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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Burkholderia cenocepacia (strain AU 1054)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciBCEN331271:GHKX-6150-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:Bcen_6056
OrganismiBurkholderia cenocepacia (strain AU 1054)
Taxonomic identifieri331271 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 858858Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000022330Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ1BHI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini444 – 548105HDUniRule annotationAdd
BLAST
Domaini682 – 76382ACT 1UniRule annotationAdd
BLAST
Domaini790 – 85869ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 324324UridylyltransferaseAdd
BLAST
Regioni325 – 681357Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiSTIGERV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR010043. UTase/UR.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1BHI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAHAAPSPE ALSRRAEFKA AKTEMLERFR HAANVASLMH ALSKLTDDAL
60 70 80 90 100
KRVWDECGLP ATLALVAVGG YGRGELAPYS DVDILVLLPD AHDAALDARI
110 120 130 140 150
ERFIGMAWDL GLEIGSSVRT VAQCIEEASQ DVTVQTSLLE ARRIVGSTAL
160 170 180 190 200
FERFTVRYHE ALDARAFFTA KVLEMRQRHA KFQDTPYSLE PNVKESPGGL
210 220 230 240 250
RDLQTILWIA RAAGFGSSWR ELDTRGLITD REARELRRNE GFLKTLRARL
260 270 280 290 300
HVIAGRRQDM LVFDLQTQAA ESFGYQPTQA KRASEQLMRR YYWAAKAVTQ
310 320 330 340 350
LATILIQNIE AQLFPATSGI TRVLSADRFV EKQGMLEIVD DGVFERHPDA
360 370 380 390 400
ILEAFLLYET TRGVKGLSAR TLRALYNSRE IMNNAWRRDP QNRATFMRIL
410 420 430 440 450
QQPEGITHAF RLMNQTSVLG RYLLNFRRIV GQMQHDLYHV YTVDQHILMV
460 470 480 490 500
LRNIRRFAVA EHAHEYPFCS QLIGNFERPW VLYVAALFHD IAKGRGGDHS
510 520 530 540 550
TLGMADARRF CREHGIAGDD AALIVWLVQH HLTMSQVAQK QDTSDPEVIK
560 570 580 590 600
RFAEVVGNER YLTALYLLTV ADIRGTSPKV WNTWKGKLLE DLYRITLAVL
610 620 630 640 650
GGANPDAHSE LKSRQEQALA LLRLETVPDD AHRALWDQLD VGFFLRHDAA
660 670 680 690 700
DIAWQTRVLY RHVNAETAIV RARPSPIGDA LQVLVYVKDR PDLFAGICAY
710 720 730 740 750
FDRNGLSVLD ARVSTTRHGY ALDNFIVTQT ERDVRYRDIA NLVEQQLATR
760 770 780 790 800
LAETASLPEP SKGRLSRLSR TFPITPRVDL RADERGQYYI LSVSANDRPG
810 820 830 840 850
LLYSIARVLA EHRIGVHAAR INTLGERVED IFLLAGAGLS DNRLQIQLET

ELLRAIAV
Length:858
Mass (Da):96,896
Last modified:July 11, 2006 - v1
Checksum:i407190972C69EE1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000380 Genomic DNA. Translation: ABF80921.1.
RefSeqiWP_006478486.1. NC_008062.1.
YP_625894.1. NC_008062.1.

Genome annotation databases

EnsemblBacteriaiABF80921; ABF80921; Bcen_6056.
KEGGibcn:Bcen_6056.
PATRICi19056150. VBIBurCen11237_6260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000380 Genomic DNA. Translation: ABF80921.1.
RefSeqiWP_006478486.1. NC_008062.1.
YP_625894.1. NC_008062.1.

3D structure databases

ProteinModelPortaliQ1BHI4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF80921; ABF80921; Bcen_6056.
KEGGibcn:Bcen_6056.
PATRICi19056150. VBIBurCen11237_6260.

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiSTIGERV.
OrthoDBiEOG6CCH44.

Enzyme and pathway databases

BioCyciBCEN331271:GHKX-6150-MONOMER.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR010043. UTase/UR.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AU 1054.

Entry informationi

Entry nameiGLND_BURCA
AccessioniPrimary (citable) accession number: Q1BHI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 11, 2006
Last modified: June 24, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.