Q1BK24 (TDH_BURCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-threonine 3-dehydrogenase
EC=1.1.1.103

Gene names

Name:	tdh
Ordered Locus Names:	Bcen_5157

Organism

Burkholderia cenocepacia (strain AU 1054) [Complete proteome] [HAMAP]

Taxonomic identifier

331271 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex › Burkholderia cenocepacia

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH. HAMAP-Rule MF_00627
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2. HAMAP-Rule MF_00627
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-threonine catabolic process to glycine Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-threonine 3-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	L-threonine 3-dehydrogenase HAMAP-Rule MF_00627		PRO_1000051617
Sites
	Metal binding	38	1	Zinc 1; catalytic By similarity
	Metal binding	63	1	Zinc 1; catalytic By similarity
	Metal binding	93	1	Zinc 2 By similarity
	Metal binding	96	1	Zinc 2 By similarity
	Metal binding	99	1	Zinc 2 By similarity
	Metal binding	107	1	Zinc 2 By similarity
	Metal binding	148	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1BK24 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 71AA3DC7F3ECF29C	FASTA	342	37,358
	10 20 30 40 50 60 MKALAKLERG PGLTLTRVKR PEVGHNDVLI KIRRTAICGT DIHIWKWDDW AQKTIPVPMH 70 80 90 100 110 120 VGHEYVGEIV EMGQEVRGFA IGDRVSGEGH ITCGFCRNCR AGRRHLCRNT VGVGVNREGA 130 140 150 160 170 180 FAEYLAIPAF NAFKIPPEIS DDLASIFDPF GNATHTALSF NLVGEDVLIT GAGPIGIMAV 190 200 210 220 230 240 AIAKHVGARN VVITDINDYR LELARKMGAT RAVNVARESL RDVMADLHMT EGFDVGLEMS 250 260 270 280 290 300 GVPSAFTSLL EAMNHGGKVA LLGIPPAQTA IDWNQVIFKG LEIKGIYGRE MFETWYKMVA 310 320 330 340 MLQSGLDLSP IITHRFAADD YEQGFAAMLS GESGKVILDW TA

References

[1]

"Complete sequence of chromosome 2 of Burkholderia cenocepacia AU 1054." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., LiPuma J.J., Konstantinidis K., Tiedje J.M., Richardson P. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AU 1054.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000379 Genomic DNA. Translation: ABF80031.1.
RefSeq	YP_625004.1. NC_008061.1.
3D structure databases
ProteinModelPortal	Q1BK24.
ModBase	Search...
Protein-protein interaction databases
STRING	331271.Bcen_5157.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABF80031; ABF80031; Bcen_5157.
GeneID	4095105.
KEGG	bcn:Bcen_5157.
PATRIC	19054282. VBIBurCen11237_5345.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1063.
HOGENOM	HOG000294686.
KO	K00060.
OMA	MSIDWNK.
ProtClustDB	PRK05396.
Enzyme and pathway databases
BioCyc	BCEN331271:GHKX-3254-MONOMER.
UniPathway	UPA00046; UER00505.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_00627. Thr_dehydrog.
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR004627. L-Threonine_3-DHase. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. PTHR11695. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
TIGRFAMs	TIGR00692. tdh. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TDH_BURCA

Accession

Primary (citable) accession number: Q1BK24

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 11, 2006
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program