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Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene

aspS

Organism
Burkholderia cenocepacia (strain AU 1054)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei32Important for tRNA non-discriminationUniRule annotation1
Sitei83Important for tRNA non-discriminationUniRule annotation1
Binding sitei174AspartateUniRule annotation1
Binding sitei220AspartateUniRule annotation1
Binding sitei229ATPUniRule annotation1
Binding sitei457AspartateUniRule annotation1
Binding sitei491ATPUniRule annotation1
Binding sitei498AspartateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi220 – 222ATPUniRule annotation3
Nucleotide bindingi543 – 546ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Non-discriminating aspartyl-tRNA synthetaseUniRule annotation
Short name:
ND-AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotation
Ordered Locus Names:Bcen_2113
OrganismiBurkholderia cenocepacia (strain AU 1054)
Taxonomic identifieri331271 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000002400 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000066441 – 600Aspartate--tRNA(Asp/Asn) ligaseAdd BLAST600

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ1BTP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni198 – 201AspartateUniRule annotation4

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000275160.
KOiK01876.
OMAiYQLDVEM.

Family and domain databases

Gene3Di3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth_type1. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_bac/mit.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 2 hits.
PTHR22594:SF5. PTHR22594:SF5. 2 hits.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1BTP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMRTEYCGL VTEHLLGQTV SLCGWVQRRR DHGGVIFIDL RDREGLVQVV
60 70 80 90 100
CDPDRAEMFA TAEGVRNEFC VQIKGLVRNR PDGTVNAGLK SGRIEVLCHE
110 120 130 140 150
LNVLNASVTP PFQLDDDNLS ETTRLTHRVL DLRRPQMQHN LRLRYRVAIE
160 170 180 190 200
ARKYLDEQGF IDIETPMLTK STPEGARDYL VPSRVNAGQF FALPQSPQLF
210 220 230 240 250
KQLLMVANFD RYYQITKCFR DEDLRADRQP EFTQIDCETS FLGEQEIRDL
260 270 280 290 300
FEDMIRHIFK TTIDVELDAK FPVMPYSEAM ARFGSDKPDL RVQLEFTELT
310 320 330 340 350
DAMKDVDFKV FSTPANAKDG RVAALRVPKG GELSRGDIDG YTEFVRIYGA
360 370 380 390 400
KGLAWIKVNE KAKGRDGLQS PIVKNLHDAS IAAILERTGA EDGDIIFFAA
410 420 430 440 450
DRAKVVNDSL GALRLKIGHS EFGKANGLVQ AGWKPLWVVD FPMFEYDDED
460 470 480 490 500
ARYVAAHHPF TSPKDEHLEY LETDPGRCLA KAYDMVLNGW EIGGGSVRIH
510 520 530 540 550
REEVQSKVFR ALKIGAEEAQ LKFGFLLDAL QYGAPPHGGI AFGLDRIVTM
560 570 580 590 600
MAGADSIRDV IAFPKTQRAQ DLLTQAPSPV DERQLRELHI RLRQPEQPKA
Length:600
Mass (Da):67,757
Last modified:July 11, 2006 - v1
Checksum:i4FDAD415B483C6AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000378 Genomic DNA. Translation: ABF77014.1.
RefSeqiWP_011546184.1. NC_008060.1.

Genome annotation databases

EnsemblBacteriaiABF77014; ABF77014; Bcen_2113.
KEGGibcn:Bcen_2113.
PATRICi19047917. VBIBurCen11237_2202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000378 Genomic DNA. Translation: ABF77014.1.
RefSeqiWP_011546184.1. NC_008060.1.

3D structure databases

ProteinModelPortaliQ1BTP1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF77014; ABF77014; Bcen_2113.
KEGGibcn:Bcen_2113.
PATRICi19047917. VBIBurCen11237_2202.

Phylogenomic databases

HOGENOMiHOG000275160.
KOiK01876.
OMAiYQLDVEM.

Family and domain databases

Gene3Di3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth_type1. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_bac/mit.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 2 hits.
PTHR22594:SF5. PTHR22594:SF5. 2 hits.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYDND_BURCA
AccessioniPrimary (citable) accession number: Q1BTP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 11, 2006
Last modified: November 30, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.