Reviewed, UniProtKB/Swiss-Prot Q1BQP0 (KATG2_BURCA)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 2
      Short name=CP 2
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 2

Gene names

Name:	katG2
Ordered Locus Names:	Bcen_3169

Organism

Burkholderia cenocepacia (strain AU 1054) [Complete proteome] [HAMAP]

Taxonomic identifier

331271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Catalase-peroxidase 2 HAMAP MF_01961		PRO_0000354733
Sites
	Active site	92	1	Proton acceptor By similarity
	Metal binding	255	1	Iron (heme axial ligand) By similarity
	Site	88	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	91 ↔ 214		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-240) By similarity
	Cross-link	214 ↔ 240		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-91) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1BQP0-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 24FBBA5BA38AAB9C	FASTA	728	79,926
	10 20 30 40 50 60 MSNEGKCPFN HGKRNGTTNR DWWPNQLNLK ILHQHSSEAD PMDPGFDYAE AFNSLDLAAV 70 80 90 100 110 120 KADLRALMTA SQDWWPADFG HYGPFFVRMA WHSAGTYRTG DGRGGAGRGQ QRFAPLNSWP 130 140 150 160 170 180 DNVGLDKARR LIWPVKQKYG RKISWADLIV LTGNVALESM GFKTFGFAGG REDSWEPDED 190 200 210 220 230 240 VYWGMESTWL DDKRYSGDRQ LETPLAAVQM GLIYVNPEGP NGNPDPLASA RDIRETFARM 250 260 270 280 290 300 AMNDEETVAL IAGGHTFGKT HGAGDASHVG PEPEAAPLEQ MGLGWKSSFG SGKAGDAIGS 310 320 330 340 350 360 GLEVIWTSTP TQWSNNFFWN LFGYDWELTK SPAGAHQWQP KGGAGADSVP DPFEPGKRRV 370 380 390 400 410 420 PTMLTSDIAL RADPTYEKIS RRFFENPNEF AEAFARAWFK LTHRDMGPRV RYLGPEVPSE 430 440 450 460 470 480 ELLWQDPIPM PDHPQVDEQD VSALKAKVLA SGLSVSELVS TAWASASTFR GSDKRGGANG 490 500 510 520 530 540 ARVRLAPQKD WEVNQPAQLA TVLEVLGALQ VEFNRAATGG KQVSLADLIV IAGNAGVEQA 550 560 570 580 590 600 AAAAGVEITV PFTPGRGDAS AEQTDVDSMA VLEPIADGFR NYLKGAYTIP AEKLLIDKAQ 610 620 630 640 650 660 LLSLSAPEMT VLIGGLRVLG TNVGDSKHGV FTDRREVLTN DFFRNLLDMG TEWKPTSEAN 670 680 690 700 710 720 EAYEGRDRAT GELKWLASRV DLVFGSHSQL RALSEVYGSE DSQQKFVRDF VAAWTKVMNA DRFDIKHN

References

[1]

"Complete sequence of chromosome 2 of Burkholderia cenocepacia AU 1054." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., LiPuma J.J., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000379 Genomic DNA. Translation: ABF78065.1.
RefSeq	YP_623038.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4095370.
GenomeReviews	Gene locus Bcen_3169 in contig CP000379_GR.
KEGG	bcn:Bcen_3169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1BQP0.
OMA	Q1BQP0. NGWANSV.
Enzyme and pathway databases
BioCyc	BCEN331271:BCEN_3169-MON.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_BURCA

Accession

Primary (citable) accession number: Q1BQP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	July 11, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)