Q1AWE0 (RUVB_RUBXD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holliday junction ATP-dependent DNA helicase RuvB
EC=3.6.4.12

Gene names

Name:	ruvB
Ordered Locus Names:	Rxyl_1324

Organism

Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129) [Complete proteome] [HAMAP]

Taxonomic identifier

266117 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Rubrobacteridae › Rubrobacterales › Rubrobacterineae › Rubrobacteraceae › Rubrobacter › Rubrobacter xylanophilus

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing By similarity. HAMAP-Rule MF_00016
Catalytic activity	ATP + H2O = ADP + phosphate. HAMAP-Rule MF_00016
Subunit structure	Forms a complex with RuvA By similarity.
Sequence similarities	Belongs to the RuvB family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro four-way junction helicase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Holliday junction ATP-dependent DNA helicase RuvB HAMAP-Rule MF_00016		PRO_0000322838
Regions
	Nucleotide binding	81 – 88	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1AWE0 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: A0CF64A5074EC4DF	FASTA	355	39,339
	10 20 30 40 50 60 MEEMDDFTVR RGEREDITGA AGPPEERPLD PAAFEEDDEP TLRPRTLDEF VGQERLKENL 70 80 90 100 110 120 RIFVEAAKQR GEPLDHMLLA GPPGLGKTSL CRILAAEMGV QLHPTSGPSL ERAGDMAAIL 130 140 150 160 170 180 TSLEEGDFLF IDEIHRLNRQ IEEVLYPAME DFAIDIVLGQ GPSARTIRMD LPRFTLVGAT 190 200 210 220 230 240 TRTGLMTKPL LDRFGFSARL DYYEPHELEK IVVRNARILG VPITEGGARQ LARRSRGTPR 250 260 270 280 290 300 VANRLLKRVR DYAQVVGDGT IDEETANAAL EMQGVDHLGL DRTDREYLSL IIEKFDGGPV 310 320 330 340 350 GVGTLSVALG EARDTVEDVY EPYLLQSGLI QRTSRGRVAT RHAYAHLGFP VRDGG

References

[1]

"Complete sequence of Rubrobacter xylanophilus DSM 9941." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., da Costa M.S., Rainey F.A., Empadinhas N., Jolivet E., Battista J.R., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 9941 / NBRC 16129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000386 Genomic DNA. Translation: ABG04288.1.
RefSeq	YP_644100.1. NC_008148.1.
3D structure databases
ProteinModelPortal	Q1AWE0.
SMR	Q1AWE0. Positions 42-350.
ModBase	Search...
Protein-protein interaction databases
STRING	266117.Rxyl_1324.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABG04288; ABG04288; Rxyl_1324.
GeneID	4115991.
KEGG	rxy:Rxyl_1324.
PATRIC	23369524. VBIRubXyl52678_1361.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2255.
HOGENOM	HOG000218623.
KO	K03551.
OMA	HIIAKEM.
ProtClustDB	PRK00080.
Enzyme and pathway databases
BioCyc	RXYL266117:GH8O-1388-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
HAMAP	MF_00016. DNA_helic_RuvB.
InterPro	IPR003593. AAA+_ATPase. IPR004605. DNA_helicase_Holl-junc_RuvB. IPR008823. DNA_helicase_Holl-junc_RuvB_C. IPR008824. DNA_helicase_Holl-junc_RuvB_N. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
PANTHER	PTHR13779:SF2. PTHR13779:SF2. 1 hit.
Pfam	PF05491. RuvB_C. 1 hit. PF05496. RuvB_N. 1 hit. [Graphical view]
ProDom	PD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00635. ruvB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUVB_RUBXD

Accession

Primary (citable) accession number: Q1AWE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	July 11, 2006
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program