Q1BRU5 (EFG2_BURCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G 2
Short name=EF-G 2

Gene names

Name:	fusA2
Ordered Locus Names:	Bcen_2762

Organism

Burkholderia cenocepacia (strain AU 1054) [Complete proteome] [HAMAP]

Taxonomic identifier

331271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	700 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 700	700	Elongation factor G 2 HAMAP MF_00054_B		PRO_0000263434
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	88 – 92	5	GTP By similarity
	Nucleotide binding	142 – 145	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1BRU5 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 32B2EE2883462AB2	FASTA	700	77,506
	10 20 30 40 50 60 MARKTPIERY RNIGISAHID AGKTTTTERI LFYTGVNHKI GEVHDGAATM DWMEQEQERG 70 80 90 100 110 120 ITITSAATTA FWKGMGGNYP EHRINIIDTP GHVDFTIEVE RSMRVLDGAC MVYCAVGGVQ 130 140 150 160 170 180 PQSETVWRQA NKYKVPRLAF VNKMDRTGAN FFKVYDQLRL RLKANPVPVV VPIGAEENFK 190 200 210 220 230 240 GVVDLIKMKA IIWDEASQGT KFDYVDIPAE LAETCKEWRE KMVEVAAEAS EDLMNKYLEE 250 260 270 280 290 300 GDLPEADIVK ALRDRTIACE IQPMLCGTAF KNKGVQRMLD AVIDFLPSPV DIPPVKGELE 310 320 330 340 350 360 NGEEAERKAS DEEKFSSLAF KIMTDPFVGQ LIFFRVYSGV VNSGDTLLNS TKGKKERLGR 370 380 390 400 410 420 ILQMHANQRE EIKEVRAGDI AAAVGLKEAT TGDTLCDPAN PIVLERMVFP EPVISQAVEP 430 440 450 460 470 480 KTKADQEKMG LALNRLAQED PSFRVQTDEE SGQTIISGMG ELHLEILVDR MKREFGVEAT 490 500 510 520 530 540 VGKPQVAYRE TIRSTAKDVD GKFVKQSGGR GQYGHAVITL EPNEQGKGYE FFDEIKGGVI 550 560 570 580 590 600 PREYIPAVDK GIQDTLKSGV LAGFPVVDVK VHLTFGSYHD VDSNENAFRM AGSMAFKEAM 610 620 630 640 650 660 RKANPVVLEP MMAVEVETPE DYMGNVMGDL SGRRGIVQGM EDMVGGGKIV RAEVPLSEMF 670 680 690 700 GYSTSLRSLA QGRATYTMEF KHYAEAPRNV AEAIISAKSK

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia AU 1054." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., LiPuma J.J., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AU 1054.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000378 Genomic DNA. Translation: ABF77660.1.
RefSeq	YP_622633.1. NC_008060.1.
3D structure databases
ProteinModelPortal	Q1BRU5.
SMR	Q1BRU5. Positions 1-695.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1BRU5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4092461.
GenomeReviews	Gene locus Bcen_2762 in contig CP000378_GR.
KEGG	bcn:Bcen_2762.
PATRIC	19049316. VBIBurCen11237_2892.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0480.
HOGENOM	HBG737692.
OMA	CNEWREK.
PhylomeDB	Q1BRU5.
ProtClustDB	PRK00007.
Enzyme and pathway databases
BioCyc	BCEN331271:BCEN_2762-MONOMER.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG2_BURCA

Accession

Primary (citable) accession number: Q1BRU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	July 11, 2006
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program