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Protein

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Gene

ispD

Organism
Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation

Catalytic activityi

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. no protein annotated in this organism
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei16 – 161Transition state stabilizerUniRule annotation
Sitei21 – 211Transition state stabilizerUniRule annotation
Sitei151 – 1511Positions MEP for the nucleophilic attackUniRule annotation
Sitei207 – 2071Positions MEP for the nucleophilic attackUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Enzyme and pathway databases

BioCyciRXYL266117:GH8O-2220-MONOMER.
UniPathwayiUPA00056; UER00093.

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseUniRule annotation (EC:2.7.7.60UniRule annotation)
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthaseUniRule annotation
MEP cytidylyltransferaseUniRule annotation
Short name:
MCTUniRule annotation
Gene namesi
Name:ispDUniRule annotation
Ordered Locus Names:Rxyl_2176
OrganismiRubrobacter xylanophilus (strain DSM 9941 / NBRC 16129)
Taxonomic identifieri266117 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaRubrobacteriaRubrobacteralesRubrobacteraceaeRubrobacter
Proteomesi
  • UP000006637 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2312312-C-methyl-D-erythritol 4-phosphate cytidylyltransferasePRO_1000071320Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi266117.Rxyl_2176.

Structurei

3D structure databases

ProteinModelPortaliQ1AU08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE5. Bacteria.
COG1211. LUCA.
HOGENOMiHOG000218563.
KOiK00991.
OMAiPQVFDAD.
OrthoDBiEOG6J48RZ.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD.
InterProiIPR001228. IspD.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00453. ispD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1AU08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGAVALVLA GGSGTRMGRP KQFIELLGRP ALYHTLRAFQ EAREVERIYA
60 70 80 90 100
VGEEERIRDL ASGCGIDKLC GCARPGESRA LSARNGLLLC GEEDGVICLI
110 120 130 140 150
HDGSRCLVTP QLIGRVVRAV EEGADGAIPA VPVPDTIKVA DGERVLKTLD
160 170 180 190 200
RSSLRAAQTP QAFRLGLLRR VFSAPEEVLR EATDDASLVE RAGGEVRLVP
210 220 230
GERTNIKLTS PEDLVLAEAI LAARERRGSR L
Length:231
Mass (Da):24,800
Last modified:July 11, 2006 - v1
Checksum:i29F52DEE1372584D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000386 Genomic DNA. Translation: ABG05120.1.
RefSeqiWP_011565134.1. NC_008148.1.

Genome annotation databases

EnsemblBacteriaiABG05120; ABG05120; Rxyl_2176.
KEGGirxy:Rxyl_2176.
PATRICi23371174. VBIRubXyl52678_2169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000386 Genomic DNA. Translation: ABG05120.1.
RefSeqiWP_011565134.1. NC_008148.1.

3D structure databases

ProteinModelPortaliQ1AU08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266117.Rxyl_2176.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABG05120; ABG05120; Rxyl_2176.
KEGGirxy:Rxyl_2176.
PATRICi23371174. VBIRubXyl52678_2169.

Phylogenomic databases

eggNOGiENOG4105CE5. Bacteria.
COG1211. LUCA.
HOGENOMiHOG000218563.
KOiK00991.
OMAiPQVFDAD.
OrthoDBiEOG6J48RZ.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00093.
BioCyciRXYL266117:GH8O-2220-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD.
InterProiIPR001228. IspD.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00453. ispD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 9941 / NBRC 16129.

Entry informationi

Entry nameiISPD_RUBXD
AccessioniPrimary (citable) accession number: Q1AU08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 11, 2006
Last modified: January 20, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.