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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (Rxyl_0960)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70ShikimateUniRule annotation1
Active sitei74Proton acceptorUniRule annotation1
Binding sitei86NADPUniRule annotation1
Binding sitei95ShikimateUniRule annotation1
Binding sitei111ShikimateUniRule annotation1
Binding sitei227NADP; via carbonyl oxygenUniRule annotation1
Binding sitei229ShikimateUniRule annotation1
Binding sitei251NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi135 – 139NADPUniRule annotation5
Nucleotide bindingi159 – 164NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:Rxyl_1362
OrganismiRubrobacter xylanophilus (strain DSM 9941 / NBRC 16129)
Taxonomic identifieri266117 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaRubrobacteriaRubrobacteralesRubrobacteraceaeRubrobacter
Proteomesi
  • UP000006637 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003251601 – 284Shikimate dehydrogenase (NADP(+))Add BLAST284

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi266117.Rxyl_1362.

Structurei

3D structure databases

ProteinModelPortaliQ1AWA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 25Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107V59. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
KOiK00014.
OMAiLNITYPC.
OrthoDBiPOG091H02DY.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1AWA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVPRIDGET SLVGLIGHPV SHSLSPQMHN ASFAALGLNY VYVPLDVPPE
60 70 80 90 100
ELEAGVRGLR ALGFRGFNVT MPHKERVCAL VDRLDEAARI SGAVNTVAVE
110 120 130 140 150
EDGSLLGMNT DGSGFLLACR EAGVEPSGRV ALVAGAGGAA AAVAVALLRA
160 170 180 190 200
GLSELRIANR TPGRALGLRE RLGGLGGRVE VFPLSGLAEA AGGADILVNA
210 220 230 240 250
TYLGMRAGDP LPFPEELLRP GVAVCDAVYR PGKSTALVRA ARRRGLAAVP
260 270 280
GELMLLYQGV EAQRAWTGVD PDVGAMRRAL SGEG
Length:284
Mass (Da):29,345
Last modified:July 11, 2006 - v1
Checksum:iF392BE0562F2CE04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000386 Genomic DNA. Translation: ABG04325.1.
RefSeqiWP_011564342.1. NC_008148.1.

Genome annotation databases

EnsemblBacteriaiABG04325; ABG04325; Rxyl_1362.
KEGGirxy:Rxyl_1362.
PATRICi23369598. VBIRubXyl52678_1398.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000386 Genomic DNA. Translation: ABG04325.1.
RefSeqiWP_011564342.1. NC_008148.1.

3D structure databases

ProteinModelPortaliQ1AWA3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266117.Rxyl_1362.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG04325; ABG04325; Rxyl_1362.
KEGGirxy:Rxyl_1362.
PATRICi23369598. VBIRubXyl52678_1398.

Phylogenomic databases

eggNOGiENOG4107V59. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
KOiK00014.
OMAiLNITYPC.
OrthoDBiPOG091H02DY.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_RUBXD
AccessioniPrimary (citable) accession number: Q1AWA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 11, 2006
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.