ID   FTHS2_RUBXD             Reviewed;         576 AA.
AC   Q1AVP8;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   27-MAR-2024, entry version 91.
DE   RecName: Full=Formate--tetrahydrofolate ligase 2 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase 2 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS 2 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS 2 {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs2 {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=Rxyl_1568;
OS   Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1).
OC   Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales; Rubrobacteraceae;
OC   Rubrobacter.
OX   NCBI_TaxID=266117;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 9941 / NBRC 16129 / PRD-1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., da Costa M.S.,
RA   Rainey F.A., Empadinhas N., Jolivet E., Battista J.R., Richardson P.;
RT   "Complete sequence of Rubrobacter xylanophilus DSM 9941.";
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000386; ABG04530.1; -; Genomic_DNA.
DR   RefSeq; WP_011564547.1; NC_008148.1.
DR   AlphaFoldDB; Q1AVP8; -.
DR   SMR; Q1AVP8; -.
DR   STRING; 266117.Rxyl_1568; -.
DR   KEGG; rxy:Rxyl_1568; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_11; -.
DR   OrthoDB; 9761733at2; -.
DR   PhylomeDB; Q1AVP8; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000006637; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..576
FT                   /note="Formate--tetrahydrofolate ligase 2"
FT                   /id="PRO_0000293055"
FT   BINDING         69..76
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   576 AA;  61555 MW;  60FF8BCC619CD358 CRC64;
     MGEALSNLEI ARGAKLLPIE EVGRSMGLRE ERHLEPYGRH VAKVDLCAIE DLSERPKAKY
     ILVSAITPTP LGEGKTTTTV GLGQAFSHIG KRATIAIRQA SMGPAFGIKG GAAGGGYSQV
     VPMERLNLHL TGDLHAVTEA HNMLAAMIDN HLYHGNGLGI EPHSISWRRV MDVNDRSLRN
     IVIGLGARTD GVPRQSGFDI TAASEVMAIL ALASSLEDLR ERLGRIVIGH DREGNPVSAE
     DVRGAGAMAV ILKEAIKPNL MQTLEGTPAL VHAGPFGNIA TGNSSVVADL IGIRTADYLI
     TEAGFGADMG AERFFNIKCR ISGLEPDAAV VVATVRALKA HSGRYQIKAG APLPEELLEE
     NPQDVLAGAE NLKKQIENIK LHGVPAVVAI NAFPTDHPSE HKAIEEAAKE VGARCAVCRH
     FTEGGKGAVE LARALEETIE ENERERRRGG GGSFRFLYPL EMPLKQKIET IAREVYGAEG
     VEYDAEALRA LEGFERAGFG RLPVCLAKTH LSLSSDPALK GAPRGWKLSV REVRASVGAG
     FIYPICGQMR TMPGLSAHPA AERIDLDGEG NVVGLF
//