – 317	12	ATP Potential
Sites
	Active site	1136	1	Nucleophile By similarity
	Active site	1261	1	By similarity
	Active site	1263	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1C5E7-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: 338EB52821A8888A	FASTA	1,296	142,048
	10 20 30 40 50 60 MEILRGSPAL SAFRITKLLS RCQDAHLLVS DIYAEYVHFA DVSAPLSADE HARLQRLLQY 70 80 90 100 110 120 GPSLPEHPPA GRLLLVTPRP GTISPWSSKA TDIAHNCGLS QILRLERGLA FSIQGPDLNE 130 140 150 160 170 180 SQWKQLAALL HDRMMEAVFT DLQQAEQLFS HHQPAPVQRV DILGQGRSAL EQANIKLGLA 190 200 210 220 230 240 LAQDEIDYLL TAFTGLGRNP TDIELYMFAQ ANSEHCRHKI FNADWVIDGV VQPKTLFKMI 250 260 270 280 290 300 KNTFEHTPDY VLSAYKDNAA VMEGSQVGRF YATAEKGIYD YHQEEAHILM KVETHNHPTA 310 320 330 340 350 360 ISPWPGAATG SGGEIRDEGA TGRGAKPKAG LVGFSVSNLR IPGFEQPWEE NFGKPDRIVT 370 380 390 400 410 420 ALDIMTEGPL GGAAFNNEFG RPALLGYFRT YEERVNSHNG IELRGYHKPI MLAGGLGNIR 430 440 450 460 470 480 ADHVQKGEIT VGAKLVVLGG PSMNIGLGGG AASSMASGQS DADLDFASVQ RDNPEMERRC 490 500 510 520 530 540 QEVIDRCWQL GEYNPILFIH DVGAGGLSNA MPELVNDGGR GGRFELRDIL NDEPGMSPLE 550 560 570 580 590 600 VWCNESQERY VLAVAPAQMA LFDEICRRER APYAVIGEAT EEKHLLLNDR HFGNQPIDMP 610 620 630 640 650 660 LDVLLGKTPK MLRDVTRLQA KGDALQRADI SLAEAVKRIM HLPAVAEKTF LITIGDRTVT 670 680 690 700 710 720 GMVTRDQMVG PWQIPVADCA VTSASLDSYY GEAMSLGERA PVALLDFAAS ARLAVGEALT 730 740 750 760 770 780 NIAATQIGEL KRIKLSANWM SAAGHPGEDA GLYDAVRAVG EELCPALEIT IPVGKDSMSM 790 800 810 820 830 840 KTRWQEGHEQ REMTSPLSLV ITAFARIEDV RRTVTPQLRT DKGDNALLLI DLGAGHNALG 850 860 870 880 890 900 ATALTQVYRQ LGDKPADVRN VQQLAGFFNA MQRLVADQHL LAYHDRSDGG LLVTLAEMAF 910 920 930 940 950 960 AGHCGVTVDI QSLGNDALAA LFNEELGAVI QVRAEQRADV EKLLADHGLA NCVHYLGRAV 970 980 990 1000 1010 1020 AGDTFDIRSG TDVVYSEKRS TLRLWWAETS WQMQRLRDNP DCADQEHQAK QDESDPGLNV 1030 1040 1050 1060 1070 1080 KLTFDPAEDI AAPFILKQAR PKVAVLREQG VNSHVEMAAA FHRAGFDAVD VHMSDLLAGR 1090 1100 1110 1120 1130 1140 TDLQSFQTLV ACGGFSYGDV LGAGEGWAKS ILFNDRVRDE FEAFFHRPTT LALGVCNGCQ 1150 1160 1170 1180 1190 1200 MMSNLRELIP GAEHWPRFVR NLSDSFEARF SLVEVASSPS LFMQDMVGSR MPIAVSHGEG 1210 1220 1230 1240 1250 1260 QVEVRDAAHL AALEQSHLVA LRFVNNHGVV TEQYPANPNG SANGITAVTS VSGRATVMMP 1270 1280 1290 HPERVFRTVS NSWHPEEWGE DSPWMRMFRN ARKQLG

References

[1]

"Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516: evidence of gene reduction in an emerging pathogen." Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M., Worsham P., Chu M.C., Andersen G.L. J. Bacteriol. 188:4453-4463(2006) [PubMed: 16740952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000308 Genomic DNA. Translation: ABG14325.1. Different initiation.
RefSeq	YP_652270.1.
3D structure databases
SMR	Q1C5E7. Positions 1-1296.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1C5E7.
Genome annotation databases
GeneID	4121241.
GenomeReviews	Gene locus YPA_2360 in contig CP000308_GR.
KEGG	ypa:YPA_2360.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1C5E7.
Enzyme and pathway databases
BioCyc	YPES360102:YPA_2360-MON.
Family and domain databases
HAMAP	MF_00419. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_YERPA

Accession

Primary (citable) accession number: Q1C5E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	September 1, 2009
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)