Q1AS71 (ALLB_RUBXD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Allantoinase
EC=3.5.2.5

Alternative name(s):
Allantoin-utilizing enzyme

Gene names

Name:	allB
Ordered Locus Names:	Rxyl_2845

Organism

Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129) [Complete proteome] [HAMAP]

Taxonomic identifier

266117 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Rubrobacteridae › Rubrobacterales › Rubrobacterineae › Rubrobacteraceae › Rubrobacter › Rubrobacter xylanophilus

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of allantoin (5-ureidohydantoin) to allantoic acid by hydrolytic cleavage of the five-member hydantoin ring By similarity. HAMAP-Rule MF_01645
Catalytic activity	(S)-allantoin + H2O = allantoate. HAMAP-Rule MF_01645
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Nitrogen metabolism; (S)-allantoin degradation; allantoate from (S)-allantoin: step 1/1. HAMAP-Rule MF_01645
Subunit structure	Homotetramer By similarity.
Post-translational modification	Carbamylation allows a single lysine to coordinate two zinc ions By similarity. HAMAP-Rule MF_01645
Sequence similarities	Belongs to the DHOase family. Allantoinase subfamily.

Ontologies

Keywords
Biological process	Purine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	allantoin catabolic process Inferred from electronic annotation. Source: HAMAP purine nucleobase metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	allantoinase activity Inferred from electronic annotation. Source: HAMAP cobalt ion binding Inferred from electronic annotation. Source: InterPro dihydroorotase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Allantoinase HAMAP-Rule MF_01645		PRO_0000317681
Sites
	Metal binding	60	1	Zinc 1 By similarity
	Metal binding	62	1	Zinc 1 By similarity
	Metal binding	147	1	Zinc 1; via carbamate group By similarity
	Metal binding	147	1	Zinc 2; via carbamate group By similarity
	Metal binding	183	1	Zinc 2 By similarity
	Metal binding	239	1	Zinc 2 By similarity
	Metal binding	312	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	147	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1AS71 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 49772AD22B3E7307	FASTA	454	48,161
	10 20 30 40 50 60 MSAFDLIVRG GTVVRGGCEV ADVGVADGVI SAVGPDLEGG AREEIDARGL LVLPGAIDAH 70 80 90 100 110 120 VHFNEPGRTR WEGFASGTRA LAAGGTTLCV EMPLNAHPPT TDGESFDLKL AAAKASAHVD 130 140 150 160 170 180 FALWGGIVPG RVGRMEELAG RGVAGFKAFM SATGTPDFEA ADDLTLYEGM QEAARLGLPV 190 200 210 220 230 240 LVHAENRQIT DALARRATSA LRTTMRDYLA SRPAVAELEA IGRAILLASE AGCSLHIVHV 250 260 270 280 290 300 STGRGVALVA AARERGVDVT CETCPHYLLF TDEDAVRIGA AAKCAPPLRP REEVESLWEQ 310 320 330 340 350 360 VLAGNVAFVT SDHSPCPPDM KAGEDMFRAW GGISGCQSLL PALLDEGYHG RGLPPERLAE 370 380 390 400 410 420 LLSANVARRF GFAGKGGIEE GNDADLALVD PSGEHVLREE DLHYRHPISP YVGRSFRGRV 430 440 450 VRTILRGRTV FEGGRVVSGP AGRFVRPQRE KGER

References

[1]

"Complete sequence of Rubrobacter xylanophilus DSM 9941." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., da Costa M.S., Rainey F.A., Empadinhas N., Jolivet E., Battista J.R., Richardson P. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 9941 / NBRC 16129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000386 Genomic DNA. Translation: ABG05757.1.
RefSeq	YP_645569.1. NC_008148.1.
3D structure databases
ProteinModelPortal	Q1AS71.
ModBase	Search...
Protein-protein interaction databases
STRING	266117.Rxyl_2845.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABG05757; ABG05757; Rxyl_2845.
GeneID	4115822.
KEGG	rxy:Rxyl_2845.
PATRIC	23372490. VBIRubXyl52678_2817.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0044.
HOGENOM	HOG000219146.
KO	K01466.
OMA	FNEPGRE.
Enzyme and pathway databases
BioCyc	RXYL266117:GH8O-2909-MONOMER.
UniPathway	UPA00395; UER00653.
Family and domain databases
HAMAP	MF_01645. Hydantoinase.
InterPro	IPR017593. Allantoinase. IPR004722. DHOase. IPR002195. Dihydroorotase_CS. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR03178. allantoinase. 1 hit. TIGR00857. pyrC_multi. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALLB_RUBXD

Accession

Primary (citable) accession number: Q1AS71

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 11, 2006
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program