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Q1BHI4

- GLND_BURCA

UniProt

Q1BHI4 - GLND_BURCA

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Burkholderia cenocepacia (strain AU 1054)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (11 Jul 2006)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciBCEN331271:GHKX-6150-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:Bcen_6056
    OrganismiBurkholderia cenocepacia (strain AU 1054)
    Taxonomic identifieri331271 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
    ProteomesiUP000002400: Chromosome 3

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 858858Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000022330Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi331271.Bcen_6056.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1BHI4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini444 – 548105HDUniRule annotationAdd
    BLAST
    Domaini682 – 76382ACT 1UniRule annotationAdd
    BLAST
    Domaini790 – 85869ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 324324UridylyltransferaseAdd
    BLAST
    Regioni325 – 681357Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261778.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1BHI4-1 [UniParc]FASTAAdd to Basket

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    MSAHAAPSPE ALSRRAEFKA AKTEMLERFR HAANVASLMH ALSKLTDDAL    50
    KRVWDECGLP ATLALVAVGG YGRGELAPYS DVDILVLLPD AHDAALDARI 100
    ERFIGMAWDL GLEIGSSVRT VAQCIEEASQ DVTVQTSLLE ARRIVGSTAL 150
    FERFTVRYHE ALDARAFFTA KVLEMRQRHA KFQDTPYSLE PNVKESPGGL 200
    RDLQTILWIA RAAGFGSSWR ELDTRGLITD REARELRRNE GFLKTLRARL 250
    HVIAGRRQDM LVFDLQTQAA ESFGYQPTQA KRASEQLMRR YYWAAKAVTQ 300
    LATILIQNIE AQLFPATSGI TRVLSADRFV EKQGMLEIVD DGVFERHPDA 350
    ILEAFLLYET TRGVKGLSAR TLRALYNSRE IMNNAWRRDP QNRATFMRIL 400
    QQPEGITHAF RLMNQTSVLG RYLLNFRRIV GQMQHDLYHV YTVDQHILMV 450
    LRNIRRFAVA EHAHEYPFCS QLIGNFERPW VLYVAALFHD IAKGRGGDHS 500
    TLGMADARRF CREHGIAGDD AALIVWLVQH HLTMSQVAQK QDTSDPEVIK 550
    RFAEVVGNER YLTALYLLTV ADIRGTSPKV WNTWKGKLLE DLYRITLAVL 600
    GGANPDAHSE LKSRQEQALA LLRLETVPDD AHRALWDQLD VGFFLRHDAA 650
    DIAWQTRVLY RHVNAETAIV RARPSPIGDA LQVLVYVKDR PDLFAGICAY 700
    FDRNGLSVLD ARVSTTRHGY ALDNFIVTQT ERDVRYRDIA NLVEQQLATR 750
    LAETASLPEP SKGRLSRLSR TFPITPRVDL RADERGQYYI LSVSANDRPG 800
    LLYSIARVLA EHRIGVHAAR INTLGERVED IFLLAGAGLS DNRLQIQLET 850
    ELLRAIAV 858
    Length:858
    Mass (Da):96,896
    Last modified:July 11, 2006 - v1
    Checksum:i407190972C69EE1E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000380 Genomic DNA. Translation: ABF80921.1.
    RefSeqiYP_625894.1. NC_008062.1.

    Genome annotation databases

    EnsemblBacteriaiABF80921; ABF80921; Bcen_6056.
    GeneIDi4097107.
    KEGGibcn:Bcen_6056.
    PATRICi19056150. VBIBurCen11237_6260.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000380 Genomic DNA. Translation: ABF80921.1 .
    RefSeqi YP_625894.1. NC_008062.1.

    3D structure databases

    ProteinModelPortali Q1BHI4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 331271.Bcen_6056.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABF80921 ; ABF80921 ; Bcen_6056 .
    GeneIDi 4097107.
    KEGGi bcn:Bcen_6056.
    PATRICi 19056150. VBIBurCen11237_6260.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261778.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci BCEN331271:GHKX-6150-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AU 1054.

    Entry informationi

    Entry nameiGLND_BURCA
    AccessioniPrimary (citable) accession number: Q1BHI4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: July 11, 2006
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3