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Q196Z5

- RIR1_IIV3

UniProt

Q196Z5 - RIR1_IIV3

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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene
IIV3-065R
Organism
Invertebrate iridescent virus 3 (IIV-3) (Mosquito iridescent virus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei67 – 671Substrate By similarity
Sitei83 – 831Important for hydrogen atom transfer By similarity
Sitei90 – 901Allosteric effector binding By similarity
Binding sitei111 – 1111Substrate; via amide nitrogen By similarity
Sitei119 – 1191Allosteric effector binding By similarity
Active sitei317 – 3171Proton acceptor By similarity
Active sitei319 – 3191Cysteine radical intermediate By similarity
Active sitei321 – 3211Proton acceptor By similarity
Sitei334 – 3341Important for hydrogen atom transfer By similarity
Sitei597 – 5971Important for electron transfer By similarity
Sitei598 – 5981Important for electron transfer By similarity
Sitei625 – 6251Interacts with thioredoxin/glutaredoxin By similarity
Sitei628 – 6281Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
ORF Names:IIV3-065R
OrganismiInvertebrate iridescent virus 3 (IIV-3) (Mosquito iridescent virus)
Taxonomic identifieri345201 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageIridoviridaeChloriridovirus
Virus hostiAedes vexans (Inland floodwater mosquito) (Culex vexans) [TaxID: 7163]
Culex territans [TaxID: 42431]
Culiseta annulata [TaxID: 332058]
Ochlerotatus sollicitans (eastern saltmarsh mosquito) [TaxID: 310513]
Ochlerotatus taeniorhynchus (Black salt marsh mosquito) (Aedes taeniorhynchus) [TaxID: 329105]
Psorophora ferox [TaxID: 7183]
ProteomesiUP000001358: Genome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 630630Ribonucleoside-diphosphate reductase large subunitPRO_0000376950Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi83 ↔ 334Redox-active By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 832Substrate binding By similarity
Regioni317 – 3215Substrate binding By similarity
Regioni459 – 4635Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 2 hits.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.

Sequencei

Sequence statusi: Complete.

Q196Z5-1 [UniParc]FASTAAdd to Basket

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MITIDESRNN LFDALGLQRL KDSYMKEDES SPQERFAFIA RQFCADDEPL    50
AQRLYDYMSQ HWLSPSSPQL SFGRTKQGLP IACFLPYLHD TARGLIDTWA 100
EVSELSMIGG GIGLGVGIRQ PDEKSVGIIP HLRTYDASCT AYKQGQTRRG 150
SYAAYLDISH PEILSFLNTR RVGGDHNYKL LNLHNGVNVP DSFMKKIWIL 200
STLAPFVKMD PCPTTETLFK KAVTTLKDSR YFGADDRWLG EVSFAALAEQ 250
LDVVNRWDLI DPHTGKVKET IKATELWERI ILTRAETGEP YIHWIDTSNR 300
ALPQFQKNLG LSIRQSNLCS EVVLPTDETR TAVCCLASLN LDYFDKWCNN 350
EQFYLDVATY LDNVLQYFID HAPPTLKRAV HSARSERAIG IGALGFHSYL 400
QSKMVDIESL PAYLINKKIF KTISSHLERV NLELGELRGE APDCVGTGRR 450
FSHMTAIAPN ATSSIIMGNT SPSCEPFRAN IYKQDTISGS FVTYNKHLKR 500
LLEERIPDNQ ARERVWSSIK MHDGSVQHLN QLTVQEKKVF KTWPEINQLS 550
LVMLAADRQK WIDQSQSTSL FFNPDERISY VHKIHLKAWL HGLKTLYYFR 600
SRKILTVDKV HHTTTTADPK TENDCTFCEG 630
Length:630
Mass (Da):71,757
Last modified:July 11, 2006 - v1
Checksum:iB4F309D57AD92677
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ643392 Genomic DNA. Translation: ABF82095.1.
RefSeqiYP_654637.1. NC_008187.1.

Genome annotation databases

GeneIDi4156315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ643392 Genomic DNA. Translation: ABF82095.1 .
RefSeqi YP_654637.1. NC_008187.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4156315.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 2 hits.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome of invertebrate iridescent virus type 3 (mosquito iridescent virus)."
    Delhon G., Tulman E.R., Afonso C.L., Lu Z., Becnel J.J., Moser B.A., Kutish G.F., Rock D.L.
    J. Virol. 80:8439-8449(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiRIR1_IIV3
AccessioniPrimary (citable) accession number: Q196Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: July 11, 2006
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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