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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Mycobacterium sp. (strain MCS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431ZincUniRule annotation
Binding sitei58 – 581Cysteinyl adenylateUniRule annotation
Binding sitei227 – 2271Cysteinyl adenylateUniRule annotation
Metal bindingi231 – 2311ZincUniRule annotation
Metal bindingi256 – 2561ZincUniRule annotation
Binding sitei283 – 2831Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMSP164756:GHQ8-3179-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:Mmcs_3153
OrganismiMycobacterium sp. (strain MCS)
Taxonomic identifieri164756 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligasePRO_0000400465Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ1B774.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 464Cysteinyl adenylate bindingUniRule annotation
Regioni81 – 833Cysteinyl adenylate bindingUniRule annotation
Regioni249 – 2513Cysteinyl adenylate bindingUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi45 – 5511"HIGH" regionUniRule annotationAdd
BLAST
Motifi187 – 1926"ERGGDP" regionUniRule annotation
Motifi289 – 2935"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000245251.
KOiK15526.
OMAiILAHHYR.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1B774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESWSAPEVP ALPGRGPQLR LYDSADRQVR PVSAGDTATM YVCGITPYDA
60 70 80 90 100
THLGHAATYL AFDLVHRLWL DAGHRVHYVQ NITDVDDPLF ERAARDGIDW
110 120 130 140 150
RDLGAREIQL FREDMAALRV LPPHDYVAAT DAIAEVIELV EKMLASGAAY
160 170 180 190 200
VVDDPEFPDV YYRADATVQF GYESNYDHET MLTLFAERGG DPDRAGKADE
210 220 230 240 250
LDALLWRAER PGEPSWPSPF GPGRPGWHVE CAAIALSRIG TGLDIQGGGS
260 270 280 290 300
DLIFPHHEFS AAHAESVTGE RRFARHYVHA GMIGWDGHKM SKSRGNLVLV
310 320 330 340 350
SRLRAEGVDP SAIRLGLLAG HYREDRFWSD DVLSDAQTRL QRWRRATSLP
360 370 380 390 400
TGPDATDVLA RVRTYLADDL DTPKALIALD AWCTEALDGG GSDVTAPKTV
410
ATAVDALLGV ALPVE
Length:415
Mass (Da):45,517
Last modified:July 11, 2006 - v1
Checksum:iC99447E17632E85F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000384 Genomic DNA. Translation: ABG09260.1.
RefSeqiWP_011560546.1. NC_008146.1.

Genome annotation databases

EnsemblBacteriaiABG09260; ABG09260; Mmcs_3153.
KEGGimmc:Mmcs_3153.
PATRICi18116583. VBIMycSp106721_3234.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000384 Genomic DNA. Translation: ABG09260.1.
RefSeqiWP_011560546.1. NC_008146.1.

3D structure databases

ProteinModelPortaliQ1B774.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG09260; ABG09260; Mmcs_3153.
KEGGimmc:Mmcs_3153.
PATRICi18116583. VBIMycSp106721_3234.

Phylogenomic databases

HOGENOMiHOG000245251.
KOiK15526.
OMAiILAHHYR.

Enzyme and pathway databases

BioCyciMSP164756:GHQ8-3179-MONOMER.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHC_MYCSS
AccessioniPrimary (citable) accession number: Q1B774
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: July 11, 2006
Last modified: September 7, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.