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Q1B6B3 (MBTG_MYCSS) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
L-lysine 6-monooxygenase mbtG
EC=1.14.13.59
Alternative name(s):
Lysine 6-N-hydroxylase
Lysine N(6)-hydroxylase
Lysine-N-oxygenase
Mycobactin synthase protein G
Gene names
Name:mbtG
Ordered Locus Names:Mmcs_3464
OrganismMycobacterium sp. (strain MCS) [Complete proteome] [HAMAP]
Taxonomic identifier164756 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length430 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Flavoprotein monooxygenase required for N-hydroxylation of the two acylated lysine residues during mycobactin assembly, thus producing the hydroxamate groups necessary for iron sequestration. Is also able, but less efficiently, to hydroxylate L-lysine (non acylated) in vitro By similarity.

Catalytic activity

L-lysine + NADPH + O2 = N(6)-hydroxy-L-lysine + NADP+ + H2O.

Cofactor

FAD By similarity.

Pathway

Siderophore biosynthesis; mycobactin biosynthesis.

Sequence similarities

Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family.

Ontologies

Keywords
   DomainSignal
   LigandFAD
Flavoprotein
NADP
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functionL-lysine 6-monooxygenase (NADPH) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 430409L-lysine 6-monooxygenase mbtG
PRO_0000261311

Sequences

Sequence LengthMass (Da)Tools
Q1B6B3 [UniParc].

Last modified July 11, 2006. Version 1.
Checksum: 2F943638F21DD3A2

FASTA43046,622
        10         20         30         40         50         60 
MTATLAVIGA GPKAVAVAAK AAELRNMGVD APDVVVVERA GVGANWTAAG GWTDGQHRLG 

        70         80         90        100        110        120 
TSPEKDIGFP YRSSLVPRRN AELDDRMTRH SWQAYLVATS QFAEWIDRGR PAPNHHRWAA 

       130        140        150        160        170        180 
YLRWVADAIG MNVVHGEVER ISIGGRGWEL HTPESTVAAD AVMITGPGQA ERTLLPGHPR 

       190        200        210        220        230        240 
VMSIADFWRR TAGHELIVAE RVAMIGGGET AASMLNELFR HRVSTITVIS PQVTLFTRGE 

       250        260        270        280        290        300 
GFFENTLFSD PTGWTSLTLA ERRDAMFRTD RGVFSARVQE ALLADDRIRH LRGRVAHAVP 

       310        320        330        340        350        360 
RDGRIRLTLH TDRAGERVET VHGFDLVIDG QGADALWFLP LLGQDARDLL ELGLGGPLTG 

       370        380        390        400        410        420 
ELLQESIGHD LAVGGVTPKL FLPGLAGLNQ GPGFPNLSCL GLMSDRILGA DLGANAAMTN 

       430 
RRSIEHQPIR

« Hide

References

[1]"Complete sequence of chromosome of Mycobacterium sp. MCS."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. expand/collapse author list , Miller C.D., Hughes J.E., Anderson A.J., Sims R.C., Richardson P.
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MCS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000384 Genomic DNA. Translation: ABG09571.1.
RefSeqYP_640627.1. NC_008146.1.

3D structure databases

ProteinModelPortalQ1B6B3.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ1B6B3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000059553; EBMYCP00000057720; EBMYCG00000059548.
GeneID4112296.
GenomeReviewsGene locus Mmcs_3464 in contig CP000384_GR.
KEGGmmc:Mmcs_3464.
PATRIC18117225. VBIMycSp106721_3552.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG13470.
GeneTreeEBGT00050000017267.
HOGENOMHBG432039.
OMAIAQFWHR.
ProtClustDBCLSK791811.

Enzyme and pathway databases

BioCycMSP164756:MMCS_3464-MONOMER.

Family and domain databases

InterProIPR013027. FAD_pyr_nucl-diS_OxRdtase.
[Graphical view]
KOK04793.
PRINTSPR00368. FADPNR.
ProtoNetSearch...

Entry information

Entry nameMBTG_MYCSS
AccessionPrimary (citable) accession number: Q1B6B3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 11, 2006
Last modified: January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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