Q196X6 (VF232_IIV3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative ubiquitin thioesterase 232R
EC=3.4.19.12

Gene names

ORF Names:

IIV3-084L

Organism

Invertebrate iridescent virus 3 (IIV-3) (Mosquito iridescent virus) [Reference proteome]

Taxonomic identifier

345201 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Iridoviridae › Chloriridovirus

Virus host

Aedes vexans (Inland floodwater mosquito) (Culex vexans) [TaxID: 7163]
Culex territans [TaxID: 42431]
Culiseta annulata [TaxID: 332058]
Ochlerotatus sollicitans (eastern saltmarsh mosquito) [TaxID: 310513]
Ochlerotatus taeniorhynchus (Black salt marsh mosquito) (Aedes taeniorhynchus) [TaxID: 329105]
Psorophora ferox [TaxID: 7183]

Protein attributes

Sequence length	844 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, termination Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 844	844	Putative ubiquitin thioesterase 232R		PRO_0000377773
Regions
	Domain	590 – 725	136	OTU
	Compositional bias	137 – 218	82	Arg/Ser-rich
	Compositional bias	347 – 351	5	Poly-Pro
	Compositional bias	428 – 452	25	Pro-rich
	Compositional bias	747 – 806	60	Pro-rich
Sites
	Active site	598	1	Potential
	Active site	601	1	Nucleophile By similarity
	Active site	718	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q196X6 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 6E24E9C3EE3C5872	FASTA	844	93,521
	10 20 30 40 50 60 MCDVDLKTCQ KSSKFKKKDI VELGKQCGVN PYLSNGKEKS RTVICTEIVA SYNPPPGSSQ 70 80 90 100 110 120 GDDSEHESIS QVNNIPNRNE KFLPKSKYPT YTQVLNMEKR QLKALAKKLG LEYKKQTEQQ 130 140 150 160 170 180 LQGAINLKIK TLEGLNSSTR SRSPSVRSRC RSPSPRAPSV RSRLPSTRSR CRSPSPRAPS 190 200 210 220 230 240 TRSRSPSVRS RCRSPSPRAP SVRSRSPSRQ SVRQSSESAD EAEQVALETM KTAHLRMLAT 250 260 270 280 290 300 TLGASTVTGM KKKDLIDYIK SRRKSPSPSP VPPSTRCDPT TTAPPMEDLF KKKVDELKTM 310 320 330 340 350 360 AKNAGFVRWN GKTLSKMNKS DLVDFLLNGM NRPSPSLPQS RSRTRSPPPP PRSRSPSVGS 370 380 390 400 410 420 PSVRDGGAGR RRLPELTRAQ LTAMKVVDLK AMATELGLTR YRGMNRTQMR KGDVINFIIE 430 440 450 460 470 480 TQKKQKTPSP SPTPPSPVPS VVGSRRPKSP LPYKSRDFVA LADDDSEPGV EVQKKMGKSG 490 500 510 520 530 540 EREPKSVPNV RIIPSEIPAP TEGSLRSRLS TQQQTQQSVV YEDPNESIKP EESVRAPKLS 550 560 570 580 590 600 VVDPQLSRKT LKPLPSLVVT DQPSKQPELP KYKGRTPYTD LEQLAQSKGY TVKQVSGDGN 610 620 630 640 650 660 CLFRSVCKSI RALRGEKFTH RQLRQMVVDY LRENPEFLQV YLEYVARQRD NSLPSTEQYL 670 680 690 700 710 720 SEMSKCGTWG DLICLKTLSE ILKVQFNLLI LNTKQFQMVS SQDDYPDVIP LGYIDNYHYT 730 740 750 760 770 780 SLVPIGLDSK GGAASSTTTG LKQLDGPRPP ITLIPESQVP AVAATISTQQ PPSIVAPPIS 790 800 810 820 830 840 VGGSQLVPSI VPQPQMPKPD FKPVKPLSNL NELLDLMDRV KPQVYNDISQ LEKARQSIKV SLGL

References

[1]	"Genome of invertebrate iridescent virus type 3 (mosquito iridescent virus)." Delhon G., Tulman E.R., Afonso C.L., Lu Z., Becnel J.J., Moser B.A., Kutish G.F., Rock D.L. J. Virol. 80:8439-8449(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ643392 Genomic DNA. Translation: ABF82114.1.
RefSeq	YP_654656.1. NC_008187.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4156295.
Phylogenomic databases
ProtClustDB	CLSP2511671.
Family and domain databases
InterPro	IPR003323. OTU. IPR011112. Rho_N. [Graphical view]
Pfam	PF02338. OTU. 1 hit. PF07498. Rho_N. 1 hit. [Graphical view]
SMART	SM00959. Rho_N. 3 hits. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VF232_IIV3

Accession

Primary (citable) accession number: Q196X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	July 11, 2006
Last modified:	October 3, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program