Q1BY75 (RNPH_BURCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	Bcen_0518

Organism

Burkholderia cenocepacia (strain AU 1054) [Complete proteome] [HAMAP]

Taxonomic identifier

331271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Ribonuclease PH HAMAP MF_00564		PRO_1000024783

Sequences

	Sequence		Length	Mass (Da)
	Q1BY75 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 40F5CC5E3261369B	FASTA	246	26,227
	10 20 30 40 50 60 MTSSVSRPSG RRADELRKVA LTRHYTKHAE GSVLVEFGDT KVLCTASVAE RVPEFLRERG 70 80 90 100 110 120 QGWLTAEYGM LPRATHTRSD REAARGKQTG RTQEIQRLIG RALRAVFDLE ALGPRTIHID 130 140 150 160 170 180 CDVIQADGGT RTASITGAFV AAHDAVSTLI AAGKLARSPI TDHVAAISVG VYEGAPVLDL 190 200 210 220 230 240 DYAEDSRCDT DMNVVMTGAG GFVEVQGTAE GVPFSRAEMN ALLDLAQGGI AELVQLQKDV LGASHA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia AU 1054." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., LiPuma J.J., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AU 1054.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000378 Genomic DNA. Translation: ABF75430.1.
RefSeq	YP_620403.1. NC_008060.1.
3D structure databases
ProteinModelPortal	Q1BY75.
SMR	Q1BY75. Positions 5-242.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1BY75.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4093553.
GenomeReviews	Gene locus Bcen_0518 in contig CP000378_GR.
KEGG	bcn:Bcen_0518.
PATRIC	19044499. VBIBurCen11237_0528.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
HOGENOM	HBG737187.
OMA	MLPRATG.
PhylomeDB	Q1BY75.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	BCEN331271:BCEN_0518-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_BURCA

Accession

Primary (citable) accession number: Q1BY75

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 11, 2006
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program