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Protein

Proline--tRNA ligase

Gene

proS

Organism
Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRXYL266117:GH8O-1104-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:Rxyl_1087
OrganismiRubrobacter xylanophilus (strain DSM 9941 / NBRC 16129)
Taxonomic identifieri266117 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaRubrobacteriaRubrobacteralesRubrobacterineaeRubrobacteraceaeRubrobacter
ProteomesiUP000006637 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476Proline--tRNA ligasePRO_0000288414Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi266117.Rxyl_1087.

Structurei

3D structure databases

ProteinModelPortaliQ1AX25.
SMRiQ1AX25. Positions 6-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0442.
HOGENOMiHOG000167538.
KOiK01881.
OMAiEGFAPEC.
OrthoDBiEOG6G7R2R.

Family and domain databases

Gene3Di3.30.110.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_01571. Pro_tRNA_synth_type3.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004499. Pro-tRNA-ligase_IIa_arc-type.
IPR016061. Pro-tRNA_ligase_II_C.
IPR017449. Pro-tRNA_synth_II.
[Graphical view]
PANTHERiPTHR11451:SF6. PTHR11451:SF6. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF09180. ProRS-C_1. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SMARTiSM00946. ProRS-C_1. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF64586. SSF64586. 1 hit.
TIGRFAMsiTIGR00408. proS_fam_I. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1AX25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEAVERLTK KSEDSSRWYL ELVRMAKLAD YGPVRGTFAI RPYGFAIWER
60 70 80 90 100
IQADLDARFK ATGHVNAYFP LLIPESYLTK EAEHVEGFAP ECAWVTVGGD
110 120 130 140 150
DELEERLAIR PTSESIICDF YRKWIHSYRD LPVLINQWCN VLRWEMVTRP
160 170 180 190 200
FLRTAEFLWQ EGHTVHATAE EAREEALRML NVYRDCFYEV LAIPVLTGMK
210 220 230 240 250
SPSERFAGAV ETFTCEGLMG DGRALQAATS HDLGQNFARA FDITFLDENQ
260 270 280 290 300
ERVHPYQTSW GFSTRTIGAL ILVHGDDRGL RLPPKLAPTQ AVVVPIWRGK
310 320 330 340 350
NKGEVRREAE ALHRELAEAG LRAEADLDEE HSPGWKFNEH ELRGVPVRVE
360 370 380 390 400
LGPKDIEKGQ AVLVRRDTGE KEFVGRGAAA RRLVELMDEI QQNMLRQAEA
410 420 430 440 450
FRDENTRRAE TYEEFKEIIE EKRGFVVAPW DGTEETEQRI KEETKATIRL
460 470
LPFEREEGKD LVSGRPGKTA VFARAY
Length:476
Mass (Da):54,544
Last modified:July 11, 2006 - v1
Checksum:iFF54D8996514A556
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000386 Genomic DNA. Translation: ABG04053.1.
RefSeqiWP_011564071.1. NC_008148.1.
YP_643865.1. NC_008148.1.

Genome annotation databases

EnsemblBacteriaiABG04053; ABG04053; Rxyl_1087.
KEGGirxy:Rxyl_1087.
PATRICi23369020. VBIRubXyl52678_1115.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000386 Genomic DNA. Translation: ABG04053.1.
RefSeqiWP_011564071.1. NC_008148.1.
YP_643865.1. NC_008148.1.

3D structure databases

ProteinModelPortaliQ1AX25.
SMRiQ1AX25. Positions 6-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266117.Rxyl_1087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG04053; ABG04053; Rxyl_1087.
KEGGirxy:Rxyl_1087.
PATRICi23369020. VBIRubXyl52678_1115.

Phylogenomic databases

eggNOGiCOG0442.
HOGENOMiHOG000167538.
KOiK01881.
OMAiEGFAPEC.
OrthoDBiEOG6G7R2R.

Enzyme and pathway databases

BioCyciRXYL266117:GH8O-1104-MONOMER.

Family and domain databases

Gene3Di3.30.110.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_01571. Pro_tRNA_synth_type3.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004499. Pro-tRNA-ligase_IIa_arc-type.
IPR016061. Pro-tRNA_ligase_II_C.
IPR017449. Pro-tRNA_synth_II.
[Graphical view]
PANTHERiPTHR11451:SF6. PTHR11451:SF6. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF09180. ProRS-C_1. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SMARTiSM00946. ProRS-C_1. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF64586. SSF64586. 1 hit.
TIGRFAMsiTIGR00408. proS_fam_I. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 9941 / NBRC 16129.

Entry informationi

Entry nameiSYP_RUBXD
AccessioniPrimary (citable) accession number: Q1AX25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 11, 2006
Last modified: June 24, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.