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Protein

Guanine nucleotide-binding protein alpha-12 subunit

Gene

gpa-12

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (Probable). May play a role in resistance to fungal infection in the epidermis by regulating the up-regulation of several antimicrobial peptides of the NLP and CNC families (PubMed:22470487, PubMed:19380113). Upstream of plc-3, tpa-1 and the p38-like pathway, required for the expression of antimicrobial peptide nlp-29 in the epidermis in response to fungal infection or physical injury (PubMed:19380113).Curated2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43MagnesiumBy similarity1
Metal bindingi182MagnesiumBy similarity1
Binding sitei327GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43GTPBy similarity8
Nucleotide bindingi176 – 182GTPBy similarity7
Nucleotide bindingi201 – 205GTPBy similarity5
Nucleotide bindingi270 – 273GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransducer
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-193648. NRAGE signals death through JNK.
R-CEL-194840. Rho GTPase cycle.
R-CEL-416482. G alpha (12/13) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein alpha-12 subunit
Gene namesi
Name:gpa-12
ORF Names:F18G5.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiF18G5.3; CE20692; WBGene00001674; gpa-12.

Subcellular locationi

GO - Cellular componenti

  • brush border membrane Source: GO_Central
  • cytoplasm Source: WormBase
  • heterotrimeric G-protein complex Source: GO_Central
  • nucleus Source: WormBase

Pathology & Biotechi

Disruption phenotypei

Up-regulation of nlp-29 is severely impaired in the hyp7 epidermal cell but not in vulva epidermal cells following fungal infection by D.coniospora or physical injury.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi205Q → L: Probably constitutively active. Constitutive expression of nlp-29, nlp-31, nlp-34, cnc-2, cnc-4 and cnc-1 in absence of infection. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002036491 – 355Guanine nucleotide-binding protein alpha-12 subunitAdd BLAST355

Proteomic databases

EPDiQ19572.
PaxDbiQ19572.
PeptideAtlasiQ19572.

Expressioni

Gene expression databases

BgeeiWBGene00001674.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi6239.F18G5.3.

Structurei

3D structure databases

ProteinModelPortaliQ19572.
SMRiQ19572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family.Curated

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00770000120503.
HOGENOMiHOG000038729.
InParanoidiQ19572.
KOiK04346.
OMAiKEYQHVI.
OrthoDBiEOG091G0NV2.
PhylomeDBiQ19572.

Family and domain databases

CDDicd00066. G-alpha. 1 hit.
Gene3Di1.10.400.10. 1 hit.
InterProiView protein in InterPro
IPR000469. Gprotein_alpha_12/13.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiView protein in Pfam
PF00503. G-alpha. 1 hit.
PRINTSiPR00318. GPROTEINA.
PR00440. GPROTEINA12.
SMARTiView protein in SMART
SM00275. G_alpha. 1 hit.
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q19572-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVCCFGKKDE RTKTIEKELH KERKIMRRQI NLLLLGSGES GKSTFVKQMH
60 70 80 90 100
IIHGAGEFTA DEVRAYRQQI YQNAISAMRV LLDARNKLGI AWEDPKRQVE
110 120 130 140 150
VEKVMRFSVG DLLKGIDFTT FVEVAPIISD FWNDAAIRKT YEQRNLFQIS
160 170 180 190 200
DSCQYFFEHI PRIAMPDFYP TNRDILFCRK ATRGISEHIF EINKIPFRFI
210 220 230 240 250
DVGGQRSQRQ KWFQCFTDIT SILFMVASNE YDQVILEDRR TNRVVESRSV
260 270 280 290 300
FETIVNNRAF SNVSIILFMN KNDLLQEKVP KSDIRQYFTD FTGDHTLVRD
310 320 330 340 350
VQFFLVDKFE ASRRDRARPF FYHFTTAVDT ENIRRVFRDV RESILEQNLK

TLMMQ
Length:355
Mass (Da):41,974
Last modified:November 1, 1999 - v2
Checksum:i40DD3793B8132E69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY008134 mRNA. Translation: AAG32087.1.
FO080202 Genomic DNA. Translation: CCD61930.1.
PIRiD89605.
RefSeqiNP_509557.1. NM_077156.3.
UniGeneiCel.805.

Genome annotation databases

EnsemblMetazoaiF18G5.3; F18G5.3; WBGene00001674.
GeneIDi181157.
KEGGicel:CELE_F18G5.3.
UCSCiF18G5.3. c. elegans.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGPA12_CAEEL
AccessioniPrimary (citable) accession number: Q19572
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: November 1, 1999
Last modified: June 7, 2017
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families