Reviewed, UniProtKB/Swiss-Prot Q1BTG7 (ISPE_BURCA)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Bcen_2187

Organism

Burkholderia cenocepacia (strain AU 1054) [Complete proteome] [HAMAP]

Taxonomic identifier

331271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity.
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000007817
Regions
	Nucleotide binding	99 – 109	11	ATP Potential
Sites
	Active site	16	1	By similarity
	Active site	141	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1BTG7-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: EF09D3C9E289DB7C	FASTA	293	32,085
	10 20 30 40 50 60 MTDSTRSLRN CLAPAKLNLF LHITGRRPNG YHDLQSVFQL LNWGDTLHFT RRDDGRVARV 70 80 90 100 110 120 TDVPGVPEES DLVVRAANLL KAHTDTAHGV DIEIDKILPM GAGLGGGSSD AATTLLALNR 130 140 150 160 170 180 LWQLDLPRAE LQSLAVKLGA DVPFFIFGKN AFAEGIGEEL AEVELPTRWF LVVTPRVHVP 190 200 210 220 230 240 TAEIFSDELL TRDTKPVTIA DFLAQQTSDA EWPDSFGRND MQEVVTRKYA EVAQVVKWLY 250 260 270 280 290 DVTPARMTGS GASVFAAFHS KHEAEAAKAK LPASWNGAVA ESLNEHPLFA FAS

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia AU 1054." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., LiPuma J.J., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000378 Genomic DNA. Translation: ABF77088.1.
RefSeq	YP_622061.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1BTG7.
Genome annotation databases
GeneID	4092119.
GenomeReviews	Gene locus Bcen_2187 in contig CP000378_GR.
KEGG	bcn:Bcen_2187.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1BTG7.
OMA	PMGGGIG.
Enzyme and pathway databases
BioCyc	BCEN331271:BCEN_2187-MON.
Family and domain databases
HAMAP	MF_00061. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_BURCA

Accession

Primary (citable) accession number: Q1BTG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 11, 2006
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)