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Q1BTP1

- SYDND_BURCA

UniProt

Q1BTP1 - SYDND_BURCA

Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene

aspS

Organism
Burkholderia cenocepacia (strain AU 1054)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (11 Jul 2006)
      Previous versions | rss
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    Functioni

    Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation

    Catalytic activityi

    ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei32 – 321Important for tRNA non-discriminationUniRule annotation
    Sitei83 – 831Important for tRNA non-discriminationUniRule annotation
    Binding sitei174 – 1741AspartateUniRule annotation
    Binding sitei220 – 2201AspartateUniRule annotation
    Binding sitei229 – 2291ATPUniRule annotation
    Binding sitei457 – 4571AspartateUniRule annotation
    Binding sitei491 – 4911ATPUniRule annotation
    Binding sitei498 – 4981AspartateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi220 – 2223ATPUniRule annotation
    Nucleotide bindingi543 – 5464ATPUniRule annotation

    GO - Molecular functioni

    1. aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
    2. aspartate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. ATP binding Source: UniProtKB-HAMAP
    4. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. aspartyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBCEN331271:GHKX-2159-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)
    Alternative name(s):
    Aspartyl-tRNA synthetaseUniRule annotation
    Short name:
    AspRSUniRule annotation
    Non-discriminating aspartyl-tRNA synthetaseUniRule annotation
    Short name:
    ND-AspRSUniRule annotation
    Gene namesi
    Name:aspSUniRule annotation
    Ordered Locus Names:Bcen_2113
    OrganismiBurkholderia cenocepacia (strain AU 1054)
    Taxonomic identifieri331271 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
    ProteomesiUP000002400: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 600600Aspartate--tRNA(Asp/Asn) ligasePRO_1000006644Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi331271.Bcen_2113.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1BTP1.
    SMRiQ1BTP1. Positions 3-593.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni198 – 2014AspartateUniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0173.
    HOGENOMiHOG000275160.
    KOiK01876.
    OMAiSLSRKQI.
    OrthoDBiEOG68Q0NX.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.1360.30. 1 hit.
    HAMAPiMF_00044. Asp_tRNA_synth.
    InterProiIPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR004115. GAD-like.
    IPR029351. GAD_dom.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view]
    PANTHERiPTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF5. PTHR22594:SF5. 1 hit.
    PfamiPF02938. GAD. 1 hit.
    PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view]
    PRINTSiPR01042. TRNASYNTHASP.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF55261. SSF55261. 1 hit.
    TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1BTP1-1 [UniParc]FASTAAdd to Basket

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    MSMRTEYCGL VTEHLLGQTV SLCGWVQRRR DHGGVIFIDL RDREGLVQVV    50
    CDPDRAEMFA TAEGVRNEFC VQIKGLVRNR PDGTVNAGLK SGRIEVLCHE 100
    LNVLNASVTP PFQLDDDNLS ETTRLTHRVL DLRRPQMQHN LRLRYRVAIE 150
    ARKYLDEQGF IDIETPMLTK STPEGARDYL VPSRVNAGQF FALPQSPQLF 200
    KQLLMVANFD RYYQITKCFR DEDLRADRQP EFTQIDCETS FLGEQEIRDL 250
    FEDMIRHIFK TTIDVELDAK FPVMPYSEAM ARFGSDKPDL RVQLEFTELT 300
    DAMKDVDFKV FSTPANAKDG RVAALRVPKG GELSRGDIDG YTEFVRIYGA 350
    KGLAWIKVNE KAKGRDGLQS PIVKNLHDAS IAAILERTGA EDGDIIFFAA 400
    DRAKVVNDSL GALRLKIGHS EFGKANGLVQ AGWKPLWVVD FPMFEYDDED 450
    ARYVAAHHPF TSPKDEHLEY LETDPGRCLA KAYDMVLNGW EIGGGSVRIH 500
    REEVQSKVFR ALKIGAEEAQ LKFGFLLDAL QYGAPPHGGI AFGLDRIVTM 550
    MAGADSIRDV IAFPKTQRAQ DLLTQAPSPV DERQLRELHI RLRQPEQPKA 600
    Length:600
    Mass (Da):67,757
    Last modified:July 11, 2006 - v1
    Checksum:i4FDAD415B483C6AF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000378 Genomic DNA. Translation: ABF77014.1.
    RefSeqiYP_621987.1. NC_008060.1.

    Genome annotation databases

    EnsemblBacteriaiABF77014; ABF77014; Bcen_2113.
    GeneIDi4091123.
    KEGGibcn:Bcen_2113.
    PATRICi19047917. VBIBurCen11237_2202.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000378 Genomic DNA. Translation: ABF77014.1 .
    RefSeqi YP_621987.1. NC_008060.1.

    3D structure databases

    ProteinModelPortali Q1BTP1.
    SMRi Q1BTP1. Positions 3-593.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 331271.Bcen_2113.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABF77014 ; ABF77014 ; Bcen_2113 .
    GeneIDi 4091123.
    KEGGi bcn:Bcen_2113.
    PATRICi 19047917. VBIBurCen11237_2202.

    Phylogenomic databases

    eggNOGi COG0173.
    HOGENOMi HOG000275160.
    KOi K01876.
    OMAi SLSRKQI.
    OrthoDBi EOG68Q0NX.

    Enzyme and pathway databases

    BioCyci BCEN331271:GHKX-2159-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.1360.30. 1 hit.
    HAMAPi MF_00044. Asp_tRNA_synth.
    InterProi IPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR004115. GAD-like.
    IPR029351. GAD_dom.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view ]
    PANTHERi PTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF5. PTHR22594:SF5. 1 hit.
    Pfami PF02938. GAD. 1 hit.
    PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view ]
    PRINTSi PR01042. TRNASYNTHASP.
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF55261. SSF55261. 1 hit.
    TIGRFAMsi TIGR00459. aspS_bact. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AU 1054.

    Entry informationi

    Entry nameiSYDND_BURCA
    AccessioniPrimary (citable) accession number: Q1BTP1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: July 11, 2006
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3