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Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium sp. (strain MCS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei277Acetyl-CoAUniRule annotation1
Binding sitei314Acetyl-CoAUniRule annotation1
Active sitei342Proton acceptorUniRule annotation1
Binding sitei342GlyoxylateUniRule annotation1
Metal bindingi437MagnesiumUniRule annotation1
Binding sitei437GlyoxylateUniRule annotation1
Metal bindingi465MagnesiumUniRule annotation1
Binding sitei546Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei636Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Mmcs_2817
OrganismiMycobacterium sp. (strain MCS)
Taxonomic identifieri164756 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000569091 – 731Malate synthase GAdd BLAST731

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei622Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ1B860.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni462 – 465Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1B860-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRVTVGNL RVARALYDFI TDEALAGTDL DPDSFWSGVD KVVADLTPRN
60 70 80 90 100
QELLARRDDL QAQIDKWHRQ RAIGPHDADE YKQFLTEIGY LEPDPGDFTI
110 120 130 140 150
TTAGVDDEIT TTAGPQLVVP VLNARFALNA ANARWGSLYD ALYGTDVISE
160 170 180 190 200
EDGAEKGTSY NRVRGDKVIA YAREFLDGAA PLASGSYADA TGFRIEDGQV
210 220 230 240 250
QVELGDDQWV GLADPDQFVG YTGELGSPQW SILLRNNGLH IEILIDPDSP
260 270 280 290 300
VGSTDKAGVK DVVLESAVTT IMDFEDSVAA VDAEDKVLGY RNWLGLNRGD
310 320 330 340 350
LSEEVSKGDK TFTRVLNPDR TYTTPDGSGE LTLPGRSLLF VRNVGHLMTN
360 370 380 390 400
DAITDAEGNE VFEGIQDALF TGLIAMHGLK ESDANGPLRN SRTGSVYIVK
410 420 430 440 450
PKMHGPAEVA YTVDLFSRVE DVLGLPQNTL KVGIMDEERR TTLNLKACIK
460 470 480 490 500
AAADRVVFIN TGFLDRTGDE IHTSMEAGPM IRKGAMKSQP WIKAYEDQNV
510 520 530 540 550
DVGLATGFSG RAQIGKGMWA MTDLMADMVE QKIGQPKAGA TTAWVPSPTA
560 570 580 590 600
ATLHAMHYHQ VDVYAVHKEL EGKQRASLDD LLTIPLAKEL AWAPEEIREE
610 620 630 640 650
VDNNCQSILG YVVRWIDAGV GCSKVPDIHD IALMEDRATL RISSQLLANW
660 670 680 690 700
LRHGVITEED VKTSLRRMAA VVDEQNAKDP DFKPMATDPD SSIAFQAAQE
710 720 730
LILAGGTQPS GYTEPILHRR RREYKASVAG A
Length:731
Mass (Da):79,921
Last modified:July 11, 2006 - v1
Checksum:i05AB383646FED936
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000384 Genomic DNA. Translation: ABG08924.1.
RefSeqiWP_011560210.1. NC_008146.1.

Genome annotation databases

EnsemblBacteriaiABG08924; ABG08924; Mmcs_2817.
KEGGimmc:Mmcs_2817.
PATRICi18115889. VBIMycSp106721_2893.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000384 Genomic DNA. Translation: ABG08924.1.
RefSeqiWP_011560210.1. NC_008146.1.

3D structure databases

ProteinModelPortaliQ1B860.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG08924; ABG08924; Mmcs_2817.
KEGGimmc:Mmcs_2817.
PATRICi18115889. VBIMycSp106721_2893.

Phylogenomic databases

HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMASZ_MYCSS
AccessioniPrimary (citable) accession number: Q1B860
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 11, 2006
Last modified: November 30, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.