Q1AX25 (SYP_RUBXD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Rxyl_1087

Organism

Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129) [Complete proteome] [HAMAP]

Taxonomic identifier

266117 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Rubrobacteridae › Rubrobacterales › Rubrobacterineae › Rubrobacteraceae › Rubrobacter › Rubrobacter xylanophilus

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP-Rule MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01571
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP-Rule MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Proline--tRNA ligase HAMAP-Rule MF_01571		PRO_0000288414

Sequences

	Sequence		Length	Mass (Da)
	Q1AX25 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: FF54D8996514A556	FASTA	476	54,544
	10 20 30 40 50 60 MTEAVERLTK KSEDSSRWYL ELVRMAKLAD YGPVRGTFAI RPYGFAIWER IQADLDARFK 70 80 90 100 110 120 ATGHVNAYFP LLIPESYLTK EAEHVEGFAP ECAWVTVGGD DELEERLAIR PTSESIICDF 130 140 150 160 170 180 YRKWIHSYRD LPVLINQWCN VLRWEMVTRP FLRTAEFLWQ EGHTVHATAE EAREEALRML 190 200 210 220 230 240 NVYRDCFYEV LAIPVLTGMK SPSERFAGAV ETFTCEGLMG DGRALQAATS HDLGQNFARA 250 260 270 280 290 300 FDITFLDENQ ERVHPYQTSW GFSTRTIGAL ILVHGDDRGL RLPPKLAPTQ AVVVPIWRGK 310 320 330 340 350 360 NKGEVRREAE ALHRELAEAG LRAEADLDEE HSPGWKFNEH ELRGVPVRVE LGPKDIEKGQ 370 380 390 400 410 420 AVLVRRDTGE KEFVGRGAAA RRLVELMDEI QQNMLRQAEA FRDENTRRAE TYEEFKEIIE 430 440 450 460 470 EKRGFVVAPW DGTEETEQRI KEETKATIRL LPFEREEGKD LVSGRPGKTA VFARAY

References

[1]

"Complete sequence of Rubrobacter xylanophilus DSM 9941." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., da Costa M.S., Rainey F.A., Empadinhas N., Jolivet E., Battista J.R., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 9941 / NBRC 16129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000386 Genomic DNA. Translation: ABG04053.1.
RefSeq	YP_643865.1. NC_008148.1.
3D structure databases
ProteinModelPortal	Q1AX25.
SMR	Q1AX25. Positions 6-476.
ModBase	Search...
Protein-protein interaction databases
STRING	266117.Rxyl_1087.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABG04053; ABG04053; Rxyl_1087.
GeneID	4116675.
KEGG	rxy:Rxyl_1087.
PATRIC	23369020. VBIRubXyl52678_1115.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HOG000167538.
KO	K01881.
OMA	EGFAPEC.
Enzyme and pathway databases
BioCyc	RXYL266117:GH8O-1151-MONOMER.
Family and domain databases
Gene3D	3.30.110.30. 1 hit. 3.40.50.800. 1 hit.
HAMAP	MF_01571. Pro_tRNA_synth_type3.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-ligase_IIa. IPR004499. Pro-tRNA-ligase_IIa_arc-type. IPR016061. Pro-tRNA_ligase_II_C. IPR017449. Pro-tRNA_synth_II. [Graphical view]
PANTHER	PTHR11451:SF6. PTHR11451:SF6. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. proS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_RUBXD

Accession

Primary (citable) accession number: Q1AX25

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 11, 2006
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program