Reviewed, UniProtKB/Swiss-Prot Q19564 (OXDD1_CAEEL)

Names and origin

Protein names

Recommended name:
    D-aspartate oxidase 1
      Short name=DASOX 1
    EC=1.4.3.1
Alternative name(s):
    DDO 1

Gene names

ORF Names:

F18E3.7

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Selectively catalyzes the oxidative deamination of D-aspartate and its N-methylated derivative, N-methyl D-aspartate.
Catalytic activity	D-aspartate + H(2)O + O(2) = oxaloacetate + NH(3) + H(2)O(2).
Cofactor	FAD By similarity.
Sequence similarities	Belongs to the DAMOX/DASOX family.
Caution	The conserved active site Tyr residue in position 224 is replaced by a Phe.
Biophysicochemical properties	Kinetic parameters: KM=0.38 mM for D-Asp KM=0.25 mM for D-Glu KM=1.84 mM for NMDA Vmax=4.40 µmol/min/mg enzyme with D-Asp as substrate Vmax=3.03 µmol/min/mg enzyme with D-Glu as substrate Vmax=4.31 µmol/min/mg enzyme with NMDA as substrate

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid oxidase activity Inferred from electronic annotation. Source: InterPro D-aspartate oxidase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q19564-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Notes: No experimental confirmation available.

Isoform b (identifier: Q19564-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-222: Missing.
     223-228: TFTIPK → MCQIFR
     268-273: EPKIIK → VRISFF
     274-334: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	D-aspartate oxidase 1		PRO_0000162772
Regions
	Nucleotide binding	8 – 22	15	FAD Potential
Sites
	Active site	305	1	By similarity
Natural variations
	Alternative sequence	1 – 222	222	Missing in isoform b.		VSP_014356
	Alternative sequence	223 – 228	6	TFTIPK → MCQIFR in isoform b.		VSP_014357
	Alternative sequence	268 – 273	6	EPKIIK → VRISFF in isoform b.		VSP_014358
	Alternative sequence	274 – 334	61	Missing in isoform b.		VSP_014359

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 90B1731A702BB6FD	FASTA	334	37,608
	10 20 30 40 50 60 MANIIPKIAI IGEGVIGCTS ALQISKAIPN AKITVLHDKP FKKSCSAGPA GLFRIDYEEN 70 80 90 100 110 120 TEYGRASFAW FSHLYRTTKG SETGVKLVSG HIQSDNLESL KQQQRAYGDI VYNFRFLDDR 130 140 150 160 170 180 ERLDIFPEPS KHCIHYTAYA SEGNKYVPYL KNLLLEQKIE FKQQEVTSLD AVADAGYDVI 190 200 210 220 230 240 VNCAGLYGGK LAGDDDTCYP IRGVILEVDA PWHKHFNYRD FTTFTIPKEH SVVVGSTKQD 250 260 270 280 290 300 NRWDLEITDE DRNDILKRYI ALHPGMREPK IIKEWSALRP GRKHVRIEAQ KRTSVGNSKD 310 320 330 YMVVHHYGHG SNGFTLGWGT AIEATKLVKT ALGL
	Isoform b [UniParc]. Checksum: 5DADC95508918047		51	6,137
	10 20 30 40 50 MCQIFREHSV VVGSTKQDNR WDLEITDEDR NDILKRYIAL HPGMRVRISF F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Caenorhabditis elegans has two genes encoding functional D-aspartate oxidases." Katane M., Seida Y., Sekine M., Furuchi T., Homma H. FEBS J. 274:137-149(2007) [PubMed: 17140416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB275892 mRNA. Translation: BAF34315.1. U53139 Genomic DNA. Translation: AAK18939.1. U53139 Genomic DNA. Translation: AAN84824.1.
PIR	T29219.
RefSeq	NP_504908.1.
UniGene	Cel.4023
3D structure databases
HSSP	HSSP built from PDB template 1AN9 based on UniProtKB P00371.
ModBase	Search...
Genome annotation databases
Ensembl	F18E3.7. Caenorhabditis elegans. [Contig view]
GeneID	179130.
KEGG	cel:F18E3.7.
NMPDR	fig|6239.3.peg.18465.
Organism-specific databases
WormBase	WBGene00017565. F18E3.7.
WormPep	F18E3.7a. CE07083. [WorfDB] F18E3.7b. CE32864. [WorfDB]
Gene expression databases
ArrayExpress	Q19564.
Family and domain databases
InterPro	IPR006181. D-amino_acid_oxidase_CS. IPR006076. FAD-dep_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PROSITE	PS00677. DAO. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	904050.

Entry information

Entry name

OXDD1_CAEEL

Accession

Primary (citable) accession number: Q19564
Secondary accession number(s): Q08I99, Q8I7K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project