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Q1AT13

- LIPA_RUBXD

UniProt

Q1AT13 - LIPA_RUBXD

Protein

Lipoyl synthase

Gene

lipA

Organism
Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (11 Jul 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi89 – 891Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi93 – 931Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi96 – 961Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciRXYL266117:GH8O-2603-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:Rxyl_2548
    OrganismiRubrobacter xylanophilus (strain DSM 9941 / NBRC 16129)
    Taxonomic identifieri266117 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaRubrobacteridaeRubrobacteralesRubrobacterineaeRubrobacteraceaeRubrobacter
    ProteomesiUP000006637: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 321321Lipoyl synthasePRO_0000325306Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi266117.Rxyl_2548.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1AT13.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiHPHIPTK.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q1AT13-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRHRWEDRPV APPPDGRPTE YLPFRQERGR HGRPGWLKAR VPDGPGYREI    50
    KETMRGLSLH TVCEEARCPN IGECWNNRTA TFMILGNVCT RSCGFCAVLT 100
    GRPQELDLEE PYRVADAVKK MGLRHAVITS VNRDELPDGG ASVFAATIRA 150
    IRREVPGCAV EVLTPDFKGD RDAIKTVIDA RPDTFNHNIE TVPRLYPAVR 200
    PQAKYGRSLE VLRYAKELDP GVLTKSGFMV GLGEVEEEIV RTMRDLREHG 250
    VDILTIGQYL RPTENHLPMA RYYTPQEFAR YKKLGLEMGF SHVESGPLVR 300
    SSYHAHEQTE DARRGALGAR G 321
    Length:321
    Mass (Da):36,215
    Last modified:July 11, 2006 - v1
    Checksum:i1645E8F45EE60CE7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000386 Genomic DNA. Translation: ABG05465.1.
    RefSeqiWP_011565474.1. NC_008148.1.
    YP_645277.1. NC_008148.1.

    Genome annotation databases

    EnsemblBacteriaiABG05465; ABG05465; Rxyl_2548.
    GeneIDi4115379.
    KEGGirxy:Rxyl_2548.
    PATRICi23371878. VBIRubXyl52678_2514.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000386 Genomic DNA. Translation: ABG05465.1 .
    RefSeqi WP_011565474.1. NC_008148.1.
    YP_645277.1. NC_008148.1.

    3D structure databases

    ProteinModelPortali Q1AT13.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266117.Rxyl_2548.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABG05465 ; ABG05465 ; Rxyl_2548 .
    GeneIDi 4115379.
    KEGGi rxy:Rxyl_2548.
    PATRICi 23371878. VBIRubXyl52678_2514.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi HPHIPTK.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci RXYL266117:GH8O-2603-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 9941 / NBRC 16129.

    Entry informationi

    Entry nameiLIPA_RUBXD
    AccessioniPrimary (citable) accession number: Q1AT13
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: July 11, 2006
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3