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Protein

Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Gene

Asap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. Plays a role in ciliogenesis (By similarity).By similarity

Enzyme regulationi

Activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri466 – 489C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
Biological processCilium biogenesis/degradation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
Alternative name(s):
130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein
ADP-ribosylation factor-directed GTPase-activating protein 1
Short name:
ARF GTPase-activating protein 1
Development and differentiation-enhancing factor 1
Short name:
DEF-1
Short name:
Differentiation-enhancing factor 1
PIP2-dependent ARF1 GAP
Gene namesi
Name:Asap1
Synonyms:Ddef1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1307379 Asap1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002630541 – 1144Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1Add BLAST1144

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei308Phosphotyrosine; by FAK2By similarity1
Modified residuei729PhosphoserineCombined sources1
Modified residuei738PhosphoserineCombined sources1
Modified residuei851PhosphoserineCombined sources1
Modified residuei855PhosphoserineBy similarity1
Modified residuei1023PhosphoserineBy similarity1
Modified residuei1042PhosphoserineCombined sources1
Modified residuei1056PhosphoserineCombined sources1
Modified residuei1063PhosphothreonineBy similarity1
Modified residuei1143PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on tyrosine residues by SRC.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ1AAU6
PRIDEiQ1AAU6

PTM databases

iPTMnetiQ1AAU6
PhosphoSitePlusiQ1AAU6

Interactioni

Subunit structurei

Homodimer. Interacts with SRC and CRK. Interacts with RAB11FIP3. Interacts with PTK2B/PYK2. Interacts with CTTN. Interacts (via SH3 domain) with APC (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000059763

Structurei

3D structure databases

ProteinModelPortaliQ1AAU6
SMRiQ1AAU6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini336 – 428PHPROSITE-ProRule annotationAdd BLAST93
Domaini451 – 574Arf-GAPPROSITE-ProRule annotationAdd BLAST124
Repeati612 – 644ANK 1Add BLAST33
Repeati648 – 677ANK 2Add BLAST30
Domaini1082 – 1144SH3PROSITE-ProRule annotationAdd BLAST63

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi795 – 1008Pro-richAdd BLAST214

Domaini

The PH domain most probably contributes to the phosphoinositide-dependent regulation of ADP ribosylation factors.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri466 – 489C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Repeat, SH3 domain, Zinc-finger

Phylogenomic databases

eggNOGiKOG0521 Eukaryota
COG5347 LUCA
HOGENOMiHOG000230570
HOVERGENiHBG051327
InParanoidiQ1AAU6
KOiK12488
PhylomeDBiQ1AAU6

