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Protein

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

Gene

dlat-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).Curated

Catalytic activityi

Acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine.Curated

Cofactori

(R)-lipoateBy similarityNote: Binds 1 lipoyl cofactor covalently.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei480Sequence analysis1
Active sitei484Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processGlycolysis

Enzyme and pathway databases

ReactomeiR-CEL-204174 Regulation of pyruvate dehydrogenase (PDH) complex
R-CEL-389661 Glyoxylate metabolism and glycine degradation
R-CEL-5362517 Signaling by Retinoic Acid
R-CEL-70268 Pyruvate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (EC:2.3.1.12)
Alternative name(s):
Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
Pyruvate dehydrogenase complex component E2
Short name:
PDC-E2
Short name:
PDCE2
Gene namesi
Name:dlat-1Imported
ORF Names:F23B12.5Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiF23B12.5 ; CE09597 ; WBGene00009082 ; dlat-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000244484? – 507Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei118N6-lipoyllysinePROSITE-ProRule annotationBy similarity1

Proteomic databases

EPDiQ19749
PaxDbiQ19749
PeptideAtlasiQ19749
PRIDEiQ19749

2D gel databases

World-2DPAGEi0020:Q19749

Expressioni

Gene expression databases

BgeeiWBGene00009082

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
lit-1Q9U9Y82EBI-320763,EBI-318513

Protein-protein interaction databases

BioGridi44949, 17 interactors
DIPiDIP-24773N
IntActiQ19749, 22 interactors
STRINGi6239.F23B12.5.2

Structurei

3D structure databases

ProteinModelPortaliQ19749
SMRiQ19749
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 153Lipoyl-bindingPROSITE-ProRule annotationAdd BLAST77
Domaini221 – 258Peripheral subunit-binding (PSBD)PROSITE-ProRule annotationAdd BLAST38

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.Sequence analysis

Keywords - Domaini

Lipoyl, Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0557 Eukaryota
COG0508 LUCA
GeneTreeiENSGT00890000139393
HOGENOMiHOG000281566
InParanoidiQ19749
KOiK00627
OMAiTMEFESF
OrthoDBiEOG091G0CAV
PhylomeDBiQ19749

Family and domain databases

Gene3Di3.30.559.10, 1 hit
4.10.320.10, 1 hit
InterProiView protein in InterPro
IPR003016 2-oxoA_DH_lipoyl-BS
IPR001078 2-oxoacid_DH_actylTfrase
IPR000089 Biotin_lipoyl
IPR023213 CAT-like_dom_sf
IPR036625 E3-bd_dom_sf
IPR006257 LAT1
IPR004167 PSBD
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF00198 2-oxoacid_dh, 1 hit
PF00364 Biotin_lipoyl, 1 hit
PF02817 E3_binding, 1 hit
SUPFAMiSSF47005 SSF47005, 1 hit
SSF51230 SSF51230, 1 hit
TIGRFAMsiTIGR01349 PDHac_trf_mito, 1 hit
PROSITEiView protein in PROSITE
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00189 LIPOYL, 1 hit
PS51826 PSBD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q19749-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKFPVPLRT IGGLRPSTTA AISAANIGFT QSSRALSTGA AAKSSGLVGQ
60 70 80 90 100
VARQYPNAAA FSIKQVRLYS SGNLPKHNRV ALPALSPTME LGTVVSWQKK
110 120 130 140 150
EGDQLSEGDL LCEIETDKAT MGFETPEEGY LAKILIQEGS KDVPIGKLLC
160 170 180 190 200
IIVDNEADVA AFKDFKDDGA SSGGSAPAAE KAPEPAKPAA SSQPSPPAQM
210 220 230 240 250
YQAPSVPKSA PIPHSSSGRV SASPFAKKLA AENGLDLSGV SGSGPGGRIL
260 270 280 290 300
ASDLSQAPAK GATSTTTQAV SGQDYTDIPL SNMRKTIAKR LTESKSTIPH
310 320 330 340 350
YYLTSEIQLD TLLQVREKLN GLLAKGTSGQ ATKISINDFI IKASALACQR
360 370 380 390 400
VPEANSYWMD SFIRENHHVD VSVAVSTPAG LITPIIFNAH AKGLATIASE
410 420 430 440 450
IVELAQRARE GKLQPHEFQG GTFTVSNLGM FGSVSDFTAI INPPQSCILA
460 470 480 490 500
IGGASDKLVP DEAEGYKKIK TMKVTLSCDH RTVDGAVGAV WLRHFKEFLE

KPHTMLL
Length:507
Mass (Da):53,467
Last modified:November 1, 1996 - v1
Checksum:i2C546DA3828C5B09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z77659 Genomic DNA Translation: CAB01163.1
PIRiT21287
RefSeqiNP_506579.1, NM_074178.6
UniGeneiCel.17215

Genome annotation databases

EnsemblMetazoaiF23B12.5.1; F23B12.5.1; WBGene00009082
F23B12.5.2; F23B12.5.2; WBGene00009082
GeneIDi179945
KEGGicel:CELE_F23B12.5
UCSCiF23B12.5 c. elegans

Entry informationi

Entry nameiODP2_CAEEL
AccessioniPrimary (citable) accession number: Q19749
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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