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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

IIV3-065R

Organism
Invertebrate iridescent virus 3 (IIV-3) (Mosquito iridescent virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei67SubstrateBy similarity1
Sitei83Important for hydrogen atom transferBy similarity1
Sitei90Allosteric effector bindingBy similarity1
Binding sitei111Substrate; via amide nitrogenBy similarity1
Sitei119Allosteric effector bindingBy similarity1
Active sitei317Proton acceptorBy similarity1
Active sitei319Cysteine radical intermediateBy similarity1
Active sitei321Proton acceptorBy similarity1
Sitei334Important for hydrogen atom transferBy similarity1
Sitei597Important for electron transferBy similarity1
Sitei598Important for electron transferBy similarity1
Sitei625Interacts with thioredoxin/glutaredoxinBy similarity1
Sitei628Interacts with thioredoxin/glutaredoxinBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
ORF Names:IIV3-065R
OrganismiInvertebrate iridescent virus 3 (IIV-3) (Mosquito iridescent virus)
Taxonomic identifieri345201 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageIridoviridaeChloriridovirus
Virus hostiAedes vexans (Inland floodwater mosquito) (Culex vexans) [TaxID: 7163]
Culex territans [TaxID: 42431]
Culiseta annulata [TaxID: 332058]
Ochlerotatus sollicitans (eastern saltmarsh mosquito) [TaxID: 310513]
Ochlerotatus taeniorhynchus (Black salt marsh mosquito) (Aedes taeniorhynchus) [TaxID: 329105]
Psorophora ferox [TaxID: 7183]
Proteomesi
  • UP000001358 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003769501 – 630Ribonucleoside-diphosphate reductase large subunitAdd BLAST630

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi83 ↔ 334Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 83Substrate bindingBy similarity2
Regioni317 – 321Substrate bindingBy similarity5
Regioni459 – 463Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

KOiK00525.

Family and domain databases

InterProiIPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 2 hits.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.

Sequencei

Sequence statusi: Complete.

Q196Z5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITIDESRNN LFDALGLQRL KDSYMKEDES SPQERFAFIA RQFCADDEPL
60 70 80 90 100
AQRLYDYMSQ HWLSPSSPQL SFGRTKQGLP IACFLPYLHD TARGLIDTWA
110 120 130 140 150
EVSELSMIGG GIGLGVGIRQ PDEKSVGIIP HLRTYDASCT AYKQGQTRRG
160 170 180 190 200
SYAAYLDISH PEILSFLNTR RVGGDHNYKL LNLHNGVNVP DSFMKKIWIL
210 220 230 240 250
STLAPFVKMD PCPTTETLFK KAVTTLKDSR YFGADDRWLG EVSFAALAEQ
260 270 280 290 300
LDVVNRWDLI DPHTGKVKET IKATELWERI ILTRAETGEP YIHWIDTSNR
310 320 330 340 350
ALPQFQKNLG LSIRQSNLCS EVVLPTDETR TAVCCLASLN LDYFDKWCNN
360 370 380 390 400
EQFYLDVATY LDNVLQYFID HAPPTLKRAV HSARSERAIG IGALGFHSYL
410 420 430 440 450
QSKMVDIESL PAYLINKKIF KTISSHLERV NLELGELRGE APDCVGTGRR
460 470 480 490 500
FSHMTAIAPN ATSSIIMGNT SPSCEPFRAN IYKQDTISGS FVTYNKHLKR
510 520 530 540 550
LLEERIPDNQ ARERVWSSIK MHDGSVQHLN QLTVQEKKVF KTWPEINQLS
560 570 580 590 600
LVMLAADRQK WIDQSQSTSL FFNPDERISY VHKIHLKAWL HGLKTLYYFR
610 620 630
SRKILTVDKV HHTTTTADPK TENDCTFCEG
Length:630
Mass (Da):71,757
Last modified:July 11, 2006 - v1
Checksum:iB4F309D57AD92677
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ643392 Genomic DNA. Translation: ABF82095.1.
RefSeqiYP_654637.1. NC_008187.1.

Genome annotation databases

GeneIDi4156315.
KEGGivg:4156315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ643392 Genomic DNA. Translation: ABF82095.1.
RefSeqiYP_654637.1. NC_008187.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4156315.
KEGGivg:4156315.

Phylogenomic databases

KOiK00525.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

InterProiIPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 2 hits.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_IIV3
AccessioniPrimary (citable) accession number: Q196Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: July 11, 2006
Last modified: April 1, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.