Family and domain databases

CDDicd00204 ANK, 1 hit
cd07641 BAR_ASAP1, 1 hit
cd13251 PH_ASAP, 1 hit
cd11965 SH3_ASAP1, 1 hit
Gene3Di1.20.1270.60, 1 hit
2.30.29.30, 1 hit
3.30.40.160, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR037278 ARFGAP/RecO
IPR001164 ArfGAP_dom
IPR038508 ArfGAP_dom_sf
IPR037928 ASAP1_BAR
IPR038016 ASAP1_SH3
IPR011993 PH-like_dom_sf
IPR037844 PH_ASAP
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF01412 ArfGap, 1 hit
PF00169 PH, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00405 REVINTRACTNG
SMARTiView protein in SMART
SM00248 ANK, 2 hits
SM00105 ArfGap, 1 hit
SM00233 PH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 2 hits
SSF48403 SSF48403, 1 hit
SSF50044 SSF50044, 1 hit
SSF57863 SSF57863, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 2 hits
PS50115 ARFGAP, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50002 SH3, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q1AAU6-1) [UniParc]FASTAAdd to basket
Also known as: Variant a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSSASRLSS FSSRDSLWNR MPDQISVSEF IAETTEDYNS PTTSSFTTRL
60 70 80 90 100
HNCRNTVTLL EEALDQDRTA LQKVKKSVKA IYNSGQDHVQ NEENYAQVLD
110 120 130 140 150
KFGSNFLSRD NPDLGTAFVK FSTLTKELST LLKNLLQGLS HNVIFTLDSL
160 170 180 190 200
LKGDLKGVKG DLKKPFDKAW KDYEMKFTKI EKEKREHAKQ HGMIRTEITG
210 220 230 240 250
AEIAEEMEKE RRLFQLQMCE YLIKVNEIKT KKGVDLLQNL IKYYHAQCNF
260 270 280 290 300
FQDGLKTADK LKQYIEKLAA DLYNIKQTQD EEKKQLTALR DLIKSSLQLD
310 320 330 340 350
PKEVGGLYVP SRANSDSQSR QGGYSMHQLQ GNKEYGSEKK GFLLKKSDGI
360 370 380 390 400
RKVWQRRKCA VKNGILTISH ATSNRQPAKL NLLTCQVKPN AEDKKSFDLI
410 420 430 440 450
SHNRTYHFQA EDEQDYVAWI SVLTNSKEEA LTMAFRGEQS TGENSLEDLT
460 470 480 490 500
KAIIEDVQRL PGNDICCDCG SSEPTWLSTN LGILTCIECS GIHREMGVHI
510 520 530 540 550
SRIQSLELDK LGTSELLLAK NVGNNSFNDI MEANLPSPSP KPTPSSDMTV
560 570 580 590 600
RKEYITAKYV DHRFSRKTCA SSSAKLNELL EAIKSRDLLA LIQVYAEGVE
610 620 630 640 650
LMEPLLEPGQ ELGETALHLA VRTADQTSLH LVDFLVQNCG NLDKQTSVGN
660 670 680 690 700
TVLHYCSMYG KPECLKLLLR SKPTVDIVNQ NGETALDIAK RLKATQCEDL
710 720 730 740 750
LSQAKSGKFN PHVHVEYEWN LRQDEMDESD DDLDDKPSPI KKERSPRPQS
760 770 780 790 800
FCHSSSISPQ DKLALPGFST PRDKQRLSYG AFTNQIFVST STDLPTSPTS
810 820 830 840 850
EAPPLPPRNA GKGPTGPPST LPLGTQTSSG SSTLSKKRSP PPPPGHKRTL
860 870 880 890 900
SDPPSPLPHG PPNKGAIPWG NDVGPSSSSK TANKFEGLSQ QASTSSAKTA
910 920 930 940 950
LGPRVLPKLP QKVALRKTET SHHLSLDRAN IPPETFQKSS QLSELPQKPP
960 970 980 990 1000
LGDLPPKPME LAPKPQIGEL PPKPGELPPK PQLGDLPPKP QLSDLPPKPQ
1010 1020 1030 1040 1050
MKDLPPKPQL GDLLAKSQAS DLSAKVQPPS EVTQRSHTGD LSPNVQSRDA
1060 1070 1080 1090 1100
IQKQASEDSN DLTPTLPETP VPLPRKINTG KNKVRRVKTI YDCQADNDDE
1110 1120 1130 1140
LTFIEGEVII VTGEEDQEWW IGHIEGQPER KGVFPVSFVH ILSD
Length:1,144
Mass (Da):127,088
Last modified:December 12, 2006 - v2
Checksum:i798B02B3194BBA38
GO
Isoform 2 (identifier: Q1AAU6-2) [UniParc]FASTAAdd to basket
Also known as: Variant b

The sequence of this isoform differs from the canonical sequence as follows:
     813-869: Missing.

Show »
Length:1,087
Mass (Da):121,351
Checksum:i7D5666D4702A3D63
GO
Isoform 3 (identifier: Q1AAU6-3) [UniParc]FASTAAdd to basket
Also known as: Variant c

The sequence of this isoform differs from the canonical sequence as follows:
     304-315: Missing.
     813-869: Missing.

Show »
Length:1,075
Mass (Da):120,150
Checksum:i53F4C848913B85BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti175M → T in ABB71897 (Ref. 1) Curated1
Sequence conflicti175M → T in ABB71898 (Ref. 1) Curated1
Sequence conflicti418A → T in ABB71896 (Ref. 1) Curated1
Sequence conflicti520K → Q in ABB71897 (Ref. 1) Curated1
Sequence conflicti520K → Q in ABB71898 (Ref. 1) Curated1
Sequence conflicti1092D → N in ABB71897 (Ref. 1) Curated1
Sequence conflicti1092D → N in ABB71898 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021850304 – 315Missing in isoform 3. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_021851813 – 869Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ238622 mRNA Translation: ABB71896.1
DQ238623 mRNA Translation: ABB71897.1
DQ238624 mRNA Translation: ABB71898.1
RefSeqiNP_001037710.1, NM_001044245.1
UniGeneiRn.63466

Genome annotation databases

GeneIDi314961
KEGGirno:314961
UCSCiRGD:1307379 rat [Q1AAU6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiASAP1_RAT
AccessioniPrimary (citable) accession number: Q1AAU6
Secondary accession number(s): Q1AAU4, Q1AAU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: May 23, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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