Reviewed, UniProtKB/Swiss-Prot Q1BUH6 (SYM_BURCA)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	Bcen_1826

Organism

Burkholderia cenocepacia (strain AU 1054) [Complete proteome] [HAMAP]

Taxonomic identifier

331271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 718	718	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000331787
Regions
	Domain	612 – 718	107	tRNA-binding
	Motif	27 – 37	11	"HIGH" region HAMAP MF_00098
	Motif	348 – 352	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	158	1	Zinc By similarity
	Metal binding	161	1	Zinc By similarity
	Metal binding	171	1	Zinc By similarity
	Metal binding	174	1	Zinc By similarity
	Binding site	351	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1BUH6-1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: F355F74003839A3D	FASTA	718	79,227
	10 20 30 40 50 60 MSASDLTSVQ AAAPQGSRQI LVTSALPYAN GQIHIGHLVE YIQTDIWVRT LRMHGHEVYY 70 80 90 100 110 120 IGADDTHGTP IMLRAEKEGL TPKQLIDRVW TEHKRDFDSF GVSFDNFYTT DSEENRVLSE 130 140 150 160 170 180 NIYLALQEAG LIAEREIEQA YDPVKEMFLP DRFIKGECPK CHAKDQYGDS CEVCGSTYLP 190 200 210 220 230 240 TELLNPYSVV SGATPVRKTS KHYFFRLSDP RCESFLREWV SGLAQPEATN KMREWLGDAG 250 260 270 280 290 300 ESKLADWDIS RDAPYFGFEI PGAPGKYFYV WLDAPVGYYA SFKNLCERNG IDFDAWIRPG 310 320 330 340 350 360 SKAEQYHFIG KDILYFHTLF WPAMLEFSGH RTPTNVFAHG FLTVDGAKMS KSRGTFITAQ 370 380 390 400 410 420 SYIDTGLNPE WLRYYFAAKL NATMEDIDLN LDDFQARVNS DLVGKYVNIA SRAAGFLIKR 430 440 450 460 470 480 FDGRVQDSAM NHPLVAKLRD AIPQIAASYE AREYGRALRH TMELADEVNA YVDGAKPWDL 490 500 510 520 530 540 AKDPANAVAL HETCSVSLEA FRLLSLALKP VMPRVAVAVE AFFGIAPLAW ADAAKPLSSA 550 560 570 580 590 600 QPIKAYQHLM TRVDAKQIEA LLAANRDSLQ AEAAGAAAAG ANAAKDAKSN AKASAKPAAV 610 620 630 640 650 660 NGADDAPISI DDFAKIDLRI AKIVACQAVE GSDKLLQLTL DVGEEKTRNV FSGIKSAYQP 670 680 690 700 710 EQLVGKLTVM VANLAPRKMK FGLSEGMVLA ASATDEKAEP GLYILEPHSG AKPGMRVK

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia AU 1054." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., LiPuma J.J., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000378 Genomic DNA. Translation: ABF76729.1. Different initiation.
RefSeq	YP_621702.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1BUH6.
Genome annotation databases
GeneID	4093371.
GenomeReviews	Gene locus Bcen_1826 in contig CP000378_GR.
KEGG	bcn:Bcen_1826.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1BUH6.
Enzyme and pathway databases
BioCyc	BCEN331271:BCEN_1826-MON.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BURCA

Accession

Primary (citable) accession number: Q1BUH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	September 1, 2009
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)