Reviewed, UniProtKB/Swiss-Prot Q1BRL2 (CHEB1_BURCA)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	Bcen_2846

Organism

Burkholderia cenocepacia (strain AU 1054) [Complete proteome] [HAMAP]

Taxonomic identifier

331271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000264264
Regions
	Domain	5 – 122	118	Response regulatory
	Domain	161 – 353	193	CheB-type methylesterase
Sites
	Active site	173	1	By similarity
	Active site	199	1	By similarity
	Active site	295	1	By similarity
Amino acid modifications
	Modified residue	56	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1BRL2-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: CBF0F61D440BD2C1	FASTA	360	38,618
	10 20 30 40 50 60 MQKIKVLCVD DSALIRSLMT EIINSQPDMT VVATAPDPLV ARELIKQHNP DVLTLDVEMP 70 80 90 100 110 120 RMDGLDFLEK LMRLRPMPVV MVSSLTERGS EITLRALELG AVDFVTKPRV GIRDGMLDYA 130 140 150 160 170 180 EKLADKIRAA SRARVRQAPQ PQAAARSADS AAAAPMINNP LVSTEKLIII GASTGGTEAI 190 200 210 220 230 240 REVLTPLPPD APAVLIAQHM PPGFTKSFAQ RLNGLCRIAV KEAEHGERVL PGHAYIAPGH 250 260 270 280 290 300 AHLLLARSGA NYIAQLSDEP PVNRHRPSVD VLFRSAATHA GKNAIGVILT GMGRDGAAGL 310 320 330 340 350 360 LEMKRAGAHT FAQDEASCIV FGMPREAIAL GGADEVAPLA DMSRRVMARL ATMGDRVQRV

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia AU 1054." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., LiPuma J.J., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000378 Genomic DNA. Translation: ABF77743.1.
RefSeq	YP_622716.1.
3D structure databases
SMR	Q1BRL2. Positions 1-351.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1BRL2.
Genome annotation databases
GeneID	4090961.
GenomeReviews	Gene locus Bcen_2846 in contig CP000378_GR.
KEGG	bcn:Bcen_2846.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1BRL2.
OMA	IVIAQHI.
Enzyme and pathway databases
BioCyc	BCEN331271:BCEN_2846-MON.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_BURCA

Accession

Primary (citable) accession number: Q1BRL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	July 11, 2006
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)