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Q1AZ86 (GLGB_RUBXD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Rxyl_0316
OrganismRubrobacter xylanophilus (strain DSM 9941 / NBRC 16129) [Complete proteome] [HAMAP]
Taxonomic identifier266117 [NCBI]
Taxonomic lineageBacteriaActinobacteriaRubrobacteridaeRubrobacteralesRubrobacterineaeRubrobacteraceaeRubrobacter

Protein attributes

Sequence length722 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7227221,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260694

Sites

Active site4011Nucleophile By similarity
Active site4541Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1AZ86 [UniParc].

Last modified July 11, 2006. Version 1.
Checksum: 8C113B22C783CEA5

FASTA72281,740
        10         20         30         40         50         60 
MRREEAIRAV VAGDHPDPFS FLGPHEEAGR RVVRTFLPGA ARVRVLSAAG RPLGELERVH 

        70         80         90        100        110        120 
PEGFFSGPLS GNGRYRLRVL WEGGGEDELE DPYRFPPVLS DYDLYLFGEG NNHRIYRHLG 

       130        140        150        160        170        180 
AHPAEMDGVC GTAFAVWAPN ARRVSVVGDF NLWDGRRHPM RNRNGVWEIF LPGVGPGALY 

       190        200        210        220        230        240 
KYEIKDAGGS LLPLKADPYG FFAEQYPGTA SIVWDLSRHR WEDGEWMERR GSRNAPDAPM 

       250        260        270        280        290        300 
AIYEVHLGSW RRRPDGSHLT YRELAEELVP YVAGLGYTHV ELLPPMEHPF GGSWGYQPVG 

       310        320        330        340        350        360 
LFAPTSRFGP PEDFKHLVDA FHRAGVGVIA DWVPAHFPED AHGLARFDGT HLYEHADPRK 

       370        380        390        400        410        420 
GRHPDWGTLI YNYGRNEVRN FLISNALFWL DEYHIDGLRV DAVASMLYLD YSRKEGEWVP 

       430        440        450        460        470        480 
NEHGGNENLE AIAFLRRMNE VVYGEAPGAF TVAEESTAWP MVSRPTYMGG LGFGYKWNMG 

       490        500        510        520        530        540 
WMHDTLQYMK EDPVHRRYHH DRITFGLLYA FNENFILPLS HDEVVHGKGS LLGRMPGDRW 

       550        560        570        580        590        600 
QRFANLRAYY GFMYGHPGKQ LLFMGGEFAQ EREWDSGSQL DWHLLEGGEN RGVRDLVADL 

       610        620        630        640        650        660 
NALYRATPAL HQVDFEPEGF EWVEGGDAEQ SVVSFLRRAR NPEDFVLVVS NFTPVVRHGY 

       670        680        690        700        710        720 
RVGVPASGPY AEVLNTDDPR YGGSGVANGE LEAEEVPWHG RPFSLRLTLP PLATVFLRPR 


PV 

« Hide

References

[1]"Complete sequence of Rubrobacter xylanophilus DSM 9941."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., da Costa M.S., Rainey F.A., Empadinhas N., Jolivet E., Battista J.R., Richardson P.
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 9941 / NBRC 16129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000386 Genomic DNA. Translation: ABG03292.1.
RefSeqYP_643104.1. NC_008148.1.

3D structure databases

ProteinModelPortalQ1AZ86.
SMRQ1AZ86. Positions 112-721.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266117.Rxyl_0316.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABG03292; ABG03292; Rxyl_0316.
GeneID4114777.
KEGGrxy:Rxyl_0316.
PATRIC23367428. VBIRubXyl52678_0328.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycRXYL266117:GH8O-320-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_RUBXD
AccessionPrimary (citable) accession number: Q1AZ86
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 11, 2006
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries