Q196Z5 (RIR1_IIV3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase large subunit
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase large subunit

Gene names

ORF Names:

IIV3-065R

Organism

Invertebrate iridescent virus 3 (IIV-3) (Mosquito iridescent virus) [Reference proteome]

Taxonomic identifier

345201 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Iridoviridae › Chloriridovirus

Virus host

Aedes vexans (Inland floodwater mosquito) (Culex vexans) [TaxID: 7163]
Culex territans [TaxID: 42431]
Culiseta annulata [TaxID: 332058]
Ochlerotatus sollicitans (eastern saltmarsh mosquito) [TaxID: 310513]
Ochlerotatus taeniorhynchus (Black salt marsh mosquito) (Aedes taeniorhynchus) [TaxID: 329105]
Psorophora ferox [TaxID: 7183]

Protein attributes

Sequence length	630 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Enzyme regulation	Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family.

Ontologies

Keywords
Biological process	DNA replication
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 630	630	Ribonucleoside-diphosphate reductase large subunit		PRO_0000376950
Regions
	Region	82 – 83	2	Substrate binding By similarity
	Region	317 – 321	5	Substrate binding By similarity
	Region	459 – 463	5	Substrate binding By similarity
Sites
	Active site	317	1	Proton acceptor By similarity
	Active site	319	1	Cysteine radical intermediate By similarity
	Active site	321	1	Proton acceptor By similarity
	Binding site	67	1	Substrate By similarity
	Binding site	111	1	Substrate; via amide nitrogen By similarity
	Site	83	1	Important for hydrogen atom transfer By similarity
	Site	90	1	Allosteric effector binding By similarity
	Site	119	1	Allosteric effector binding By similarity
	Site	334	1	Important for hydrogen atom transfer By similarity
	Site	597	1	Important for electron transfer By similarity
	Site	598	1	Important for electron transfer By similarity
	Site	625	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	628	1	Interacts with thioredoxin/glutaredoxin By similarity
Amino acid modifications
	Disulfide bond	83 ↔ 334		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q196Z5 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: B4F309D57AD92677	FASTA	630	71,757
	10 20 30 40 50 60 MITIDESRNN LFDALGLQRL KDSYMKEDES SPQERFAFIA RQFCADDEPL AQRLYDYMSQ 70 80 90 100 110 120 HWLSPSSPQL SFGRTKQGLP IACFLPYLHD TARGLIDTWA EVSELSMIGG GIGLGVGIRQ 130 140 150 160 170 180 PDEKSVGIIP HLRTYDASCT AYKQGQTRRG SYAAYLDISH PEILSFLNTR RVGGDHNYKL 190 200 210 220 230 240 LNLHNGVNVP DSFMKKIWIL STLAPFVKMD PCPTTETLFK KAVTTLKDSR YFGADDRWLG 250 260 270 280 290 300 EVSFAALAEQ LDVVNRWDLI DPHTGKVKET IKATELWERI ILTRAETGEP YIHWIDTSNR 310 320 330 340 350 360 ALPQFQKNLG LSIRQSNLCS EVVLPTDETR TAVCCLASLN LDYFDKWCNN EQFYLDVATY 370 380 390 400 410 420 LDNVLQYFID HAPPTLKRAV HSARSERAIG IGALGFHSYL QSKMVDIESL PAYLINKKIF 430 440 450 460 470 480 KTISSHLERV NLELGELRGE APDCVGTGRR FSHMTAIAPN ATSSIIMGNT SPSCEPFRAN 490 500 510 520 530 540 IYKQDTISGS FVTYNKHLKR LLEERIPDNQ ARERVWSSIK MHDGSVQHLN QLTVQEKKVF 550 560 570 580 590 600 KTWPEINQLS LVMLAADRQK WIDQSQSTSL FFNPDERISY VHKIHLKAWL HGLKTLYYFR 610 620 630 SRKILTVDKV HHTTTTADPK TENDCTFCEG

References

[1]	"Genome of invertebrate iridescent virus type 3 (mosquito iridescent virus)." Delhon G., Tulman E.R., Afonso C.L., Lu Z., Becnel J.J., Moser B.A., Kutish G.F., Rock D.L. J. Virol. 80:8439-8449(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ643392 Genomic DNA. Translation: ABF82095.1.
RefSeq	YP_654637.1. NC_008187.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4156315.
Phylogenomic databases
ProtClustDB	CLSP2511488.
Enzyme and pathway databases
UniPathway	UPA00326.
Family and domain databases
InterPro	IPR000788. RNR_lg_C. IPR013509. RNR_lsu_N. IPR008926. RNR_R1-su_N. [Graphical view]
Pfam	PF02867. Ribonuc_red_lgC. 2 hits. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
SUPFAM	SSF48168. Ribonucleo_red_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIR1_IIV3

Accession

Primary (citable) accession number: Q196Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	July 11, 2006
Last modified:	January 9, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program