Q197B2 (RIR2_IIV3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ribonucleoside-diphosphate reductase small subunit 048L
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase small subunit

Gene names

ORF Names:

IIV3-048L

Organism

Invertebrate iridescent virus 3 (IIV-3) (Mosquito iridescent virus) [Reference proteome]

Taxonomic identifier

345201 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Iridoviridae › Chloriridovirus

Virus host

Aedes vexans (Inland floodwater mosquito) (Culex vexans) [TaxID: 7163]
Culex territans [TaxID: 42431]
Culiseta annulata [TaxID: 332058]
Ochlerotatus sollicitans (eastern saltmarsh mosquito) [TaxID: 310513]
Ochlerotatus taeniorhynchus (Black salt marsh mosquito) (Aedes taeniorhynchus) [TaxID: 329105]
Psorophora ferox [TaxID: 7183]

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Probable ribonucleoside-diphosphate reductase small subunit 048L		PRO_0000376909
Sites
	Active site	147	1	By similarity
	Metal binding	110	1	Iron 1 By similarity
	Metal binding	140	1	Iron 1 By similarity
	Metal binding	140	1	Iron 2 By similarity
	Metal binding	143	1	Iron 1 By similarity
	Metal binding	217	1	Iron 2 By similarity
	Metal binding	251	1	Iron 2 By similarity
	Metal binding	254	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q197B2 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 8D3716EF3DA19D30	FASTA	376	44,070
	10 20 30 40 50 60 MEYEHLWLTA YGCLAVVGAV VVYCAIKQSL VTNWWFYGSI YPQVKLMADR TTFKPFEYPG 70 80 90 100 110 120 CYDKWDTHEH AHWSFKELNM QDDVHDWHNR LSEAEKTFLV QILRFFTQGD VDVAVGYVQY 130 140 150 160 170 180 LQLFQQPEVR MMLFSFGARE AMHIASYSHL ISTLNLPDVT YQEFLKYKAM KDKHEFVFNS 190 200 210 220 230 240 RFKSTTVGRF FNYLLFGYDT HLEEIAVKIA LFSAFIEGVQ LFSSFIMLLN FTRHGLMKKM 250 260 270 280 290 300 GQIIQWSIAD ETHHTNSMME LFSTLVVENK SHIRLGVLEA RVRDTARKIV QLEDGFIDLA 310 320 330 340 350 360 FSMGEMRQLT ADDVKSYIRY ITNRRLQTMG YLPLYSVVEN PLPWVEDLLN APSHTNFFEN 370 KPTEYAKASL TGDWPW

References

[1]	"Genome of invertebrate iridescent virus type 3 (mosquito iridescent virus)." Delhon G., Tulman E.R., Afonso C.L., Lu Z., Becnel J.J., Moser B.A., Kutish G.F., Rock D.L. J. Virol. 80:8439-8449(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ643392 Genomic DNA. Translation: ABF82078.1.
RefSeq	YP_654620.1. NC_008187.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4156298.
Phylogenomic databases
ProtClustDB	CLSP2511500.
Enzyme and pathway databases
UniPathway	UPA00326.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR000358. RNR_small. [Graphical view]
PANTHER	PTHR23409. PTHR23409. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00368. RIBORED_SMALL. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2_IIV3

Accession

Primary (citable) accession number: Q197B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	July 11, 2006
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program