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Protein

Probable Werner syndrome ATP-dependent helicase homolog 1

Gene

wrn-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Essential for the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity. May be involved in the control of genomic stability (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi249 – 2568ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' DNA helicase activity Source: WormBase
  • DNA binding Source: UniProtKB-KW

GO - Biological processi

  • determination of adult lifespan Source: WormBase
  • DNA duplex unwinding Source: GOC
  • DNA metabolic process Source: WormBase
  • DNA recombination Source: InterPro
  • DNA repair Source: InterPro
  • DNA replication Source: InterPro
  • growth Source: WormBase
  • response to ionizing radiation Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.12. 1045.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable Werner syndrome ATP-dependent helicase homolog 1 (EC:3.6.4.12)
Gene namesi
Name:wrn-1
ORF Names:F18C5.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiF18C5.2; CE31791; WBGene00006944; wrn-1.

Subcellular locationi

GO - Cellular componenti

  • chromosome Source: WormBase
  • nucleoplasm Source: WormBase
  • nucleus Source: WormBase
  • site of double-strand break Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10561056Probable Werner syndrome ATP-dependent helicase homolog 1PRO_0000205048Add
BLAST

Proteomic databases

EPDiQ19546.
PaxDbiQ19546.
PRIDEiQ19546.

Interactioni

Protein-protein interaction databases

BioGridi39420. 5 interactions.
IntActiQ19546. 4 interactions.
MINTiMINT-226609.
STRINGi6239.F18C5.2.

Structurei

3D structure databases

ProteinModelPortaliQ19546.
SMRiQ19546. Positions 203-766.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati17 – 261011 Publication
Repeati28 – 371021 Publication
Domaini236 – 406171Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini427 – 583157Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini806 – 88681HRDCPROSITE-ProRule annotationCuratedAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 37212 X 10 AA repeats of N-[ED]-E-L-P-E-T-E-P-EAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi348 – 3514DEAH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 74Poly-Asp
Compositional biasi90 – 956Poly-Ser
Compositional biasi628 – 6314Poly-Ser

Sequence similaritiesi

Belongs to the helicase family. RecQ subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 HRDC domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0351. Eukaryota.
COG0514. LUCA.
GeneTreeiENSGT00550000074520.
HOGENOMiHOG000016918.
InParanoidiQ19546.
KOiK10900.
OMAiPANENEQ.
OrthoDBiEOG7J70F2.
PhylomeDBiQ19546.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.80. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR029491. Helicase_HTH.
IPR010997. HRDC-like.
IPR002121. HRDC_dom.
IPR027417. P-loop_NTPase.
IPR032284. RecQ_Zn-bd.
IPR018982. RQC_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00570. HRDC. 1 hit.
PF14493. HTH_40. 1 hit.
PF16124. RecQ_Zn_bind. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00341. HRDC. 1 hit.
SM00956. RQC. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47819. SSF47819. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00614. recQ_fam. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50967. HRDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q19546-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISDDDDLPS TRPGSVNEEL PETEPEDNDE LPETEPESDS DKPTVTSNKT
60 70 80 90 100
ENQVADEDYD SFDDFVPSQT HTASKIPVKN KRAKKCTVES DSSSSDDSDQ
110 120 130 140 150
GDDCEFIPAC DETQEVPKIK RGYTLRTRAS VKNKCDDSWD DGIDEEDVSK
160 170 180 190 200
RSEDTLNDSF VDPEFMDSVL DNQLTIKGKK QFLDDGEFFT DRNVPQIDEA
210 220 230 240 250
TKMKWASMTS PPQEALNALN EFFGHKGFRE KQWDVVRNVL GGKDQFVLMS
260 270 280 290 300
TGYGKSVCYQ LPSLLLNSMT VVVSPLISLM NDQVTTLVSK GIDAVKLDGH
310 320 330 340 350
STQIEWDQVA NNMHRIRFIY MSPEMVTSQK GLELLTSCRK HISLLAIDEA
360 370 380 390 400
HCVSQWGHDF RNSYRHLAEI RNRSDLCNIP MIALTATATV RVRDDVIANL
410 420 430 440 450
RLRKPLITTT SFDRKNLYIS VHSSKDMAED LGLFMKTDEV KGRHFGGPTI
460 470 480 490 500
IYCQTKQMVD DVNCVLRRIG VRSAHYHAGL TKNQREKAHT DFMRDKITTI
510 520 530 540 550
VATVAFGMGI DKPDVRNVIH YGCPNNIESY YQEIGRAGRD GSPSICRVFW
560 570 580 590 600
APKDLNTIKF KLRNSQQKEE VVENLTMMLR QLELVLTTVG CRRYQLLKHF
610 620 630 640 650
DPSYAKPPTM QADCCDRCTE MLNGNQDSSS SIVDVTTESK WLFQVINEMY
660 670 680 690 700
NGKTGIGKPI EFLRGSSKED WRIKTTSQQK LFGIGKHIPD KWWKALAASL
710 720 730 740 750
RIAGYLGEVR LMQMKFGSCI TLSELGERWL LTGKEMKIDA TPILLQGKKE
760 770 780 790 800
KAAPSTVPGA SRSQSTKSST EIPTKILGAN KIREYEPANE NEQLMNLKKQ
810 820 830 840 850
EVTGLPEKID QLRSRLDDIR VGIANMHEVA PFQIVSNTVL DCFANLRPTS
860 870 880 890 900
ASNLEMIDGM SAQQKSRYGK RFVDCVVQFS KETGIATNVN ANDMIPPELI
910 920 930 940 950
SKMQKVLSDA VRRVYTEHLI SRSTAKEVAT ARGISEGTVY SYLAMAVEKG
960 970 980 990 1000
LPLHLDKLNV SRKNIAMALN AVRVHLGSNV AVLTPWVEAM GVVPDFNQLK
1010 1020 1030 1040 1050
LIRAILIYEY GLDTSENQEK PDIQSMPSTS NPSTIKTVPS TPSSSLRAPP

