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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56S-adenosyl-L-methionineUniRule annotation1
Binding sitei81S-adenosyl-L-methionineUniRule annotation1
Binding sitei108S-adenosyl-L-methionineUniRule annotation1
Active sitei130By similarity1
Binding sitei130S-adenosyl-L-methionineUniRule annotation1
Binding sitei134SubstrateUniRule annotation1
Binding sitei166SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Rxyl_2026
OrganismiRubrobacter xylanophilus (strain DSM 9941 / NBRC 16129)
Taxonomic identifieri266117 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaRubrobacteriaRubrobacteralesRubrobacteraceaeRubrobacter
Proteomesi
  • UP000006637 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002882181 – 223tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST223

Interactioni

Protein-protein interaction databases

STRINGi266117.Rxyl_2026.

Structurei

3D structure databases

ProteinModelPortaliQ1AUF5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000033410.
KOiK03439.
OMAiYICHLAR.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1AUF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRRKLGRMK VRPPDPETAQ RYLLRFTGRQ LYHEAERLPG LSSPELFGDQ
60 70 80 90 100
RPLELDVGCG TGEYICHLAR SDPEANFVGV DLHLKSLHKA IRRAEEANLQ
110 120 130 140 150
NIKFICADFR QMYPLLRPSA LRAVYLHFPD PGIKPRYRKR RLFNERFLEE
160 170 180 190 200
MHRAVVPGGR MSLVTDDEDY FRQMLELIER DGRWRRAHEE PYLTGFDPPV
210 220
KSRFQKMWER RGRTIYRFEL VRP
Length:223
Mass (Da):26,541
Last modified:July 11, 2006 - v1
Checksum:i91609C67B89272C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000386 Genomic DNA. Translation: ABG04973.1.

Genome annotation databases

EnsemblBacteriaiABG04973; ABG04973; Rxyl_2026.
KEGGirxy:Rxyl_2026.
PATRICi23370862. VBIRubXyl52678_2019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000386 Genomic DNA. Translation: ABG04973.1.

3D structure databases

ProteinModelPortaliQ1AUF5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266117.Rxyl_2026.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG04973; ABG04973; Rxyl_2026.
KEGGirxy:Rxyl_2026.
PATRICi23370862. VBIRubXyl52678_2019.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000033410.
KOiK03439.
OMAiYICHLAR.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_RUBXD
AccessioniPrimary (citable) accession number: Q1AUF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 11, 2006
Last modified: November 2, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.