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Protein

Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Gene

Asap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. Plays a role in ciliogenesis (By similarity).By similarity

Enzyme regulationi

Activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri466 – 48924C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
Alternative name(s):
130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein
ADP-ribosylation factor-directed GTPase-activating protein 1
Short name:
ARF GTPase-activating protein 1
Development and differentiation-enhancing factor 1
Short name:
DEF-1
Short name:
Differentiation-enhancing factor 1
PIP2-dependent ARF1 GAP
Gene namesi
Name:Asap1
Synonyms:Ddef1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1307379. Asap1.

Subcellular locationi

  • Cytoplasm
  • Membrane

  • Note: Predominantly cytoplasmic. Partially membrane-associated (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11441144Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1PRO_0000263054Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei308 – 3081Phosphotyrosine; by FAK2By similarity
Modified residuei729 – 7291PhosphoserineCombined sources
Modified residuei738 – 7381PhosphoserineCombined sources
Modified residuei851 – 8511PhosphoserineCombined sources
Modified residuei855 – 8551PhosphoserineBy similarity
Modified residuei1023 – 10231PhosphoserineBy similarity
Modified residuei1042 – 10421PhosphoserineCombined sources
Modified residuei1056 – 10561PhosphoserineCombined sources
Modified residuei1063 – 10631PhosphothreonineBy similarity
Modified residuei1143 – 11431PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated on tyrosine residues by SRC.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ1AAU6.
PRIDEiQ1AAU6.

PTM databases

iPTMnetiQ1AAU6.
PhosphoSiteiQ1AAU6.

Interactioni

Subunit structurei

Homodimer. Interacts with SRC and CRK. Interacts with RAB11FIP3. Interacts with PTK2B/PYK2. Interacts with CTTN. Interacts (via SH3 domain) with APC (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000059763.

Structurei

3D structure databases

ProteinModelPortaliQ1AAU6.
SMRiQ1AAU6. Positions 338-440, 449-721, 1082-1144.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini336 – 42893PHPROSITE-ProRule annotationAdd
BLAST
Domaini451 – 574124Arf-GAPPROSITE-ProRule annotationAdd
BLAST
Repeati612 – 64433ANK 1Add
BLAST
Repeati648 – 67730ANK 2Add
BLAST
Domaini1082 – 114463SH3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi795 – 1008214Pro-richAdd
BLAST

Domaini

The PH domain most probably contributes to the phosphoinositide-dependent regulation of ADP ribosylation factors.By similarity

Sequence similaritiesi

Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri466 – 48924C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

ANK repeat, Repeat, SH3 domain, Zinc-finger

Phylogenomic databases

eggNOGiKOG0521. Eukaryota.
COG5347. LUCA.
HOGENOMiHOG000230570.
HOVERGENiHBG051327.
InParanoidiQ1AAU6.
KOiK12488.
PhylomeDBiQ1AAU6.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q1AAU6-1) [UniParc]FASTAAdd to basket

Also known as: Variant a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSSASRLSS FSSRDSLWNR MPDQISVSEF IAETTEDYNS PTTSSFTTRL
60 70 80 90 100
HNCRNTVTLL EEALDQDRTA LQKVKKSVKA IYNSGQDHVQ NEENYAQVLD
110 120 130 140 150
KFGSNFLSRD NPDLGTAFVK FSTLTKELST LLKNLLQGLS HNVIFTLDSL
160 170 180 190 200
LKGDLKGVKG DLKKPFDKAW KDYEMKFTKI EKEKREHAKQ HGMIRTEITG
210 220 230 240 250
AEIAEEMEKE RRLFQLQMCE YLIKVNEIKT KKGVDLLQNL IKYYHAQCNF
260 270 280 290 300
FQDGLKTADK LKQYIEKLAA DLYNIKQTQD EEKKQLTALR DLIKSSLQLD
310 320 330 340 350
PKEVGGLYVP SRANSDSQSR QGGYSMHQLQ GNKEYGSEKK GFLLKKSDGI
360 370 380 390 400
RKVWQRRKCA VKNGILTISH ATSNRQPAKL NLLTCQVKPN AEDKKSFDLI
410 420 430 440 450
SHNRTYHFQA EDEQDYVAWI SVLTNSKEEA LTMAFRGEQS TGENSLEDLT
460 470 480 490 500
KAIIEDVQRL PGNDICCDCG SSEPTWLSTN LGILTCIECS GIHREMGVHI
510 520 530 540 550
SRIQSLELDK LGTSELLLAK NVGNNSFNDI MEANLPSPSP KPTPSSDMTV
560 570 580 590 600
RKEYITAKYV DHRFSRKTCA SSSAKLNELL EAIKSRDLLA LIQVYAEGVE
610 620 630 640 650
LMEPLLEPGQ ELGETALHLA VRTADQTSLH LVDFLVQNCG NLDKQTSVGN
660 670 680 690 700
TVLHYCSMYG KPECLKLLLR SKPTVDIVNQ NGETALDIAK RLKATQCEDL
710 720 730 740 750
LSQAKSGKFN PHVHVEYEWN LRQDEMDESD DDLDDKPSPI KKERSPRPQS
760 770 780 790 800
FCHSSSISPQ DKLALPGFST PRDKQRLSYG AFTNQIFVST STDLPTSPTS
810 820 830 840 850
EAPPLPPRNA GKGPTGPPST LPLGTQTSSG SSTLSKKRSP PPPPGHKRTL
860 870 880 890 900
SDPPSPLPHG PPNKGAIPWG NDVGPSSSSK TANKFEGLSQ QASTSSAKTA
910 920 930 940 950
LGPRVLPKLP QKVALRKTET SHHLSLDRAN IPPETFQKSS QLSELPQKPP
960 970 980 990 1000
LGDLPPKPME LAPKPQIGEL PPKPGELPPK PQLGDLPPKP QLSDLPPKPQ
1010 1020 1030 1040 1050
MKDLPPKPQL GDLLAKSQAS DLSAKVQPPS EVTQRSHTGD LSPNVQSRDA
1060 1070 1080 1090 1100
IQKQASEDSN DLTPTLPETP VPLPRKINTG KNKVRRVKTI YDCQADNDDE
1110 1120 1130 1140
LTFIEGEVII VTGEEDQEWW IGHIEGQPER KGVFPVSFVH ILSD
Length:1,144
Mass (Da):127,088
Last modified:December 12, 2006 - v2
Checksum:i798B02B3194BBA38
GO
Isoform 2 (identifier: Q1AAU6-2) [UniParc]FASTAAdd to basket

