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Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene

aspS

Organism
Burkholderia cenocepacia (strain AU 1054)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei32 – 321Important for tRNA non-discriminationUniRule annotation
Sitei83 – 831Important for tRNA non-discriminationUniRule annotation
Binding sitei174 – 1741AspartateUniRule annotation
Binding sitei220 – 2201AspartateUniRule annotation
Binding sitei229 – 2291ATPUniRule annotation
Binding sitei457 – 4571AspartateUniRule annotation
Binding sitei491 – 4911ATPUniRule annotation
Binding sitei498 – 4981AspartateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi220 – 2223ATPUniRule annotation
Nucleotide bindingi543 – 5464ATPUniRule annotation

GO - Molecular functioni

  1. aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
  2. aspartate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. ATP binding Source: UniProtKB-HAMAP
  4. nucleic acid binding Source: InterPro

GO - Biological processi

  1. aspartyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBCEN331271:GHKX-2159-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Non-discriminating aspartyl-tRNA synthetaseUniRule annotation
Short name:
ND-AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotation
Ordered Locus Names:Bcen_2113
OrganismiBurkholderia cenocepacia (strain AU 1054)
Taxonomic identifieri331271 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000002400: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600Aspartate--tRNA(Asp/Asn) ligasePRO_1000006644Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi331271.Bcen_2113.

Structurei

3D structure databases

ProteinModelPortaliQ1BTP1.
SMRiQ1BTP1. Positions 3-593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni198 – 2014AspartateUniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0173.
HOGENOMiHOG000275160.
KOiK01876.
OMAiYQLDVEM.
OrthoDBiEOG68Q0NX.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1BTP1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSMRTEYCGL VTEHLLGQTV SLCGWVQRRR DHGGVIFIDL RDREGLVQVV
60 70 80 90 100
CDPDRAEMFA TAEGVRNEFC VQIKGLVRNR PDGTVNAGLK SGRIEVLCHE
110 120 130 140 150
LNVLNASVTP PFQLDDDNLS ETTRLTHRVL DLRRPQMQHN LRLRYRVAIE
160 170 180 190 200
ARKYLDEQGF IDIETPMLTK STPEGARDYL VPSRVNAGQF FALPQSPQLF
210 220 230 240 250
KQLLMVANFD RYYQITKCFR DEDLRADRQP EFTQIDCETS FLGEQEIRDL
260 270 280 290 300
FEDMIRHIFK TTIDVELDAK FPVMPYSEAM ARFGSDKPDL RVQLEFTELT
310 320 330 340 350
DAMKDVDFKV FSTPANAKDG RVAALRVPKG GELSRGDIDG YTEFVRIYGA
360 370 380 390 400
KGLAWIKVNE KAKGRDGLQS PIVKNLHDAS IAAILERTGA EDGDIIFFAA
410 420 430 440 450
DRAKVVNDSL GALRLKIGHS EFGKANGLVQ AGWKPLWVVD FPMFEYDDED
460 470 480 490 500
ARYVAAHHPF TSPKDEHLEY LETDPGRCLA KAYDMVLNGW EIGGGSVRIH
510 520 530 540 550
REEVQSKVFR ALKIGAEEAQ LKFGFLLDAL QYGAPPHGGI AFGLDRIVTM
560 570 580 590 600
MAGADSIRDV IAFPKTQRAQ DLLTQAPSPV DERQLRELHI RLRQPEQPKA
Length:600
Mass (Da):67,757
Last modified:July 11, 2006 - v1
Checksum:i4FDAD415B483C6AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000378 Genomic DNA. Translation: ABF77014.1.
RefSeqiYP_621987.1. NC_008060.1.

Genome annotation databases

EnsemblBacteriaiABF77014; ABF77014; Bcen_2113.
GeneIDi4091123.
KEGGibcn:Bcen_2113.
PATRICi19047917. VBIBurCen11237_2202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000378 Genomic DNA. Translation: ABF77014.1.
RefSeqiYP_621987.1. NC_008060.1.

3D structure databases

ProteinModelPortaliQ1BTP1.
SMRiQ1BTP1. Positions 3-593.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi331271.Bcen_2113.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF77014; ABF77014; Bcen_2113.
GeneIDi4091123.
KEGGibcn:Bcen_2113.
PATRICi19047917. VBIBurCen11237_2202.

Phylogenomic databases

eggNOGiCOG0173.
HOGENOMiHOG000275160.
KOiK01876.
OMAiYQLDVEM.
OrthoDBiEOG68Q0NX.

Enzyme and pathway databases

BioCyciBCEN331271:GHKX-2159-MONOMER.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AU 1054.

Entry informationi

Entry nameiSYDND_BURCA
AccessioniPrimary (citable) accession number: Q1BTP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 11, 2006
Last modified: January 7, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.