Q1B585 (RNPH_MYCSS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	Mmcs_3844

Organism

Mycobacterium sp. (strain MCS) [Complete proteome] [HAMAP]

Taxonomic identifier

164756 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Ribonuclease PH HAMAP MF_00564		PRO_1000024833

Sequences

	Sequence		Length	Mass (Da)
	Q1B585 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 109A40CE4BB9A784	FASTA	260	27,647
	10 20 30 40 50 60 MSRREDGRLD DELRPVRITR GFTSHPAGSV LVEFGETRVM CTASVTEGVP RWRKGTGQGW 70 80 90 100 110 120 LTAEYAMLPA ATHDRSDRES VKGRVGGRTQ EISRLIGRSL RACIDLNALG ENTIAIDCDV 130 140 150 160 170 180 LQADGGTRTA AITGAYVALA DAVTYLAAAE KLSDPRPLSC AIAAVSVGVV DGRVRVDLPY 190 200 210 220 230 240 SEDSRAEVDM NVVATDTGTL VEIQGTGEGA TFPRSTLDKL LDLALASCDQ LFVVQREALD 250 260 APYPGALPEP TSPPKKAFGS

References

[1]

"Complete sequence of chromosome of Mycobacterium sp. MCS." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. , Miller C.D., Hughes J.E., Anderson A.J., Sims R.C., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MCS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000384 Genomic DNA. Translation: ABG09949.1.
RefSeq	YP_641005.1. NC_008146.1.
3D structure databases
ProteinModelPortal	Q1B585.
SMR	Q1B585. Positions 3-248.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1B585.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000060041; EBMYCP00000058208; EBMYCG00000060036.
GeneID	4112674.
GenomeReviews	Gene locus Mmcs_3844 in contig CP000384_GR.
KEGG	mmc:Mmcs_3844.
PATRIC	18118013. VBIMycSp106721_3940.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
GeneTree	EBGT00050000017525.
HOGENOM	HBG737187.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	MSP164756:MMCS_3844-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_MYCSS

Accession

Primary (citable) accession number: Q1B585

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 11, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program