Also known as: Variant b

The sequence of this isoform differs from the canonical sequence as follows:
     813-869: Missing.

Show »
Length:1,087
Mass (Da):121,351
Checksum:i7D5666D4702A3D63
GO
Isoform 3 (identifier: Q1AAU6-3) [UniParc]FASTAAdd to basket

Also known as: Variant c

The sequence of this isoform differs from the canonical sequence as follows:
     304-315: Missing.
     813-869: Missing.

Show »
Length:1,075
Mass (Da):120,150
Checksum:i53F4C848913B85BF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti175 – 1751M → T in ABB71897 (Ref. 1) Curated
Sequence conflicti175 – 1751M → T in ABB71898 (Ref. 1) Curated
Sequence conflicti418 – 4181A → T in ABB71896 (Ref. 1) Curated
Sequence conflicti520 – 5201K → Q in ABB71897 (Ref. 1) Curated
Sequence conflicti520 – 5201K → Q in ABB71898 (Ref. 1) Curated
Sequence conflicti1092 – 10921D → N in ABB71897 (Ref. 1) Curated
Sequence conflicti1092 – 10921D → N in ABB71898 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei304 – 31512Missing in isoform 3. 1 PublicationVSP_021850Add
BLAST
Alternative sequencei813 – 86957Missing in isoform 2 and isoform 3. 1 PublicationVSP_021851Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ238622 mRNA. Translation: ABB71896.1.
DQ238623 mRNA. Translation: ABB71897.1.
DQ238624 mRNA. Translation: ABB71898.1.
RefSeqiNP_001037710.1. NM_001044245.1.
UniGeneiRn.63466.

Genome annotation databases

GeneIDi314961.
KEGGirno:314961.
UCSCiRGD:1307379. rat. [Q1AAU6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ238622 mRNA. Translation: ABB71896.1.
DQ238623 mRNA. Translation: ABB71897.1.
DQ238624 mRNA. Translation: ABB71898.1.
RefSeqiNP_001037710.1. NM_001044245.1.
UniGeneiRn.63466.

3D structure databases

ProteinModelPortaliQ1AAU6.
SMRiQ1AAU6. Positions 338-440, 449-721, 1082-1144.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000059763.

PTM databases

iPTMnetiQ1AAU6.
PhosphoSiteiQ1AAU6.

Proteomic databases

PaxDbiQ1AAU6.
PRIDEiQ1AAU6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi314961.
KEGGirno:314961.
UCSCiRGD:1307379. rat. [Q1AAU6-1]

Organism-specific databases

CTDi50807.
RGDi1307379. Asap1.

Phylogenomic databases

eggNOGiKOG0521. Eukaryota.
COG5347. LUCA.
HOGENOMiHOG000230570.
HOVERGENiHBG051327.
InParanoidiQ1AAU6.
KOiK12488.
PhylomeDBiQ1AAU6.

Miscellaneous databases

PROiQ1AAU6.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Enhanced ASAP1 expression is associated with tumor development and progression, and promotes metastasis in experimental tumors."
    Mueller T., Rothley M., Bauer M., Pankraz M., Sleeman J.
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: Sprague-Dawley.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-729; SER-738; SER-851; SER-1042; SER-1056 AND SER-1143, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiASAP1_RAT
AccessioniPrimary (citable) accession number: Q1AAU6
Secondary accession number(s): Q1AAU4, Q1AAU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: June 8, 2016
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.