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Q1BTP1

- SYDND_BURCA

UniProt

Q1BTP1 - SYDND_BURCA

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Protein
Aspartate--tRNA(Asp/Asn) ligase
Gene
aspS, Bcen_2113
Organism
Burkholderia cenocepacia (strain AU 1054)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn) By similarity.UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei32 – 321Important for tRNA non-discrimination By similarity
Sitei83 – 831Important for tRNA non-discrimination By similarity
Binding sitei174 – 1741Aspartate By similarity
Binding sitei220 – 2201Aspartate By similarity
Binding sitei229 – 2291ATP By similarity
Binding sitei457 – 4571Aspartate By similarity
Binding sitei491 – 4911ATP By similarity
Binding sitei498 – 4981Aspartate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi220 – 2223ATP By similarity
Nucleotide bindingi543 – 5464ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aspartate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
  4. nucleic acid binding Source: InterPro

GO - Biological processi

  1. aspartyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBCEN331271:GHKX-2159-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA(Asp/Asn) ligase (EC:6.1.1.23)
Alternative name(s):
Aspartyl-tRNA synthetase
Short name:
AspRS
Non-discriminating aspartyl-tRNA synthetase
Short name:
ND-AspRS
Gene namesi
Name:aspS
Ordered Locus Names:Bcen_2113
OrganismiBurkholderia cenocepacia (strain AU 1054)
Taxonomic identifieri331271 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000002400: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation
PRO_1000006644Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi331271.Bcen_2113.

Structurei

3D structure databases

ProteinModelPortaliQ1BTP1.
SMRiQ1BTP1. Positions 3-593.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni198 – 2014Aspartate By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0173.
HOGENOMiHOG000275160.
KOiK01876.
OMAiSLSRKQI.
OrthoDBiEOG68Q0NX.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1BTP1-1 [UniParc]FASTAAdd to Basket

« Hide

MSMRTEYCGL VTEHLLGQTV SLCGWVQRRR DHGGVIFIDL RDREGLVQVV    50
CDPDRAEMFA TAEGVRNEFC VQIKGLVRNR PDGTVNAGLK SGRIEVLCHE 100
LNVLNASVTP PFQLDDDNLS ETTRLTHRVL DLRRPQMQHN LRLRYRVAIE 150
ARKYLDEQGF IDIETPMLTK STPEGARDYL VPSRVNAGQF FALPQSPQLF 200
KQLLMVANFD RYYQITKCFR DEDLRADRQP EFTQIDCETS FLGEQEIRDL 250
FEDMIRHIFK TTIDVELDAK FPVMPYSEAM ARFGSDKPDL RVQLEFTELT 300
DAMKDVDFKV FSTPANAKDG RVAALRVPKG GELSRGDIDG YTEFVRIYGA 350
KGLAWIKVNE KAKGRDGLQS PIVKNLHDAS IAAILERTGA EDGDIIFFAA 400
DRAKVVNDSL GALRLKIGHS EFGKANGLVQ AGWKPLWVVD FPMFEYDDED 450
ARYVAAHHPF TSPKDEHLEY LETDPGRCLA KAYDMVLNGW EIGGGSVRIH 500
REEVQSKVFR ALKIGAEEAQ LKFGFLLDAL QYGAPPHGGI AFGLDRIVTM 550
MAGADSIRDV IAFPKTQRAQ DLLTQAPSPV DERQLRELHI RLRQPEQPKA 600
Length:600
Mass (Da):67,757
Last modified:July 11, 2006 - v1
Checksum:i4FDAD415B483C6AF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000378 Genomic DNA. Translation: ABF77014.1.
RefSeqiYP_621987.1. NC_008060.1.

Genome annotation databases

EnsemblBacteriaiABF77014; ABF77014; Bcen_2113.
GeneIDi4091123.
KEGGibcn:Bcen_2113.
PATRICi19047917. VBIBurCen11237_2202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000378 Genomic DNA. Translation: ABF77014.1 .
RefSeqi YP_621987.1. NC_008060.1.

3D structure databases

ProteinModelPortali Q1BTP1.
SMRi Q1BTP1. Positions 3-593.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 331271.Bcen_2113.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABF77014 ; ABF77014 ; Bcen_2113 .
GeneIDi 4091123.
KEGGi bcn:Bcen_2113.
PATRICi 19047917. VBIBurCen11237_2202.

Phylogenomic databases

eggNOGi COG0173.
HOGENOMi HOG000275160.
KOi K01876.
OMAi SLSRKQI.
OrthoDBi EOG68Q0NX.

Enzyme and pathway databases

BioCyci BCEN331271:GHKX-2159-MONOMER.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPi MF_00044. Asp_tRNA_synth.
InterProi IPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view ]
PANTHERi PTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
Pfami PF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view ]
PRINTSi PR01042. TRNASYNTHASP.
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsi TIGR00459. aspS_bact. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AU 1054.

Entry informationi

Entry nameiSYDND_BURCA
AccessioniPrimary (citable) accession number: Q1BTP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 11, 2006
Last modified: July 9, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi