Q1AXL8 (ISPE_RUBXD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Rxyl_0893

Organism

Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129) [Complete proteome] [HAMAP]

Taxonomic identifier

266117 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Rubrobacteridae › Rubrobacterales › Rubrobacterineae › Rubrobacteraceae › Rubrobacter

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335751
Regions
	Nucleotide binding	96 – 106	11	ATP Potential
Sites
	Active site	12	1	By similarity
	Active site	138	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1AXL8 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 27B4C0AD79EB1428	FASTA	292	29,498
	10 20 30 40 50 60 MRGSLRVRAF AKVNYALEVL GRRADGYHEI RTVLQSVSLW DELEISGPEG RFGLVVEPEG 70 80 90 100 110 120 AGVGPERENT VYRAWRLLAG EAGAPPVRVT LRKGIPAGSG LGGGSADAAA LLWGANELLG 130 140 150 160 170 180 LGLGEADLAA LALRVGADVP FCLVGGTALG EGVGERLFPL PAPPEHRLVV AMPPRGASTA 190 200 210 220 230 240 EVYGAYGGLG GAAPGGWSGR VVRALRAGDL GALGAAVGND LTPVTAGLAP EVEKLLAGLR 250 260 270 280 290 EAGALGACMS GTGSAVYGLF REEAAAHEAA RRLGGAAFVR VCRPVGRGLA GG

References

[1]

"Complete sequence of Rubrobacter xylanophilus DSM 9941." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., da Costa M.S., Rainey F.A., Empadinhas N., Jolivet E., Battista J.R., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 9941 / NBRC 16129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000386 Genomic DNA. Translation: ABG03860.1.
RefSeq	YP_643672.1. NC_008148.1.
3D structure databases
ProteinModelPortal	Q1AXL8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1AXL8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4116988.
GenomeReviews	Gene locus Rxyl_0893 in contig CP000386_GR.
KEGG	rxy:Rxyl_0893.
NMPDR	fig|266117.6.peg.816.
PATRIC	23368602. VBIRubXyl52678_0914.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	KINLHLG.
PhylomeDB	Q1AXL8.
Enzyme and pathway databases
BioCyc	RXYL266117:RXYL_0893-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_RUBXD

Accession

Primary (citable) accession number: Q1AXL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 11, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program