Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Asparagine--tRNA ligase, cytoplasmic

Gene

nrs-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in protein synthesis (PubMed:23076791). Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.Curated1 Publication

Catalytic activityi

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).

GO - Molecular functioni

GO - Biological processi

  • asparaginyl-tRNA aminoacylation Source: InterPro
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • translation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine--tRNA ligase, cytoplasmic (EC:6.1.1.22)
Alternative name(s):
Asparaginyl-tRNA synthetase
Short name:
AsnRS
Gene namesi
Name:nrs-1
ORF Names:F22D6.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF22D6.3a; CE05684; WBGene00003815; nars-1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in an increase in the expression of gpdh-1 independent of hypertonic stress.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 545545Asparagine--tRNA ligase, cytoplasmicPRO_0000176495Add
BLAST

Proteomic databases

EPDiQ19722.
PaxDbiQ19722.
PRIDEiQ19722.

Interactioni

Protein-protein interaction databases

STRINGi6239.F22D6.3a.1.

Structurei

3D structure databases

ProteinModelPortaliQ19722.
SMRiQ19722. Positions 111-545.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0555. Eukaryota.
COG0017. LUCA.
GeneTreeiENSGT00550000074893.
HOGENOMiHOG000226035.
InParanoidiQ19722.
KOiK01893.
OMAiMEHRHLW.
OrthoDBiEOG7KQ21G.
PhylomeDBiQ19722.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF16. PTHR22594:SF16. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q19722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLYIDTDA GSDQHPGTEA QPLATLVQAM LISKNSGEFL MKKKSEEGES
60 70 80 90 100
WEPAAKAAIK KAVKKYEAEV KKLEKAGCRE KEAEEAQHAA LEEAKKITFS
110 120 130 140 150
LDKSLPEAKV IKIGESVQHR DQRVSIKAWV HRLRRQGKSL MFLVLRDGYG
160 170 180 190 200
FLQCVLNDKL CQSYDAVTLS TETSVQVYGI IKALPDGKSA PDGHELTVDY
210 220 230 240 250
WEVIGKAPAG GIDNVLNESA GVDVMLDNRH LVIRGENASR ILRIRAAATR
260 270 280 290 300
AMRDHFFAAG YTEVAPPTLV QTQVEGGSTL FGLDYYGEPA YLTQSSQLYL
310 320 330 340 350
ETCNAALGDV YCISQSYRAE KSRTRRHLSE YQHVEAECAF ITFDQLMDRI
360 370 380 390 400
EALVCDTVDR LLADPVTKSL IEFVNPGYKA PARPFKRMPY KEAIEWLQKN
410 420 430 440 450
DVRNEMGEKF VYGEDIAEAA ERRMTDTIGV PILLNRFPHG IKAFYMPRCA
460 470 480 490 500
DDNELTESVD LLMPGVGEIV GGSMRIWKED QLLAAFEKGG LDSKNYYWYM
510 520 530 540
DQRKYGSVPH GGYGLGLERF ICWLTDTNHI RDVCLYPRFV GRCAP
Length:545
Mass (Da):61,186
Last modified:November 1, 1996 - v1
Checksum:i53FADA68ECB81BAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71262 Genomic DNA. Translation: CAA95808.1.
PIRiT21253.
RefSeqiNP_001021405.1. NM_001026234.4.
UniGeneiCel.23239.

Genome annotation databases

EnsemblMetazoaiF22D6.3a; F22D6.3a; WBGene00003815.
GeneIDi172442.
KEGGicel:CELE_F22D6.3.
UCSCiF22D6.3a.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71262 Genomic DNA. Translation: CAA95808.1.
PIRiT21253.
RefSeqiNP_001021405.1. NM_001026234.4.
UniGeneiCel.23239.

3D structure databases

ProteinModelPortaliQ19722.
SMRiQ19722. Positions 111-545.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F22D6.3a.1.

Proteomic databases

EPDiQ19722.
PaxDbiQ19722.
PRIDEiQ19722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF22D6.3a; F22D6.3a; WBGene00003815.
GeneIDi172442.
KEGGicel:CELE_F22D6.3.
UCSCiF22D6.3a.1. c. elegans.

Organism-specific databases

CTDi172442.
WormBaseiF22D6.3a; CE05684; WBGene00003815; nars-1.

Phylogenomic databases

eggNOGiKOG0555. Eukaryota.
COG0017. LUCA.
GeneTreeiENSGT00550000074893.
HOGENOMiHOG000226035.
InParanoidiQ19722.
KOiK01893.
OMAiMEHRHLW.
OrthoDBiEOG7KQ21G.
PhylomeDBiQ19722.

Miscellaneous databases

NextBioi875533.
PROiQ19722.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF16. PTHR22594:SF16. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "GCN-2 dependent inhibition of protein synthesis activates osmosensitive gene transcription via WNK and Ste20 kinase signaling."
    Lee E.C., Strange K.
    Am. J. Physiol. 303:C1269-1277(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiSYNC_CAEEL
AccessioniPrimary (citable) accession number: Q19722
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 13, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.