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Protein

S-adenosylmethionine synthase

Gene

metK

Organism
Mycobacterium sp. (strain MCS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.UniRule annotation

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 divalent ions per subunit.UniRule annotation
  • K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (metK)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16ATPUniRule annotation1
Metal bindingi18MagnesiumUniRule annotation1
Metal bindingi44PotassiumUniRule annotation1
Binding sitei57MethionineUniRule annotation1
Binding sitei103MethionineUniRule annotation1
Binding sitei258ATP; shared with neighboring subunitUniRule annotation1
Binding sitei258Methionine; shared with neighboring subunitUniRule annotation1
Binding sitei281ATP; via amide nitrogen; shared with neighboring subunitUniRule annotation1
Binding sitei285ATP; shared with neighboring subunitUniRule annotation1
Binding sitei289MethionineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 180ATPUniRule annotation3
Nucleotide bindingi249 – 250ATPUniRule annotation2
Nucleotide bindingi264 – 265ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processOne-carbon metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
Short name:
AdoMet synthaseUniRule annotation
Alternative name(s):
MATUniRule annotation
Methionine adenosyltransferaseUniRule annotation
Gene namesi
Name:metKUniRule annotation
Ordered Locus Names:Mmcs_2373
OrganismiMycobacterium sp. (strain MCS)
Taxonomic identifieri164756 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003029441 – 402S-adenosylmethionine synthaseAdd BLAST402

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ1B9F3.
SMRiQ1B9F3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 113Flexible loopUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the AdoMet synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000245710.
KOiK00789.
OMAiDNFLAFD.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1. 1 hit.
InterProiView protein in InterPro
IPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiView protein in Pfam
PF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiView protein in PROSITE
PS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1B9F3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKGRLFTSE SVTEGHPDKI CDAISDSVLD SLLAQDPRSR VAVETLVTTG
60 70 80 90 100
QVHVVGEVTT SAKEAFADIT NTVRHRILEV GYDSSDKGFD GTTCGVNIGI
110 120 130 140 150
GAQSPDIAQG VDTAHETRVE GAGDPLDLQG AGDQGLMFGY AIKDTPELMP
160 170 180 190 200
LPIALAHRLA RRLTEVRKNG VLDYLRPDGK TQVTVQYDGV TPVRLDTVVL
210 220 230 240 250
STQHAEGIDL EGTLTPDIRE KVVNTVLSDL NHDSMDTSDF RLLVNPTGKF
260 270 280 290 300
VLGGPMGDAG LTGRKIIVDT YGGWARHGGG AFSGKDPSKV DRSAAYAMRW
310 320 330 340 350
VAKNVVAAGL ADRVEVQVAY AIGKAAPVGL FVETFGTETV DPAKIEKAIT
360 370 380 390 400
EVFDLRPGAI VRDLDLLRPI YAPTAAYGHF GRTDIDLPWE RTDKADALKT

AV
Length:402
Mass (Da):42,979
Last modified:July 11, 2006 - v1
Checksum:i918BC6F232095500
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000384 Genomic DNA. Translation: ABG08481.1.
RefSeqiWP_011559770.1. NC_008146.1.

Genome annotation databases

EnsemblBacteriaiABG08481; ABG08481; Mmcs_2373.
KEGGimmc:Mmcs_2373.
PATRICi18114964. VBIMycSp106721_2431.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000384 Genomic DNA. Translation: ABG08481.1.
RefSeqiWP_011559770.1. NC_008146.1.

3D structure databases

ProteinModelPortaliQ1B9F3.
SMRiQ1B9F3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG08481; ABG08481; Mmcs_2373.
KEGGimmc:Mmcs_2373.
PATRICi18114964. VBIMycSp106721_2431.

Phylogenomic databases

HOGENOMiHOG000245710.
KOiK00789.
OMAiDNFLAFD.

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1. 1 hit.
InterProiView protein in InterPro
IPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiView protein in Pfam
PF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiView protein in PROSITE
PS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMETK_MYCSS
AccessioniPrimary (citable) accession number: Q1B9F3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 11, 2006
Last modified: March 15, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.