Reviewed, UniProtKB/Swiss-Prot Q197F1 (RPB2_IIV3)

Names and origin

Protein names

Recommended name:
    Probable DNA-directed RNA polymerase II subunit RPB2 homolog
    EC=2.7.7.6

Gene names

ORF Names:

IIV3-009R

Organism

Invertebrate iridescent virus 3 (IIV-3) (Mosquito iridescent virus)

Taxonomic identifier

345201 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Iridoviridae › Chloriridovirus

Virus host

Aedes vexans (Inland floodwater mosquito) (Culex vexans) [TaxID: 7163]
Psorophora ferox [TaxID: 7183]
Culex territans [TaxID: 42431]
Ochlerotatus sollicitans (eastern saltmarsh mosquito) [TaxID: 310513]
Ochlerotatus taeniorhynchus (Black salt marsh mosquito) (Aedes taeniorhynchus) [TaxID: 329105]
Culiseta annulata [TaxID: 332058]

Protein attributes

Sequence length	1140 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Domain	Zinc-finger
Ligand	Magnesium Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Virus reference strain
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1140	1140	Probable DNA-directed RNA polymerase II subunit RPB2 homolog		PRO_0000377754
Regions
	Zinc finger	1092 – 1108	17	C4-type Potential
Sites
	Metal binding	773	1	Magnesium; shared with DNA-directed RNA polymerase largest subunit By similarity
	Metal binding	1092	1	Zinc By similarity
	Metal binding	1095	1	Zinc By similarity
	Metal binding	1105	1	Zinc By similarity
	Metal binding	1108	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q197F1-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 5AF25A307D89BFB8	FASTA	1,140	128,256
	10 20 30 40 50 60 MNQLNQKTKL DKHLSSDNRA KIVADYFKTN GLVKHQIETF NHFISKGLKT IINNESSIKY 70 80 90 100 110 120 QSHDNYSLKF TNIYVEYPSI IDDDRTIRDL YPQEARNKDL SYTGNVCVNV IEMVENKEGK 130 140 150 160 170 180 PPQISEQYRV PIAKIPIMLG SDVCHLSKYT PLENEQINGH SQADQGGYFI INGKERVLIS 190 200 210 220 230 240 QVRKAYNKPV CFVKSTSQKE DVLICEMRSM CEETFHSTSV QVKMIKNKIM VSLKLKRKLI 250 260 270 280 290 300 DIPVGIVFKC LGYNPDQMGS KFRSLFNLPP EYEKYIDAIK NDCLEEFAYG IAVSAADGDN 310 320 330 340 350 360 SDSGGESGGE EEADQEEHIV KVFRNMSTSV DKDGGMAATG ITIEDVQKSL DMDLFPHLGI 370 380 390 400 410 420 TSTRKQRVDL LAFMVKKYLL TLTRKIPVDN RDDYNHKRVE TAGELYSFLF RLLYKKFQKT 430 440 450 460 470 480 CITQIRNRKP DISNFLRTSG ITTGILYSFS SGYWGVQRNT YIRTGVSQVV NPKVSLLANY 490 500 510 520 530 540 SSLRRVVIPE SKDGKEAKTS EIRQIHPSSS FLVCPVETPE GKGVGTVLNM AVFCQVTTGI 550 560 570 580 590 600 STCEIMDQID SFGVDLKCHC TIKDPNNCLL LLINGSPYGN GHANVIHRLN FLNDTNISIV 610 620 630 640 650 660 VNRALGVIEI FSDAGRFIRP IFDLDKICNF EGEIVPSFKW FLDNQLIRYI DINEAAANSI 670 680 690 700 710 720 AIELRDLSTN PNTRYNLMEL DPCGMFGIVA GIIPFPDRTQ SARNCFYSSM VKQAIGFVPC 730 740 750 760 770 780 HNLKTETVSH TLNYPQKPLV TTSFAEYNQL NEYPNGINAI VAIACYTGYN QEDSIILNKS 790 800 810 820 830 840 SIDRGLFGTI TYNTFTAQEK KNGIIEERIE IPSNAIKIRD CNYGLVGPDG IVRLRQRVKK 850 860 870 880 890 900 GDVLICKVTI KNKNQDEKLV DSSVIVQHGE EGYVDRIVDN VIDGCRIVKV VIGQMRTPEI 910 920 930 940 950 960 GDKFCSGMAQ KGTCGMIFPQ EDMPFTASGM TPDIIINPNC IPSRMTINQI ISTVMGKLYT 970 980 990 1000 1010 1020 VNPNPRFRNG NSFQENSNTI LKELCHHLKL NGFDPSGSEV MYSGFTGERI QSTIFMGPTY 1030 1040 1050 1060 1070 1080 YHRLKHMVKD KMHARSHGQV TTLHRQPNCG RSQGGGLRFG EMEKDCILVH GATQFLNERM 1090 1100 1110 1120 1130 1140 FLNSDPFQID VCKDCGMMSS TSKKCHHCGS INVKRCNIPY SCKNLLQELN GMGIKTKIDL

References

[1]	"Genome of invertebrate iridescent virus type 3 (mosquito iridescent virus)." Delhon G., Tulman E.R., Afonso C.L., Lu Z., Becnel J.J., Moser B.A., Kutish G.F., Rock D.L. J. Virol. 80:8439-8449(2006) [PubMed: 16912294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ643392 Genomic DNA. Translation: ABF82039.1.
RefSeq	YP_654581.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4156258.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007644. RNA_pol_bsu_protrusion. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. [Graphical view]
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPB2_IIV3

Accession

Primary (citable) accession number: Q197F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	July 11, 2006
Last modified:	September 22, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)