Reviewed, UniProtKB/Swiss-Prot Q195N6 (PDRP1_MAIZE)

Names and origin

Protein names

Recommended name:
    Pyruvate, phosphate dikinase regulatory protein, chloroplastic
    EC=2.7.11.n1
    EC=2.7.4.n1
Alternative name(s):
    Pyruvate, Pi dikinase regulatory protein
      Short name=PPDK regulatory protein
      Short name=PPDK RP
    Bifunctional dikinase regulatory protein
      Short name=BFRP

Gene names

Name:

PDRP1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Bifunctional serine/threonine kinase and phosphorylase involved in the dark/light-mediated regulation of PPDK by catalyzing its phosphorylation/dephosphorylation. Dark/light-induced changes in stromal concentrations of the competing ADP and Pi substrates govern the direction of the reaction. In the dark, phosphorylates the catalytic intermediate of PPDK (PPDK-HisP), inactivating it. Light exposure induces the phosphorolysis reaction that reactivates PPDK. Ref.2 Ref.4 Ref.6
Catalytic activity	ADP + [Pyruvate, phosphate dikinase] = AMP + [pyruvate, phosphate dikinase] phosphate. Ref.3 [Pyruvate, phosphate dikinase] phosphate + phosphate = [pyruvate, phosphate dikinase] + diphosphate. Ref.3
Enzyme regulation	Regulated by light/dark exposure.
Pathway	Photosynthesis; C4 acid pathway.
Subcellular location	Plastid › chloroplast stroma. Ref.5
Tissue specificity	Leaf mesophyll-cells.
Sequence similarities	Belongs to the pyruvate, phosphate dikinase regulatory protein family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphotransferase activity, phosphate group as acceptor Inferred from electronic annotation. Source: EC protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 41	41	Chloroplast Potential
	Chain	42 – 426	385	Pyruvate, phosphate dikinase regulatory protein, chloroplastic		PRO_0000315393
Regions
	Nucleotide binding	153 – 160	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q195N6-1 [UniParc]. Last modified July 11, 2006. Version 1. Checksum: 99F6F7B2B0D9F6D6	FASTA	426	45,905
	10 20 30 40 50 60 MIGCAKPLAA PLQAWARPPS PAGRRLPPSF CAPDTSPALT RAVESPGQSQ SDDAPPPRSG 70 80 90 100 110 120 EAASSLAPRA SSHLDRWSRS RALRSGHRPA LNRAALSSAS VSAPPVIKSP RPEDAAVAAE 130 140 150 160 170 180 DGEDDDVCEA ERDAAAGKAI YIVSDGTGWT AEHSVNAALG QFENCLADRG CAVNTHLFSL 190 200 210 220 230 240 IDDMDRLIEV IKQAAKEGAL VLYTLADPSM AEATKKACDF WGVPCTDVLR PTVEAIASHI 250 260 270 280 290 300 GVAPSGIPRS FPSRNGRLSE DYFQRIDAID FTIKQDDGVL PQNFYRADIV LAGVSRTGKT 310 320 330 340 350 360 PLSIYLAQKG YKVANVPIVM GVALPKSLFE INQDKVFGLT INPAILQGIR KTRAKTLGFD 370 380 390 400 410 420 GRQSNYAEMD HVRQELVHAN QIFVQNPWWP VIAVTGKAIE ETAAVILGIL HDRKQKCSMP RISKRY

References

[1]	"Cloning and expression of maize-leaf pyruvate, Pi dikinase regulatory protein gene." Burnell J.N., Chastain C.J. Biochem. Biophys. Res. Commun. 345:675-680(2006) [PubMed: 16696949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], EXPRESSION, CHARACTERIZATION. Tissue: Leaf.
[2]	"Regulation of C4 photosynthesis: identification of a catalytically important histidine residue and its role in the regulation of pyruvate,Pi dikinase." Burnell J.N., Hatch M.D. Arch. Biochem. Biophys. 231:175-182(1984) [PubMed: 6326674] [Abstract] Cited for: FUNCTION.
[3]	"Regulation of C4 photosynthesis: purification and properties of the protein catalyzing ADP-mediated inactivation and Pi-mediated activation of pyruvate,Pi dikinase." Burnell J.N., Hatch M.D. Arch. Biochem. Biophys. 237:490-503(1985) [PubMed: 2983615] [Abstract] Cited for: CATALYTIC ACTIVITY.
[4]	"Sequence of the phosphothreonyl regulatory site peptide from inactive maize leaf pyruvate, orthophosphate dikinase." Roeske C.A., Kutny R.M., Budde R.J.A., Chollet R. J. Biol. Chem. 263:6683-6687(1988) [PubMed: 2834385] [Abstract] Cited for: FUNCTION.
[5]	"Partial purification and characterization of maize-leaf pyruvate, orthophosphate dikinase regulatory protein: a low-abundance, mesophyll-chloroplast stromal protein." Smith C.M., Duff S.M., Chollet R. Arch. Biochem. Biophys. 308:200-206(1994) [PubMed: 8311453] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Further analysis of maize C(4) pyruvate,orthophosphate dikinase phosphorylation by its bifunctional regulatory protein using selective substitutions of the regulatory Thr-456 and catalytic His-458 residues." Chastain C.J., Botschner M., Harrington G.E., Thompson B.J., Mills S.E., Sarath G., Chollet R. Arch. Biochem. Biophys. 375:165-170(2000) [PubMed: 10683263] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ645424 mRNA. Translation: ABG00151.1.
RefSeq	NP_001105873.1.
UniGene	Zm.10212
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	732785.
Organism-specific databases
MaizeGDB	293064.
Family and domain databases
InterPro	IPR005177. DUF299_PTrfase_pred. IPR017409. Pyrv_Pi_dikinase_reg. [Graphical view]
Pfam	PF03618. DUF299. 1 hit. [Graphical view]
PIRSF	PIRSF038149. Pyruvate_Pi_dikinase_regulator. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PDRP1_MAIZE

Accession

Primary (citable) accession number: Q195N6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 11, 2006
Last modified:	October 13, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)