Reviewed, UniProtKB/Swiss-Prot Q1BWM4 (PYRD_BURCA)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	Bcen_1074

Organism

Burkholderia cenocepacia (strain AU 1054) [Complete proteome] [HAMAP]

Taxonomic identifier

331271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O(2) = orotate + H(2)O(2).
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane proteinBy similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Dihydroorotate dehydrogenase		PRO_0000336457
Sites
	Active site	178	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1BWM4-1 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 48BBC0EDFFFC0917	FASTA	345	36,756
	10 20 30 40 50 60 MFSSLYPLAR ASLFKMDAED AHHLTLRALG AAGRTGLACA LSARVPDAPR TVMGLTFRNP 70 80 90 100 110 120 VGLAAGLDKD GAAIDGLAAL GFGFIEVGTV TPRPQPGNPR PRMFRLPQAE ALINRMGFNN 130 140 150 160 170 180 HGVDQFVKNV QAARYRGILG LNIGKNADTP IERAAEDYLY CLERVYPFAS YVTINISSPN 190 200 210 220 230 240 TKNLRQLQGA GELDALLAAL KDKQQRLADL HGKLVPLALK IAPDLDDEQV KEIGDTLLRH 250 260 270 280 290 300 KIEAVIATNT TLSRAAVQGL PHADEAGGLS GRPVFDASNE VIRKLHAEVG NDVPIIGVGG 310 320 330 340 IFSGEDAHAK LAAGAALVQL YTGFIYRGPA LVSECVKAIA RERSA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia AU 1054." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., LiPuma J.J., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000378 Genomic DNA. Translation: ABF75981.1. Different initiation.
RefSeq	YP_620954.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4093165.
GenomeReviews	Gene locus Bcen_1074 in contig CP000378_GR.
KEGG	bcn:Bcen_1074.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1BWM4.
Enzyme and pathway databases
BioCyc	BCEN331271:BCEN_1074-MON.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. DHO_DHase_1_2. IPR005719. DHO_DHase_2. IPR001295. Dihydroorotate_DHase_core. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_BURCA

Accession

Primary (citable) accession number: Q1BWM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	November 25, 2008
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)