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Q1B9R1 (RUVC_MYCSS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:Mmcs_2265
OrganismMycobacterium sp. (strain MCS) [Complete proteome] [HAMAP]
Taxonomic identifier164756 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length185 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Sequence caution

The sequence ABG08373.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 185185Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000332430

Sites

Metal binding71Magnesium By similarity
Metal binding681Magnesium By similarity
Metal binding1411Magnesium By similarity
Metal binding1441Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1B9R1 [UniParc].

Last modified April 29, 2008. Version 2.
Checksum: 518663865694EE91

FASTA18519,860
        10         20         30         40         50         60 
MRVMGVDPGL TRCGLSVIES GQGRKVIALD VDVVRTPADE QLHRRLLIIS DTVEHWMDTH 

        70         80         90        100        110        120 
RPDVIAIERV FANHNANTAM GTAQAGGVIA LAAAKRDIDV HFHTPSEVKA AVTGNGRADK 

       130        140        150        160        170        180 
AQVTEMVTRI LALQAKPTPA DAADALALAI CHCWRAPMIA RMAAAEAMAA EARRKYQAKL 


KAARA 

« Hide

References

[1]"Complete sequence of chromosome of Mycobacterium sp. MCS."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. expand/collapse author list , Miller C.D., Hughes J.E., Anderson A.J., Sims R.C., Richardson P.
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MCS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000384 Genomic DNA. Translation: ABG08373.1. Different initiation.
RefSeqYP_639429.1. NC_008146.1.

3D structure databases

ProteinModelPortalQ1B9R1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING164756.Mmcs_2265.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG08373; ABG08373; Mmcs_2265.
GeneID4111098.
KEGGmmc:Mmcs_2265.
PATRIC18114744. VBIMycSp106721_2321.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012182.
KOK01159.
OrthoDBEOG6RG044.
ProtClustDBPRK00039.

Enzyme and pathway databases

BioCycMSP164756:GHQ8-2283-MONOMER.

Family and domain databases

HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_MYCSS
AccessionPrimary (citable) accession number: Q1B9R1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: February 19, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families