Reviewed, UniProtKB/Swiss-Prot Q1B6B3 (MBTG_MYCSS)

Names and origin

Protein names

Recommended name:
    L-lysine 6-monooxygenase mbtG
    EC=1.14.13.59
Alternative name(s):
    Lysine 6-N-hydroxylase
    Lysine N(6)-hydroxylase
    Lysine-N-oxygenase
    Mycobactin synthetase protein G

Gene names

Name:	mbtG
Ordered Locus Names:	Mmcs_3464

Organism

Mycobacterium sp. (strain MCS) [Complete proteome] [HAMAP]

Taxonomic identifier

164756 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Flavoprotein monooxygenase required for N-hydroxylation of the two acylated lysine residues during mycobactin assembly, thus producing the hydroxamate groups necessary for iron sequestration. Is also able, but less efficiently, to hydroxylate L-lysine (non acylated) in vitro By similarity.
Catalytic activity	L-lysine + NADPH + O2 = N(6)-hydroxy-L-lysine + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Siderophore biosynthesis; mycobactin biosynthesis.
Sequence similarities	Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family.

Ontologies

Keywords
Biological process	Ion transport Iron transport Transport
Domain	Signal
Ligand	FAD Flavoprotein Iron NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	iron ion transport Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-lysine 6-monooxygenase (NADPH) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 430	409	L-lysine 6-monooxygenase mbtG		PRO_0000261311

Sequences

	Sequence		Length	Mass (Da)
	Q1B6B3-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 2F943638F21DD3A2	FASTA	430	46,622
	10 20 30 40 50 60 MTATLAVIGA GPKAVAVAAK AAELRNMGVD APDVVVVERA GVGANWTAAG GWTDGQHRLG 70 80 90 100 110 120 TSPEKDIGFP YRSSLVPRRN AELDDRMTRH SWQAYLVATS QFAEWIDRGR PAPNHHRWAA 130 140 150 160 170 180 YLRWVADAIG MNVVHGEVER ISIGGRGWEL HTPESTVAAD AVMITGPGQA ERTLLPGHPR 190 200 210 220 230 240 VMSIADFWRR TAGHELIVAE RVAMIGGGET AASMLNELFR HRVSTITVIS PQVTLFTRGE 250 260 270 280 290 300 GFFENTLFSD PTGWTSLTLA ERRDAMFRTD RGVFSARVQE ALLADDRIRH LRGRVAHAVP 310 320 330 340 350 360 RDGRIRLTLH TDRAGERVET VHGFDLVIDG QGADALWFLP LLGQDARDLL ELGLGGPLTG 370 380 390 400 410 420 ELLQESIGHD LAVGGVTPKL FLPGLAGLNQ GPGFPNLSCL GLMSDRILGA DLGANAAMTN 430 RRSIEHQPIR

References

[1]

"Complete sequence of chromosome of Mycobacterium sp. MCS." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. , Miller C.D., Hughes J.E., Anderson A.J., Sims R.C., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000384 Genomic DNA. Translation: ABG09571.1.
RefSeq	YP_640627.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4112296.
GenomeReviews	Gene locus Mmcs_3464 in contig CP000384_GR.
KEGG	mmc:Mmcs_3464.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1B6B3.
OMA	Q1B6B3. SIAQFWH.
Enzyme and pathway databases
BioCyc	MSP164756:MMCS_3464-MON.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

MBTG_MYCSS

Accession

Primary (citable) accession number: Q1B6B3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	July 11, 2006
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)