Q1BMF9 (CHEB2_BURCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 2
EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	Bcen_4310

Organism

Burkholderia cenocepacia (strain AU 1054) [Complete proteome] [HAMAP]

Taxonomic identifier

331271 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Chemotaxis response regulator protein-glutamate methylesterase 2 HAMAP MF_00099		PRO_0000264265
Regions
	Domain	2 – 120	119	Response regulatory
	Domain	134 – 334	201	CheB-type methylesterase
Sites
	Active site	157	1	By similarity
	Active site	184	1	By similarity
	Active site	277	1	By similarity
Amino acid modifications
	Modified residue	53	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1BMF9 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: BD6430F17EB27BD8	FASTA	334	34,982
	10 20 30 40 50 60 MNIGIVNDLP LAVEAMRRAI ARRPEHRVLW VATDGPQAVE LCAAQPPDIV LMDLIMPKFD 70 80 90 100 110 120 GIEATRRIMR PERPCAILIV TSCIGANAWR VFEAMGAGAL DAVDTPRLGD GAAGDTTKLL 130 140 150 160 170 180 LAKIDQIGRL LDAPGSSRLA GAAARGGAGP LIAIGASAGG PGALASILGN LPADFNAPIV 190 200 210 220 230 240 IVQHVDRAFA EGMAQWLDGQ TRLAVRVARE GDRPQPGVAL LAATDDHLRI TRAGTLEYTR 250 260 270 280 290 300 EPAATPYRPS VDVFFNSLTE HWPGRVIGVL LTGMGRDGAI GLKALRMKGY HTIAQDEATS 310 320 330 AVYGMPKAAA TLGAARAILP LGRIAGELAA LARI

References

[1]

"Complete sequence of chromosome 2 of Burkholderia cenocepacia AU 1054." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., LiPuma J.J., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AU 1054.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000379 Genomic DNA. Translation: ABF79196.1.
RefSeq	YP_624169.1. NC_008061.1.
3D structure databases
ProteinModelPortal	Q1BMF9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1BMF9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4094897.
GenomeReviews	Gene locus Bcen_4310 in contig CP000379_GR.
KEGG	bcn:Bcen_4310.
PATRIC	19052566. VBIBurCen11237_4492.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	RPCAILI.
ProtClustDB	PRK12555.
Enzyme and pathway databases
BioCyc	BCEN331271:BCEN_4310-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_BURCA

Accession

Primary (citable) accession number: Q1BMF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	July 11, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program