LKKFKL
Length:1,056
Mass (Da):118,523
Last modified:October 1, 2001 - v2
Checksum:i99C23E70D06DAA0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080970 Genomic DNA. Translation: CCD68193.1.
PIRiT16087.
RefSeqiNP_495324.2. NM_062923.5.
UniGeneiCel.7245.

Genome annotation databases

EnsemblMetazoaiF18C5.2; F18C5.2; WBGene00006944.
GeneIDi174081.
KEGGicel:CELE_F18C5.2.
UCSCiF18C5.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080970 Genomic DNA. Translation: CCD68193.1.
PIRiT16087.
RefSeqiNP_495324.2. NM_062923.5.
UniGeneiCel.7245.

3D structure databases

ProteinModelPortaliQ19546.
SMRiQ19546. Positions 203-766.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi39420. 5 interactions.
IntActiQ19546. 4 interactions.
MINTiMINT-226609.
STRINGi6239.F18C5.2.

Proteomic databases

EPDiQ19546.
PaxDbiQ19546.
PRIDEiQ19546.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF18C5.2; F18C5.2; WBGene00006944.
GeneIDi174081.
KEGGicel:CELE_F18C5.2.
UCSCiF18C5.2. c. elegans.

Organism-specific databases

CTDi174081.
WormBaseiF18C5.2; CE31791; WBGene00006944; wrn-1.

Phylogenomic databases

eggNOGiKOG0351. Eukaryota.
COG0514. LUCA.
GeneTreeiENSGT00550000074520.
HOGENOMiHOG000016918.
InParanoidiQ19546.
KOiK10900.
OMAiPANENEQ.
OrthoDBiEOG7J70F2.
PhylomeDBiQ19546.

Enzyme and pathway databases

BRENDAi3.6.4.12. 1045.

Miscellaneous databases

PROiQ19546.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.80. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR029491. Helicase_HTH.
IPR010997. HRDC-like.
IPR002121. HRDC_dom.
IPR027417. P-loop_NTPase.
IPR032284. RecQ_Zn-bd.
IPR018982. RQC_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00570. HRDC. 1 hit.
PF14493. HTH_40. 1 hit.
PF16124. RecQ_Zn_bind. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00341. HRDC. 1 hit.
SM00956. RQC. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47819. SSF47819. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00614. recQ_fam. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50967. HRDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. "Evolution of the RECQ family of helicases: a Drosophila homolog, Dmblm, is similar to the human Bloom syndrome gene."
    Kusano K., Berres M.E., Engels W.R.
    Genetics 151:1027-1039(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: REPEATS.

Entry informationi

Entry nameiWRN_CAEEL
AccessioniPrimary (citable) accession number: Q19546
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: October 1, 2001
Last modified: June 8, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.