SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q1B7F4

- BIOB_MYCSS

UniProt

Q1B7F4 - BIOB_MYCSS

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Biotin synthase

Gene
bioB, Mmcs_3073
Organism
Mycobacterium sp. (strain MCS)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi110 – 1101Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi143 – 1431Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi202 – 2021Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi272 – 2721Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMSP164756:GHQ8-3099-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Mmcs_3073
OrganismiMycobacterium sp. (strain MCS)
Taxonomic identifieri164756 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000001972: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Biotin synthaseUniRule annotationPRO_0000381485Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi164756.Mmcs_3073.

Structurei

3D structure databases

ProteinModelPortaliQ1B7F4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiTCENTLR.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1B7F4-1 [UniParc]FASTAAdd to Basket

« Hide

MSDILAVARE QVLERGVGLN QDQTLQVLQL PDDRLDDLLA LAHEVRMAWC    50
GPDVEVEGII SLKTGGCPED CHFCSQSGLF ASPVRSAWLD VPSLVEAAKQ 100
TAKTGATEFC IVAAVRGPDE RLLAQVAAGI EAIRNEVDIQ IACSLGMLTA 150
EQVERLSEMG VHRYNHNLET ARSFFTNVVT THTWEERWDT LRMVREAGME 200
VCCGGILGMG ETLEQRAEFA ANLAELDPHE VPLNFLNPRP GTPFGDLEVL 250
PAAEALKAVA AFRLALPRTM LRFAGGREIT LGDLGAKKGI LGGINAVIVG 300
NYLTTLGRPA EADLELLDDL QMPIKALNAS L 331
Length:331
Mass (Da):35,821
Last modified:July 11, 2006 - v1
Checksum:i6950BAF5C60D6F69
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000384 Genomic DNA. Translation: ABG09180.1.
RefSeqiYP_640236.1. NC_008146.1.

Genome annotation databases

EnsemblBacteriaiABG09180; ABG09180; Mmcs_3073.
GeneIDi4111905.
KEGGimmc:Mmcs_3073.
PATRICi18116417. VBIMycSp106721_3151.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000384 Genomic DNA. Translation: ABG09180.1 .
RefSeqi YP_640236.1. NC_008146.1.

3D structure databases

ProteinModelPortali Q1B7F4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 164756.Mmcs_3073.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG09180 ; ABG09180 ; Mmcs_3073 .
GeneIDi 4111905.
KEGGi mmc:Mmcs_3073.
PATRICi 18116417. VBIMycSp106721_3151.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi TCENTLR.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MSP164756:GHQ8-3099-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MCS.

Entry informationi

Entry nameiBIOB_MYCSS
AccessioniPrimary (citable) accession number: Q1B7F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 11, 2006
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi