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Q196Z5

- RIR1_IIV3

UniProt

Q196Z5 - RIR1_IIV3

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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

IIV3-065R

Organism
Invertebrate iridescent virus 3 (IIV-3) (Mosquito iridescent virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei67 – 671SubstrateBy similarity
Sitei83 – 831Important for hydrogen atom transferBy similarity
Sitei90 – 901Allosteric effector bindingBy similarity
Binding sitei111 – 1111Substrate; via amide nitrogenBy similarity
Sitei119 – 1191Allosteric effector bindingBy similarity
Active sitei317 – 3171Proton acceptorBy similarity
Active sitei319 – 3191Cysteine radical intermediateBy similarity
Active sitei321 – 3211Proton acceptorBy similarity
Sitei334 – 3341Important for hydrogen atom transferBy similarity
Sitei597 – 5971Important for electron transferBy similarity
Sitei598 – 5981Important for electron transferBy similarity
Sitei625 – 6251Interacts with thioredoxin/glutaredoxinBy similarity
Sitei628 – 6281Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
ORF Names:IIV3-065R
OrganismiInvertebrate iridescent virus 3 (IIV-3) (Mosquito iridescent virus)
Taxonomic identifieri345201 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageIridoviridaeChloriridovirus
Virus hostiAedes vexans (Inland floodwater mosquito) (Culex vexans) [TaxID: 7163]
Culex territans [TaxID: 42431]
Culiseta annulata [TaxID: 332058]
Ochlerotatus sollicitans (eastern saltmarsh mosquito) [TaxID: 310513]
Ochlerotatus taeniorhynchus (Black salt marsh mosquito) (Aedes taeniorhynchus) [TaxID: 329105]
Psorophora ferox [TaxID: 7183]
ProteomesiUP000001358: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 630630Ribonucleoside-diphosphate reductase large subunitPRO_0000376950Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi83 ↔ 334Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 832Substrate bindingBy similarity
Regioni317 – 3215Substrate bindingBy similarity
Regioni459 – 4635Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 2 hits.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.

Sequencei

Sequence statusi: Complete.

Q196Z5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MITIDESRNN LFDALGLQRL KDSYMKEDES SPQERFAFIA RQFCADDEPL
60 70 80 90 100
AQRLYDYMSQ HWLSPSSPQL SFGRTKQGLP IACFLPYLHD TARGLIDTWA
110 120 130 140 150
EVSELSMIGG GIGLGVGIRQ PDEKSVGIIP HLRTYDASCT AYKQGQTRRG
160 170 180 190 200
SYAAYLDISH PEILSFLNTR RVGGDHNYKL LNLHNGVNVP DSFMKKIWIL
210 220 230 240 250
STLAPFVKMD PCPTTETLFK KAVTTLKDSR YFGADDRWLG EVSFAALAEQ
260 270 280 290 300
LDVVNRWDLI DPHTGKVKET IKATELWERI ILTRAETGEP YIHWIDTSNR
310 320 330 340 350
ALPQFQKNLG LSIRQSNLCS EVVLPTDETR TAVCCLASLN LDYFDKWCNN
360 370 380 390 400
EQFYLDVATY LDNVLQYFID HAPPTLKRAV HSARSERAIG IGALGFHSYL
410 420 430 440 450
QSKMVDIESL PAYLINKKIF KTISSHLERV NLELGELRGE APDCVGTGRR
460 470 480 490 500
FSHMTAIAPN ATSSIIMGNT SPSCEPFRAN IYKQDTISGS FVTYNKHLKR
510 520 530 540 550
LLEERIPDNQ ARERVWSSIK MHDGSVQHLN QLTVQEKKVF KTWPEINQLS
560 570 580 590 600
LVMLAADRQK WIDQSQSTSL FFNPDERISY VHKIHLKAWL HGLKTLYYFR
610 620 630
SRKILTVDKV HHTTTTADPK TENDCTFCEG
Length:630
Mass (Da):71,757
Last modified:July 11, 2006 - v1
Checksum:iB4F309D57AD92677
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ643392 Genomic DNA. Translation: ABF82095.1.
RefSeqiYP_654637.1. NC_008187.1.

Genome annotation databases

GeneIDi4156315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ643392 Genomic DNA. Translation: ABF82095.1 .
RefSeqi YP_654637.1. NC_008187.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4156315.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 2 hits.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome of invertebrate iridescent virus type 3 (mosquito iridescent virus)."
    Delhon G., Tulman E.R., Afonso C.L., Lu Z., Becnel J.J., Moser B.A., Kutish G.F., Rock D.L.
    J. Virol. 80:8439-8449(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiRIR1_IIV3
AccessioniPrimary (citable) accession number: Q196Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: July 11, 2006
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3